Developing a simple method to process bone samples prior to DNA isolation

Legal Medicine

Volumn 11, Issue 2, 2009, Pages 76-79

  Li, Richard ^a   Chapman, Sandra ^b   Thompson, Mary ^b   Schwartz, Michal ^b  

^a JOHN JAY COLLEGE OF CRIMINAL JUSTICE   (United States)

^b SAM HOUSTON STATE UNIVERSITY   (United States)

Author keywords

Bone; DNA; Forensic; Trypsin

Indexed keywords

ANIMALS; DNA; FEMUR; FORENSIC MEDICINE; HYDROGEN-ION CONCENTRATION; MICROSCOPY, ELECTRON, SCANNING; ORGAN PRESERVATION SOLUTIONS; SPECIMEN HANDLING; SUS SCROFA; TEMPERATURE; TRYPSIN;

DNA; PRESERVATION SOLUTION; TRYPSIN;

ANIMAL; CHEMISTRY; FEMUR; FORENSIC MEDICINE; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METABOLISM; PH; PROCEDURES; SCANNING ELECTRON MICROSCOPY; SWINE; TEMPERATURE;

EID: 58749085537     PISSN: 13446223     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.legalmed.2008.09.003     Document Type: Article

References (9)

1. Lawler A. Terrorism massive DNA identification effort gets under way. Science 294 (2001) 278-279
2. Buck F.F., Vithayathil A.J., Bier M., and Nord F.F. On the mechanism of enzyme action. 73. Studies on trypsins from beef, sheep and pig pancreas. Arch Biochem Biophys 97 (1962) 417-424
3. Walsh K. Trypsinogens and trypsins of various species. Meth Enzymol 19 (1970) 41-63
4. Hangay G., and Dingley M. Biological museum methods (1985), Academic Press, Orlando
5. Hendry D. Vertebrates. In: Carter D., and Walker A.K. (Eds). Care and conservation of natural history collections (1999), Butterworth-Heinemann, Oxford 1-36
6. Caragine T., Sebestryen J., Badja E., Shaler R., and Prinz M. Methodologies to employ porcine tissue to simulate human tissue: determining the efficacy of radiation for decontamination and the subsequent recovery of DNA from tissue for PCR DNA testing. Proc Am Acad Forensic Sci 11 (2005) 66
7. Kunitz M. Crystalline soybean trypsin inhibitor II. General properties. J Gen Physiol 30 (1947) 291-310
8. Letourneau F., Soussotte V., Bressollier P., Branland P., and Verneuil B. Keratinolytic activity of Streptomyces sp. S.K1-02: a new isolated strain. Lett Appl Microbiol 26 (1998) 77-80
9. Isenberg A. Forensic mitochondrial DNA analysis. In: Saferstein R. (Ed). Forensic science handbook, vol. II. 2nd ed. (2005), Pearson, Upper Saddle River, NJ 297-327