Efficient protocol for purification of diosgenin and two fatty acids from Rhizoma dioscoreae by SFE coupled with high-speed counter-current chromatography and evaporative light scattering detection

Journal of Separation Science

Volumn 31, Issue 20, 2008, Pages 3638-3646

  Xu, Youwei ^a   Han, Xu ^a   Dong, Deshi ^a   Xu, Lina ^a   Qi, Yan ^a   Peng, Jinyong ^a   Zhan, Libin ^a  

^a DALIAN MEDICAL UNIVERSITY   (China)

Author keywords

Counter current chromatography; Diosgenin; Rhizoma dioscoreae; SFE

Indexed keywords

ACID HYDROLYSIS; COUNTERCURRENT CHROMATOGRAPHY; CRUDE EXTRACT; DIOSGENIN; DYNAMIC EXTRACTION; EVAPORATIVE LIGHT SCATTERING DETECTION; HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY; LINOLENIC ACIDS; MOBILE PHASE; N-HEXANE; ONE-STEP PURIFICATION; RHIZOMA DIOSCOREAE; SFE; SOLVENT SYSTEM; STATIONARY PHASE; TRADITIONAL CHINESE MEDICINE; TWO-PHASE SOLVENT SYSTEM; VOLUME RATIO;

CARBON DIOXIDE; ETHANOL; LIGHT SCATTERING; LINOLEIC ACID; PURIFICATION; SEPARATION; SOLVENTS; SUPERCRITICAL FLUID EXTRACTION;

CHROMATOGRAPHY;

DIOSGENIN; FATTY ACID; PLANT EXTRACT;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; CHINESE MEDICINE; COUNTER CURRENT CHROMATOGRAPHY; EVALUATION; INSTRUMENTATION; ISOLATION AND PURIFICATION; MEDICINAL PLANT; METHODOLOGY; SUPERCRITICAL FLUID CHROMATOGRAPHY; YAM;

CHROMATOGRAPHY, SUPERCRITICAL FLUID; COUNTERCURRENT DISTRIBUTION; DIOSCOREA; DIOSGENIN; FATTY ACIDS; MEDICINE, CHINESE TRADITIONAL; MOLECULAR STRUCTURE; PLANT EXTRACTS; PLANTS, MEDICINAL;

EID: 58749104344     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.200800295     Document Type: Article

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