Novel NGF-potentiating diterpenoids from a Brazilian medicinal plant, Ptychopetalum olacoides

Bioorganic and Medicinal Chemistry Letters

Volumn 19, Issue 3, 2009, Pages 882-886

  Tang, Wanxia ^a   Kubo, Miwa ^a   Harada, Kenichi ^a   Hioki, Hideaki ^a   Fukuyama, Yoshiyasu ^a  

^a TOKUSHIMA BUNRI UNIVERSITY   (Japan)

Author keywords

Clerodane type; Diterpenoid; Neurite outgrowth promoting activity; NGF mediated PC12 cells; Olacaceae; Ptychopetalum olacoides

Indexed keywords

6ALPHA,7ALPHA DIHYDROXYANNONENE; 7ALPHA HYDROXYSOLIDAGOLACTONE I; 7ALPHA,20 DIHYDROXYANNONENE; CLERODANE DERIVATIVE; NERVE GROWTH FACTOR; PTYCHO 6ALPHA,7ALPHA DIOL; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG EFFECT; DRUG ISOLATION; DRUG POTENTIATION; DRUG STRUCTURE; MEDICINAL PLANT; NERVE FIBER GROWTH; NONHUMAN; PTYCHOPETALUM OLACOIDES; RAT; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

ANIMALS; BIOLOGICAL ASSAY; CELL DIFFERENTIATION; DITERPENES; DITERPENES, CLERODANE; DRUG SYNERGISM; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR CONFORMATION; NERVE GROWTH FACTOR; NEURITES; OLACACEAE; PC12 CELLS; PLANTS, MEDICINAL; RATS; SPECTROPHOTOMETRY, INFRARED;

OLACACEAE; PTYCHOPETALUM OLACOIDES;

EID: 58549112940     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2008.11.100     Document Type: Article

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32. 2 for 24 h. The culture medium was then changed to DMEM, 2% HS, 1% FBS, and 100 IU/mL penicillin and 100 μg/mL streptomycin. At the same time, different concentrations of test samples with or without NGF were added. One concentration experiment was repeated in three wells. After incubation with samples for 4 days, the cultures were fixed with 4% paraformaldehyde/PBS, and stained with methylene blue. Cell morphology was observed under a phase-contrast microscope and neurite length was quantified. Five images were selected randomly under microscope for each well and five cells bearing well-developed neurite in each photo were selected and the average length of the longest neurite on selected cells was measured. At least 75 cells were calculated for each concentration. Statistical analyses were performed using Dunnett's t-test. Compound 1 showed significant neurite outgrowth-promoting activity on 20 ng/mL NGF-mediated PC12 cells at the concentrations ranging from 0.1 to 50.0 μM (Figs. 3 and 4). Compound 2 also promoted the neurite length of 20 ng/mL NGF-mediated PC12 cells at the concentrations ranging from 0.1 to 30.0 μM (Figs. 3 and 4) and showed cell toxicity at 50.0 μM. Compounds 3 and 4, and derivative 1a showed no activity on 20 ng/mL NGF-potentiating PC12 cells at the concentrations ranging from 0.1 to 50.0 μM, respectively.