Engineering the substrate specificity and reactivity of a heme protein: creation of an ascorbate binding site in cytochrome c peroxidase

Biochemistry

Volumn 47, Issue 52, 2008, Pages 13933-13941

  Murphy, Emma J ^a   Metcalfe, Clive L ^a   Basran, Jaswir ^a   Moody, Peter C E ^a   Raven, Emma Lloyd ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ASCORBATE PEROXIDASE; CATION-RADICALS; COMPOUND I; CRYSTALLOGRAPHIC DATA; CYTOCHROME C; HEME ENZYME; HEME PROPIONATES; HEME PROTEINS; MECHANISTIC STUDIES; STEADY-STATE CONDITION; STRUCTURAL CHANGE; SUBSTRATE BINDING; SUBSTRATE OXIDATION; SUBSTRATE SPECIFICITY;

BINDING ENERGY; ENZYMES; HEMOGLOBIN; HYDROGEN BONDS; OXIDATION; REACTION INTERMEDIATES; SUBSTRATES;

PORPHYRINS;

ASCORBATE PEROXIDASE; CYTOCHROME C PEROXIDASE; HEMOPROTEIN; ASCORBIC ACID;

ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ENZYME SPECIFICITY; HYDROGEN BOND; MUTATION; OXIDATION; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STEADY STATE; TURNOVER TIME; AMINO ACID SUBSTITUTION; GENETICS; METABOLISM; OXIDATION REDUCTION REACTION; X RAY CRYSTALLOGRAPHY;

AMINO ACID SUBSTITUTION; ASCORBIC ACID; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; CYTOCHROME-C PEROXIDASE; HEMEPROTEINS; OXIDATION-REDUCTION; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

EID: 58849088119     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi801480r     Document Type: Article

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