Production of polyclonal antisera

Current Protocols in Cytometry

Volumn , Issue SUPPL. 43, 2008, Pages

  Cooper, Helen M ^a   Paterson, Yvonne ^b  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Adjuvant; Antigen; Immunization; Polyclonal antiserum

Indexed keywords

ANTIGEN; BOVINE SERUM ALBUMIN; FREUND ADJUVANT; IMMUNOGLOBULIN; KEYHOLE LIMPET HEMOCYANIN; POLYCLONAL ANTIBODY;

ANTIBODY PRODUCTION; BIOCHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION PROFILING; HAMSTER; IMMUNIZATION; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; MEDICAL RESEARCH; MOLECULAR BIOLOGY; MOUSE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEOMICS; RABBIT; RAT; REVIEW;

ANIMALIA; CRICETINAE; MUS; ORYCTOLAGUS CUNICULUS; RATTUS;

EID: 58749088783     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cya03is43     Document Type: Review

References (23)

1. Aguilar, J.C. and Rodríguez, E.G. 2007. Vaccine adjuvants revisited. Vaccine 25:3752-3762.
2. Benjamin, D.C., Berzofsky, J.A., East, I.J., Gurd, F.R.N., Hannum, C., Leach, S.J., Margoliash, E., Michael, J.G., Miller, A., Prager, E.M., Reichlin, M., Sercarz, E.E., Smith-Gill, S.J., Todd, P.E., and Wilson, A.C. 1984. The antigenic structure of proteins: A reappraisal. Annu. Rev. Immunol. 2:67-101.
3. Bennett, B., Check, I.J., Olsen, M.R., and Hunter, R.L. 1992.Acomparison of commercially available adjuvants for use in research. J. Immunol. Methods 153:31-40.
4. Bonifacino, J.S., Dell'Angelica, E.C., and Springer, T.A. 1999. Immunoprecipitation. Curr. Protoc. Mol. Biol. 48:10.16.1-10.16.29.
5. Deeb, B.J., DiGiacomo, R.F., Kunz, L.L., and Stewart, J.L. 1992. Comparison of Freund's and Ribi adjuvants for inducing antibodies to the synthetic antigen (TG)-AL in rabbits. J. Immunol. Methods 152:105-113.
6. Donovan, J. and Brown, P. 2006a. Parenteral injections. Curr. Protoc. Immunol. 73:1.6.1-1.6.10.
7. Donovan, J. and Brown, P. 2006b. Blood collection. Curr. Protoc. Immunol. 73:1.7.1-1.7.9.
8. Donovan, J. and Brown, P. 2007a.Animal health assurance. Curr. Protoc. Immunol. 76:1.1.1-1.1.3.
9. Donovan, J. and Brown, P. 2007b. Managing immunocompromised animals. Curr. Protoc. Immunol. 77:1.2.1-1.2.5.
10. Freund, J., Casals, J., and Hismer, E.P. 1937. Sensitization and antibody formation after injection of tubercle bacilli and paraffin oil. Proc. Soc. Exp. Biol. Med. 37:509.
11. Gallagher, S. 2006. One-dimensional SDS gel electrophoresis of proteins. Curr. Protoc. Mol. Biol. 75:10.2A.1-10.2A.37.
12. Gallagher, S., Winston, S.E., Fuller, S.A., Hurrell, J.G.R. 2004. Immunoblotting and immunodetection. Curr. Protoc. Mol. Biol. 66:10.8.1-10.8.24.
13. Grant, G.A. 2002. Synthetic peptides for production of antibodies that recognize intact proteins. Curr. Protoc. Mol. Biol. 59:11.16.1-11.16.19.
14. Hornbeck, P. 1991. Enzyme-linked immunosorbent assays. Curr. Protoc. Immunol. 1:2.1.1-2.1.22.
15. Johnston, B.A., Eisen, H., and Fry, D. 1991. An evaluation of several adjuvant emulsion regimens for the production of polyclonal antisera in rabbits. Lab Anim. Sci. 41:15-21.
16. Klinman, N.R. and Press, J. 1975. The B cell specificity repertoire: Its relationship to definable subpopulations. Transplant. Rev. 24:41-83.
17. Koh, Y.T., Higgins, S.A., Weber, J.S., and Kast, W.M. 2006. Immunological consequences of using three different clinical/laboratory techniques of emulsifying peptide-based vaccines in incomplete Freund's adjuvant. J. Transl. Med. 4:42-54.
18. Larsson, K., Wester, K., Nilsson, P., Uhlén, M., Hober, S., and Wernérus, H. 2006. Multiplexed PrEST immunization for high-throughput affinity proteomics. J. Immunol. Meth. 315:110-120.
19. Liang, M.T., Davies, N.M., Blanchfield, J.T., and Toth, I. 2006. Particulate systems as adjuvants and carriers for peptide and protein antigens. Curr. Drug Deliv. 3:379-388.
20. McWilliam, A. and Niemi, S.M. 1988. Freund's adjuvant. Canadian Council on Animal Care Resource 12:1.
21. Sasse, J. and Gallagher, S.R. 2003. Staining proteins in gels. Curr. Protoc. Mol. Biol. 63:10.6.1-10.6.25.
22. Smith, D.E., O'Brien, M.E., Palmer, V.J., and Sadowski, J.A. 1992. The selection of an adjuvant emulsion for polyclonal antibody production using a low-molecular-weight antigen in rabbits. Lab. Anim. Sci. 42:599-601.
23. Yokoyama, W.M. 2000. Monoclonal antibody supernatant and ascites fluid production. Curr. Protoc. Immunol. 40:2.6.1-2.6.9.