Activity of phosphatases and esterases in the aphid, Acyrthosiphon pisum (Hemiptera: Sternorrhyncha: Aphididae), and in the gut wall of Galleria mellonella (Lepidoptera: Pyralidae) larvae and pupae

European Journal of Entomology

Volumn 93, Issue 1, 1996, Pages 37-44

  Némec, Václav ^a   Ẑenka, Jan ^b  

^a INSTITUTE OF ENTOMOLOGY   (Czech Republic)

^b NONE   (Czech Republic)

Author keywords

Acyrthosiphon pisum; Ecdysone titre; Ecdysteroid mobilization; Enzyme kinetics; Esterase; Galleria mellonella; Phosphatases; Zymogramme patterns

Indexed keywords

ACYRTHOSIPHON PISUM; APHIDIDAE; GALLERIA MELLONELLA; HEMIPTERA; INSECTA; LEPIDOPTERA; PISUM; PYRALIDAE; STERNORRHYNCHA;

EID: 5844262986     PISSN: 12105759     EISSN: 18028829     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. AIDELLS B., LOCKSHIN R.A. & CULLIN A.M. 1971: Breakdown of the silk glands of Galleria mellonella: acid phosphatases in involving glands. J. Insect Physiol. 17: 857-869.
2. CONNAT J.L. & DIEHL P.A. 1986: Probable occurence of ecdysteroid fatty acid esters in different classes of Arthropods. Insect Biochem. 16: 91-97.
3. DINAN L.N. & REES H.H. 1981: The identification and titres of conjugated and free ecdysteroids in developing ovaries and newly-laid eggs of Schistocerca gregaria. J. Insect Physiol. 27: 51-57.
4. 2H-ecdysone in Schistocerca gregaria: formation of ecdysteroid acids together with free and phosphorylated ecdysteroid acetates. Arch. Insect Biochem. Physiol. 1: 385-407.
5. GOA J. 1953: A microbiuret method for protein determination. Scand. J. Clin. Lab. Invest. 5: 218-222.
6. GRAU V. & LAFONT R. 1994: The distribution of ecdysone metabolites within the body of adult Drosophila melanogaster females and their sites of production. J. Insect Physiol. 40: 87-96.
7. GREBENOK R.J., VENKATACHARI S. & ADLER J.H. 1994: Biosynthesis of ecdysone and ecdysone phosphates in spinach, Spinacia oleracea. Phytochemistry 36: 1399-1408.
8. HANES C.S. 1932: The effect of starch concentration upon velocity of hydrolysis by the amylase of germinated barley. Biochem. J. 26: 1406-1421.
9. HOFFMANN J.A. & LAGUEUX M. 1985: Endocrine aspects of embryonic development in Insects. In Kerkut G.A. & Gilbert L.I. (eds): Comprehensive Insect Physiology, Biochemistry and Pharmacology. Vol. 1. Pergamon Press, Oxford, pp. 435-460.
10. HUGGINS C. & LAPIDES J. 1947: IV. Acyl esters of para-nitrophenol as substrates for the colorimetric determination of esterases. J. Biol. Chem. 170: 467-482.
11. ISAAC R.E., SWEENEY F.P. & REES H.H. 1983: Enzymatic hydrolysis of ecdysteroid phosphate during embryogenesis in the desert locust (Schistocerca gregaria). Biochem. Soc. Trans. 11: 379-380.
12. KOOLMAN J. & KARLSON P. 1985: Regulation of ecdysteroid titer: degradation. In Kerkut G.A. & Gilbert L.I. (eds): Comprehensive Insect Physiology, Biochemistry and Pharmacology. Vol. 7. Pergamon Press, Oxford, pp. 343-361.
13. LAFONT R. & CONNAT J.L. 1989: Pathways of ecdysone metabolism. In Koolman J. (ed.): Ecdysone. Georg Thieme Verlag, Stuttgart, pp. 167-173.
14. LAMBREMONT E.N. 1960: Post-emergence change of enzyme activity in the mosquito, Aedes aegypti (L.). Ann. Entomol. Soc. Am. 53: 86-91.
15. LINEWEAVER H. & BURK D. 1934: The determination of enzyme dissociation constants. J. Am. Chem. Soc. 56: 658-666.
16. LINHARDT K. & WALTER K. 1965: Phosphatases. In Bergmayer H.U. (ed.): Methods of Enzymatic Analysis. Academic Press, New York, pp. 146-150.
17. LOCKSHIN R.A. & WILLIAMS C.M. 1965: Programmed cell death. V. Cytosolic enzymes in relation to the breakdown of intersegmental muscles of silkmoths. J. Insect Physiol. 11: 831-844.
18. LOXDALE H.D. 1983: Electrophoretic study of enzymes from cereal aphid populations. I. Electrophoretic techniques and staining system for characterising isozymes from six species of cereal aphids (Homoptera: Aphididae). Bull. Entomol. Res. 73: 645-657.
19. MODDE J.F., LAFONT R. & HOFFMANN J.A. 1984: Ecdysone metabolism in Locusta migratoria larvae and adults. Int. J. Invert. Reprod. Dev. 7: 161-183.
20. MORIBAYASHI A. & OHTAKI T. 1980: Inactivation and reactivation of 20-hydroxyecdysone during pupaladult development of the flesh fly, Sarcophaga peregrina. Jap. J. Med. Sci. Biol. 33: 189-201.
21. NÉMEC V. 1972: The effects of a juvenile hormone analogue on the esterase activity in the haemolymph of Pyrrhocoris apterus (L.). Acta Entomol. Bohemoslov. 69: 137-142.
22. NÉMEC V. & SOCHA R. 1988: Phosphatases and pteridines in Malpighian tubules: a possible marker of the mosaic mutant in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae). Acta Entomol. Bohemoslov. 85: 321-326.
23. REES H.H. 1995: Ecdysteroid biosynthesis and inactivation in relation to function. Eur. J. Entomol. 92: 9-39.
24. REES H.H. & ISAAC R.E. 1984: Biosynthesis of ovarian ecdysteroid phosphates and their metabolic fate during embryogenesis in Schistocerca gregaria. In Hoffmann J. & Porchet M. (eds): Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones. Springer Verlag, Berlin, pp. 181-195.
25. REES H.H. & ISAAC R.E. 1985: Biosynthesis and metabolism of ecdysteroids and methods of isolation and identification of the free and conjugated compounds. Meth. Enzymol. 111: 377-400.
26. SALL C., TSOUPRAS G., LAGEAUX M., ZACHARY D., LUU B. & HOFFMANN J.A. 1983: Fate of maternal conjugated ecdysteroids during embryonic development in Locusta migratoria. J. Insect Physiol. 29: 491-507.
27. SCALIA S., SBRENNA-MIACCIARELLI A., SBRENNA G. & MORGAN E.D. 1987: Edcysteroid titres and location in developing eggs of Schistocerca gregaria. Insect Biochem. 17: 227-236.
28. SEHNAL F. 1966: Kritisches Studium der Bionomie und Biometrik der in verschiedenen Lebensbedingungen gezuchteten Wachsmotte, Galleria mellonella (L.) (Lepidoptera). Z. Wiss. Zool. 174: 53-82.
29. SEHNAL F. & ŽITṄAN D. 1990: Endocrines of insect gut. In Epple A., Scanes C.G. & Stetson-M.H. (eds): Progress in Comparative Endocrinology. Wiley-Liss Inc., New York, pp. 510-515.
30. SEHNAL F., DELBECQUE J.P., MAROY P. & MALÁ J. 1986: Ecdysteroid titres during larval life and metamorphosis. Insect Biochem. 86: 157-162.
31. SLINGER A.J. & ISAAC R.E. 1988a: Synthesis of apolar ecdysteroid conjugates by ovarian tissue from Periplaneta americana. Gen. Comp. Endocrinol. 70: 74-82.
32. SLINGER A.J. & ISAAC R.E. 1988b: Ecdysteroid titres during embryogenesis in the cockroach, Periplaneta americana. J. Insect Physiol. 34: 1119-1125.
33. 14C-cholesterol. Arch. Insect Biochem Physiol. 7: 157-172.
34. WIGGLESWORTH V.B. 1965: The Principles of Insect Physiology. 5th ed. Methuen & Co., London, 741 pp.
35. WILLIAMS D.E. & REISFELD R.A. 1964: Disc electrophoresis in polyacrylamide gels: Extension to new conditions of pH and buffer. Ann. N.Y. Acad. Sci. 121: 373-381.
36. ŽENKA J. 1994: LABCHEM, a software programme. Copyright Jan Ženka, České Budějovice.