Detection of shiga-toxigenic Escherichia coli (STEC) in diarrhoeagenic stool & meat samples in Mangalore, India

Indian Journal of Medical Research

Volumn 128, Issue 3, 2008, Pages 271-277

  Dhanashree, B ^a   Shrikar Mallya, P ^a  

^a KASTURBA MEDICAL COLLEGE   (India)

Author keywords

Antibiotic resistance; Bovine meat; Eae positive E. coli; PCR; Shiga toxigenic Escherichia coli; Stool

Indexed keywords

AMPICILLIN; BACTERIAL PROTEIN; CEFOTAXIME; CEFOXITIN; CEFTAZIDIME; CEFTRIAXONE; CEFUROXIME; CIPROFLOXACIN; GENTAMICIN; PROTEIN BFP; PROTEIN EAE; PROTEIN HLYA; PROTEIN RFB; PROTEIN STX1; PROTEIN STX2; UNCLASSIFIED DRUG;

ADOLESCENT; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; BEEF; CHILD; CONTROLLED STUDY; CULTURE MEDIUM; DIARRHEA; ESCHERICHIA COLI; FECES CULTURE; FOOD ANALYSIS; FOOD CONTAMINATION; GENE IDENTIFICATION; HUMAN; INDIA; INFANT; MAJOR CLINICAL STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; SEROTYPING; SHIGA TOXIN PRODUCING ESCHERICHIA COLI;

ADHESINS, BACTERIAL; ADOLESCENT; BACTERIAL TOXINS; CHILD; CHILD, PRESCHOOL; DIARRHEA; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FECES; FOOD CONTAMINATION; HUMANS; INCIDENCE; INDIA; INFANT; MEAT; SHIGA TOXIN 1; SHIGA TOXIN 2; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 58549107859     PISSN: 09715916     EISSN: 09715916     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev 1998; 11: 142-201.
2. Clarke SC, Haigh RD, Freestone PE, Williams PH. Virulence of enteropathogenic Escherichia coli, a global pathogen. Clin Microbiol Rev 2003; 16: 365-78.
3. Nunes BE, Saridakis OH, Irino K. Genotypic and phenotypic characterization of attaching and effacing Escherichia coli (AEEC) isolated from children with and without diarrhoea Londrina, Brazil. J Med Microbiol 2003; 52: 499-504.
4. Trabulsi LR, Keller R, Gomesn TA. Typical and atypical enteropathogenic Escherichia coli. Emerg Infect Dis 2002; 8: 508-13.
5. Paton JC, Paton AW. Pathogenesis and diagnosis of shiga toxin-producing Escherichia coli infections. Clin Microbiol Rev 1998; 11: 450-79.
6. Beutin L, Geier D, Steinruck H, Zimmermann S, Scheutz F. Prevalence and some properties of verotoxin (shiga-like toxin) producing Escherichia coli in seven different species of healthy domestic animals. J Clin Microbiol 1993; 31: 2483-8.
7. Elliott EJ, Robins- browne RM, O'Loughlin EV, Bennett-Wood V, Bourke J, Henning P, et al. Nation wide study of haemolytic uremic syndrome: clinical microbiological and epidemiological features. Arch Dis Child 2001; 85: 125-31.
8. Khan A, Yamasaki S, Sato T, Ramamurthy T, Pal A, Datta S, et al. Prevalence and genetic profiling of virulence determinants of Non-O157 shiga toxin producing Escherichia coli isolated from cattle, beef and humans, Calcutta, India. Emerg Infect Dis 2002; 8: 54-62.
9. Kumar HS, Otta SK, Karunasagar I, Karunasagar I. Detection of Shiga-toxigenic Escherichia coli (STEC) in fresh seafood and meat marketed in Mangalore, India by PCR. Lett Appl Microbiol 2001; 33: 334-8.
10. De Boer E, Heuvelink AE. Methods for the detection and isolation of shiga toxin producing Escherichia coli. J Appl Microbiol 2000; 88: S133-43.
11. Collee JG, Miles RS, Watt B. Tests for the identification of bacteria. In: Collee JG, Fraser AG, Marmion BP, Simmons A, editors. Mackie & McCartney practical medical microbiology, 14th ed. London: Churchill Livingstone; 1996. p. 131-49.
12. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standard single disk method. Am J Clin Pathol 1966; 45: 493-6.
13. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial disk susceptibility tests. 8th ed. CLSI Approved standard M02-A8. Wayne, Pa. 2005.
14. Makino S, Okada Y, Maruyama T. A new method for direct detection of Listeria monocytogenes from food by PCR. Appl Environ Microbiol 1995; 61: 3745-7.
15. Paton AW, Paton JC. Detection and characterization of shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohaemorrhagic E. coli hlyA, rfbO111 and rfbO157. J Clin Microbiol 1998; 36: 598-602.
16. Fagan PK, Hornitzky MA, Bettelheim KA, Djordjevic SP. Detection of Shiga like toxin (stx1 and stx2), Intimin (eaeA) and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR. Appl Environ Microbiol 1999; 65: 868-72.
17. Desmarhelier PM, Bilge SS, Fegan N, Mills L, Vary JC Jr, Tarr PI. A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide. J Clin Microbiol 1998; 36: 1801-4.
18. Fratamico PM, Sackitey SK, Wiedmann M, Deng MY. Detection of Escherichia coli O157:H7 by multiplex PCR. J Clin Microbiol 1995; 33: 2188-91.
19. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual. New York: Cold Spring Harbor Laboratory Press; 1989.
20. Yatsuyanagi J, Saito S, Miyajima Y, Amano K, Enomoto K. Characterization of typical enteropathogenic Escherichia coli strains harboring the astA gene that were associated with a waterborne outbreak of diarrhea in Japan. J Clin Microbiol 2003; 41: 2033-9.
21. Vieira MA, Andrade JR, Trabulsi LR, Rosa AC, Dias AM, Ramos SR, et al. Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E. coli (EPEC) serogroups that carry eae and lack the EPEC adherence factor and shiga toxin DNA probe sequences. J Infect Dis 2001; 183: 762-72.
22. Afset JE, Bevanger L, Romundstad P, Bergh K. Association of atypical enteropathogenic Escherichia coli (EPEC) with prolonged diarrhea. J Med Microbiol 2004; 53: 1137-44.
23. Schmidt H, Plaschke B, Franke S, Russmann H, Schwarzkopf A, Heesemann J, et al. Differentiation in virulence patterns of Escherichia coli possessing eae genes. Med Microbiol Immunol 1995; 183: 23-31.
24. Baba A, Ebuchi S, Uryu K, Hiwaki H, Ogata K, Washimi E, et al. An outbreak of water borne gastroenteritis caused by diarrheagenic Escherichia coli possessing eae gene. Jpn J Infect Dis 2006; 59: 59-60.
25. Schroeder CM, Meng J, Zhao S, DebRoy C, Torcolini J, Zhao C, et al. Antimicrobial resistance of Escherichia coli O26, O103, O111, O128 and O145 from animals and humans. Emerg Infect Dis 2002; 8: 1409-14.
26. Bettelheim KA, Hornitzky MA, Djordjevic SP, Kuzevski A. Antibiotic resistance among verocytotoxigenic Escherichia coli (VTEC) and non-VTEC isolated from domestic animals and humans. J Med Microbiol 2003; 52: 155-62.
27. Bettelheim KA. The VTEC table. In: Serotypes of VTEC. [updated May 2003; last accessed 2007 May 15]. Available from: http://www.microbionet.com.au/vtectable.htm