Purification and Wnt-inhibitory activities of secreted frizzled-related proteins

Methods in Molecular Biology

Volumn 468, Issue , 2008, Pages 31-44

  Wolf, Vladimir ^a   Endo, Yoshimi ^a   Rubin, Jeffrey S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Chelat ing chromatography; Heparin chromatography; Protein purification; Recombinant expression; SFRP; Wnt

Indexed keywords

MAMMALIA;

FRIZZLED RELATED PROTEIN-1; MEMBRANE PROTEIN; PROTEIN; RECOMBINANT PROTEIN; SECRETED FRIZZLED RELATED PROTEIN 1; SFRP2 PROTEIN, HUMAN; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; CELL LINE; CHROMATOGRAPHY; CULTURE MEDIUM; HUMAN; INSTRUMENTATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ANIMALS; CELL LINE; CHROMATOGRAPHY; CULTURE MEDIA, CONDITIONED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MEMBRANE PROTEINS; PROTEINS; RECOMBINANT PROTEINS;

EID: 58749101212     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-249-6_3     Document Type: Article

References (13)

1. Kawano, Y. and Kypta, R. (2003) Secreted antagonists of the Wnt signalling pathway. J. Cell Sci. 116, 2627-2634.
2. Rubin, J.S., Barshishat-Kupper, M., Feroze-Merzoug, F., and Xi, Z.F. (2006) Secreted WNT antagonists as tumor suppressors: pro and con. Front. Biosci. 11, 2093-2105.
3. Lee, H.X., Ambrosio, A.L., Reversade, B., and De Robertis, E.M. (2006) Embryonic dorsal-ventral signaling: secreted frizzled-related proteins as inhibitors of tolloid proteinases. Cell 124, 147-159.
4. Rodriguez, J., Esteve, P., Weinl, C., Ruiz, J.M., Fermin, Y., Trousse, F., et al. (2005) SFRP1 regulates the growth of retinal ganglion cell axons through the Fz2 receptor. Nat. Neurosci. 8, 1301-1309.
5. Hoang, B., Moos, M., Vukicevic, S., and Luvten, F.P. (1996) Primary structure and tissue distribution of Frzb, a novel protein related to Drosophila frizzled, suggest a role in human skeletal morphogenesis. J. Biol. Chem. 271, 26131-26137.
6. Finch, P.W., He., X., Kelley, M.J., Uren, A., Schaudies, RP, Popescu, N.C., et al. (1997) Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action. Proc. Natl. Acad. Sci. U.S.A. 94, 6770-6775.
7. Roszmusz, E., Patthy, A., Trexler, M., and Patthy, L. (2001) Localization of disulfide bonds in the Frizzled module of Rorl receptor tyrosine kinase. J. Biol. Chem. 276, 18485-18490.
8. Üren, A., Rekhsman, F., Anest, V., Taylor, WG., Muraiso, IC, Bottaro, D.P., et al. (2000) Secreted Frizzled-related protein-1 binds directly to Wingless and is a biphasic modulator of Wnt signaling. J. Biol. Chem. 275, 4374-4382.
9. Dann, C.E., Hsieh, J.-C., Rattner, A., Sharma, D., Nathans, J., and Leahy, D.J. (2001) Insights into Wnt binding and signalling from the structures of two Frizzled cysteine-rich domains. Nature 412, 86-90.
10. Levashova, Z.B., Plisov, S.Y, and Perantoni, A.O. (2003) Conditionally immortalized cell line of inducible metanephric mesenchyme. Kidney Int. 63, 2075-2087.
11. Endo, Y., Wolf, V., Muraiso, K., Kamijo, IC, Soon, L., Üren, A., et al. (2005) Wnt3a-dependent cell motility involves RhoA activation and is specifically regulated by Dishevelled-2. J Biol. Chem. 280, 777-786.
12. Bafico, A., Gazit,A., Pramila, T., Finch, PW, Yaniv, A., and Aaronson, SA. (1999) Interaction of Frizzled-related protein (FRP) with Wnt ligands and the Frizzled receptor suggests alternative mechanisms for FRP inhibition of Wnt signaling. J. Biol. Chem. 274, 16180-16187.
13. Gonzales-Sancho, J.M., Brennan, ICR, CasteloSoccio, LA., and Brown, A.M.C (2004) Wnt proteins induce Dishevelled phosphorylation via an LRP5/6-independent mechanism, irrespective of their ability to stabilize b-catenin. MoL. Cell. Biol. 24, 4757-4768.