Immunophenotyping and DNA content analysis of acetone-fixed cells

Current Protocols in Cytometry

Volumn , Issue SUPPL. 46, 2008, Pages

  Mancaniello, Debora ^a   Carbonari, Maurizio ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Conservative fixation; DNA content; Multiparametric flow cytometry; Proliferating cells

Indexed keywords

ACETONE; DNA;

CELL CYCLE PHASE; CELL MEMBRANE PERMEABILITY; CELL PROLIFERATION; CELL STRUCTURE; CELL SUBPOPULATION; CONTROLLED STUDY; CYTOLOGY; CYTOMETRY; DNA CONTENT; DNA DETERMINATION; FLOW CYTOMETRY; IMMUNOPHENOTYPING; PLOIDY; PRIORITY JOURNAL; REVIEW; STAINING; STOICHIOMETRY; TEMPERATURE SENSITIVITY;

EID: 58749104559     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cy0926s46     Document Type: Review

References (10)

1. Darzynkiewicz, Z., Juan, G., and Bedner, E. 1999. Determining cell cycle stages by flowcytometry. Curr. Protoc. Cell Biol. 1:8.4.1-8.4.18.
2. Eudey, T.L. 1996. Statistical considerations in DNA flow cytometry. Stat. Sci. 11:320-324.
3. Hudson, L. and Hay, F.C. 1989. Practical Immunology, 3rd. ed. Blackwell Scientific Publications, Oxford, U.K.
4. Lanuti, P., Marchisio, M., Cantilena, S., Paludi, M., Bascelli, A., Gaspari, A.R., Grifone, G., Centurione, M.A., Papa, S., Di Pietro, R., Cataldi, A.,Miscia, S., and Bertagnolo, V. 2006. A flow cytometry procedure for simultaneous characterization of cell DNA content and expression of intracellular protein kinase C-ζ. Immunol. Meth. 315:37-48.
5. Rabinovitch, P.S., Torres, R.M., and Engel, D. 1986. Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: Applications to study of cell activation and the cell cycle of murine ly-1 B cells. J. Immunol. 136:2769-2775.
6. Rousselle, C., Robert-Nicoud, M., and Ronot, X. 1998. Flow cytometric analysis of DNA content of living and fixed cells: A comparative study using various fixatives. Histochem. J. 30:773-781.
7. Sato, Y., Mukai, K., Watanabe, S., Goto, M., and Shimosato, Y. 1986. The amex method. A semplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining. Am. J. Pathol. 125:431-435.
8. Schimenti, K.J. and Jacobberger, J.W. 1992. Fixation of mammalian cells for flow cytometric evaluation of DNA content and nuclear immunofluorescence. Cytometry 13:48-59.
9. Tanaka, M., Tanaka, H., and Ishikawa, E. 1984. Immunohistochemical demonstration of surface antigen of human lymphocytes with monoclonal antibody in acetone-fixed paraffin-embedded sections. J. Histochem. Cytochem. 32:452-454.
10. Telford, G.W., King, L.E., and Fraker, P.J. 1994. Rapid quantitation of apoptosis in pure and heterogeneous cell populations using flow cytometry. J. Immunol. Meth. 172:1-16.