Protein fractionation according to molecular size in constant pH media with immobilized charges in colinear porosity gradient

Analytical Biochemistry

Volumn 386, Issue 1, 2009, Pages 116-118

  Stoyanov, Alexander V ^a,b  

^a CHILDREN S HOSPITAL   (United States)

^b USA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTRIC FIELDS; ELECTROPHORESIS; POROSITY; SEPARATION;

COMPLEX CONDUCTIVITY; ELECTROPHORETIC SEPARATIONS; GEL CONCENTRATION; NONUNIFORM ELECTRIC FIELD; POROSITY GRADIENTS; PROTEIN ELECTROPHORESIS; PROTEIN FRACTIONATION; PROTEIN SEPARATIONS;

PROTEINS;

BUFFER; DETERGENT; PROTEIN;

ANALYTIC METHOD; ARTICLE; CONCENTRATION (PARAMETERS); CONDUCTANCE; ELECTRIC FIELD; MOLECULAR SIZE; MOLECULAR WEIGHT; PH; POROSITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ELECTROPHORESIS; PROTEIN IMMOBILIZATION;

EID: 58549110299     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.12.001     Document Type: Article

References (16)

1. Mosher R., Saville D., and Thormann W. The Dynamics of Electrophoresis (1990), VCH, Weinheim
2. Stoyanov A.V., and Righetti P.G. Steady-state electrolysis of a solution of non-amphotheric compounds. Electrophoresis 20 (1999) 718-722
3. Stoyanov A.V., and Pawliszyn J. Buffer composition changes in background electrolyte during electrophoretic run in capillary zone electrophoresis. Analyst 129 (2004) 979-984
4. A. Rosengren, B. Bjellqvist, V. Gasparic, US patent 4130470, 1978.
5. Bjellqvist B., Ek K., Righetti P.G., Gianazza E., Gorg A., Westermeier R., Postel W.J., and principle I.i.i.p.g.:. methodology and some applications. Biochem. Biophys. Methods 6 (1982) 317-339
6. Righetti P.G., Stoyanov A.V., and Zhukov M.Y. Proteome Revisited: Theory and Practice of All Relevant Electrophoretic Steps (2001), Elsevier, Amsterdam
7. Stoyanov A.V., and Righetti P.G. Dynamics of protein isoelectric focusing in immobilized pH gradient gels. Electrophoresis 17 (1996) 1313-1318
8. Chrambach A. The Practice of Quantitative Gel Electrophoresis (1985), VCH, Verlagsgesellschaft, Weinheim
9. A. Nguyen, M. Moini, Analysis of major protein-protein and protein-metal complexes of erythrocytes directly from cell lysate utilizing capillary electrophoresis mass spectrometry, Anal. Chem. 10.1021/ac801158q.
10. Shihabi Z., and Hinsdale M. Simplified hemoglobin chain detection by capillary electrophoresis. Electrophoresis 26 (2005) 581-585
11. Schaeffer J.R., McDonald M.J., Turci S.M., Dinda D.M., and Dimer-monomer dissociation of human hemoglobin H.Bunn. J. Biol. Chem. (1984)
12. Westermeier R. Electrophoresis in Practice (2005), Weinheim, Willey-VCH
13. Toda T., Fujita T., and Onashi M. Two-dimensional electrophoresis on the layers of acetate membrane. Anal. Biochem. 119 (1982) 167-176
14. Eley M.N., Burns P.C., Kannapel C.C., and Campbell P.S. Cetyltrimethylammonium bromide polyacrylamide gel electrophoresis: estimation of protein subunit molecular weights using cationic detergents. Anal. Biochem. 92 (1979) 411-419
15. Swaney J.B., Woude G.V.F., and Bachrach H.L. Sodium dodecyl sulfate-dependent anomalies in gel electrophoresis. Anal. Biochem. 58 (1974) 337-346
16. Dohnal J.C., and Garvin J.E. The interaction of dodecyl and tetradecyl sulfate with proteins during polyacrylamide gel electrophoresis. Biochim. Biophys. Acta 576 (1979) 393-403