Enterohemorrhagic Escherichia coli effector EspL2 induces actin microfilament aggregation through annexin 2 activation

Cellular Microbiology

Volumn 11, Issue 2, 2009, Pages 337-350

  Miyahara, Akira ^a   Nakanishi, Noriko ^a   Ooka, Tadasuke ^b   Hayashi, Tetsuya ^b   Sugimoto, Nakaba ^a   Tobe, Toru ^a  

^a OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; F ACTIN; LIPOCORTIN 2; PROTEIN ESPL2; UNCLASSIFIED DRUG;

ACTIN FILAMENT; ACTIN POLYMERIZATION; ANIMAL CELL; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL TRANSLOCATION; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; CELL AGGREGATION; CONTROLLED STUDY; CYTOSKELETON; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ENZYME ACTIVATION; ENZYME ACTIVITY; EPITHELIUM CELL; HOST CELL; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN DEPLETION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN LOCALIZATION;

ANNEXIN A2; BACTERIAL ADHESION; CELL LINE; EPITHELIAL CELLS; ESCHERICHIA COLI O157; ESCHERICHIA COLI PROTEINS; GENE DELETION; HUMANS; MICROFILAMENTS; MICROSCOPY, ELECTRON, SCANNING; PROTEIN BINDING; VIRULENCE FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOPODA;

EID: 58349118729     PISSN: 14625814     EISSN: 14625822     Source Type: Journal    
DOI: 10.1111/j.1462-5822.2008.01256.x     Document Type: Article

References (27)

1. Buchrieser, C., Glaser, P., Rusniok, C., Nedjari, H., d'Hauteville, H., Kunst, F., et al. (2000) The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri. Mol Microbiol 38: 760-771.
2. Campellone, K.G., Robbins, D., and Leong, J.M. (2004) EspFU is a translocated EHEC effector that interacts with Tir and N-WASP and promotes Nck-independent actin assembly. Dev Cell 7: 217-228.
3. Caron, E., Crepin, V.F., Simpson, N., Knutton, S., Garmendia, J., and Frankel, G. (2006) Subversion of actin dynamics by EPEC and EHEC. Curr Opin Microbiol 9: 40-45.
4. Coburn, B., Sekirov, I., and Finlay, B.B. (2007) Type III secretion systems and disease. Clin Microbiol Rev 20: 535-549.
5. Dahan, S., Wiles, S., La Ragione, R.M., Best, A., Woodward, M.J., Stevens, M.P., et al. (2005) EspJ is a prophage-carried type III effector protein of attaching and effacing pathogens that modulates infection dynamics. Infect Immun 73: 679-686.
6. Datsenko, K.A., and Wanner, B.L. (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97: 6640-6645.
7. DeVinney, R., Stein, M., Reinscheid, D., Abe, A., Ruschkowski, S., and Finlay, B.B. (1999) Enterohemorrhagic Escherichia coli O157: H7 produces Tir, which is translocated to the host cell membrane but is not tyrosine phosphorylated. Infect Immun 67: 2389-2398.
8. Donnenberg, M.S., and Whittam, T.S. (2001) Pathogenesis and evolution of virulence in enteropathogenic and enterohemorrhagic Escherichia coli. J Clin Invest 107: 539-548.
9. Falsey, R.R., Marron, M.T., Gunaherath, G.M., Shirahatti, N., Mahadevan, D., Gunatilaka, A.A., and Whitesell, L. (2006) Actin microfilament aggregation induced by withaferin A is mediated by annexin II. Nat Chem Biol 2: 33-38.
10. Garmendia, J., Phillips, A.D., Carlier, M.F., Chong, Y., Schuller, S., Marches, O., et al. (2004) TccP is an enterohaemorrhagic Escherichia coli O157:H7 type III effector protein that couples Tir to the actin-cytoskeleton. Cell Microbiol 6: 1167-1183.
11. Gerke, V., Creutz, C.E., and Moss, S.E. (2005) Annexins: Linking Ca2+ signalling to membrane dynamics. Nat Rev Mol Cell Biol 6: 449-461.
12. Gruenheid, S., Sekirov, I., Thomas, N.A., Deng, W., O'Donnell, P., Goode, D., et al. (2004) Identification and characterization of NleA, a non-LEE-encoded type III translocated virulence factor of enterohaemorrhagic Escherichia coli O157: H7. Mol Microbiol 51: 1233-1249.
13. Hayashi, T., Makino, K., Ohnishi, M., Kurokawa, K., Ishii, K., Yokoyama, K., et al. (2001) Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res 8: 11-22.
14. Hayes, M.J., Rescher, U., Gerke, V., and Moss, S.E. (2004) Annexin-actin interactions. Traffic 5: 571-576.
15. Hayes, M.J., Shao, D., Bailly, M., and Moss, S.E. (2006) Regulation of actin dynamics by annexin 2. EMBO J 25: 1816-1826.
16. Kaper, J.B., Nataro, J.P., and Mobley, H.L. (2004) Pathogenic Escherichia coli. Nat Rev Microbiol 2: 123-140.
17. Kelly, M., Hart, E., Mundy, R., Marches, O., Wiles, S., Badea, L., et al. (2006) Essential role of the type III secretion system effector NleB in colonization of mice by Citrobacter rodentium. Infect Immun 74: 2328-2337.
18. Kenny, B., and Finlay, B.B. (1997) Intimin-dependent binding of enteropathogenic Escherichia coli to host cells triggers novel signaling events, including tyrosine phosphorylation of phospholipase C-gamma1. Infect Immun 65: 2528-2536.
19. Kenny, B., Ellis, S., Leard, A.D., Warawa, J., Mellor, H., and Jepson, M.A. (2002) Co-ordinate regulation of distinct host cell signalling pathways by multifunctional enteropathogenic Escherichia coli effector molecules. Mol Microbiol 44: 1095-1107.
20. Nataro, J.P.J., Seriwatana, A., Fasano, D.R., Maneval, L.D., Guers, F., Noriega, F., et al. (1995) Identification and cloning of a novel plasmid-encoded enterotoxin of enteroinvasive Escherichia coli and Shigella strains. Infect Immun 63: 4721-4728.
21. Ogura, Y., Ooka, T., Asadulghani, J., Terajima, J., Nougayrede, P., Kurokawa, K., et al. (2007) Extensive genomic diversity and selective conservation of virulence-determinants in enterohemorrhagic Escherichia coli strains of O157 and non-O157 serotypes. Genome Biol 8: R138.
22. Rendon, M.A., Saldana, Z., Erdem, A.L., Monteiro-Neto, V., Vazquez, A., Kaper, J.B., et al. (2007) Commensal and pathogenic Escherichia coli use a common pilus adherence factor for epithelial cell colonization. Proc Natl Acad Sci USA 104: 10637-10642.
23. Rescher, U., Ruhe, D., Ludwig, C., Zobiack, N., and Gerke, V. (2004) Annexin 2 is a phosphatidylinositol (4,5)-bisphosphate binding protein recruited to actin assembly sites at cellular membranes. J Cell Sci 117: 3473-3480.
24. Tatsuno, I., Kimura, H., Okutani, A., Kanamaru, K., Abe, H., Nagai, S., et al. (2000) Isolation and characterization of mini-Tn5Km2 insertion mutants of enterohemorrhagic Escherichia coli O157:H7 deficient in adherence to Caco-2 cells. Infect Immun 68: 5943-5952.
25. Tobe, T., Beatson, S.A., Taniguchi, H., Abe, H., Bailey, C.M., Fivian, A., et al. (2006) An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination. Proc Natl Acad Sci USA 103: 14941-14946.
26. Tu, X., Nisan, I., Yona, C., Hanski, E., and Rosenshine, I. (2003) EspH, a new cytoskeleton-modulating effector of enterohaemorrhagic and enteropathogenic Escherichia coli. Mol Microbiol 47: 595-606.
27. Zobiack, N., Rescher, U., Laarmann, S., Michgehl, S., Schmidt, M.A., and Gerke, V. (2002) Cell-surface attachment of pedestal-forming enteropathogenic E.coli induces a clustering of raft components and a recruitment of annexin 2. J Cell Sci 115: 91-98.