An observation chamber for studying temperature-dependent and drug-induced events in live neurons using fluorescence microscopy

Analytical Biochemistry

Volumn 386, Issue 1, 2009, Pages 105-112

  Cheng, Wen Yao ^a   Hsu, Wei Lun ^a   Cheng, Hui Hsuan ^a   Huang, Zu Han ^a   Chang, Yen Chung ^a  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

Author keywords

Chamber; Fluorescence microscopy; Live cell; Temperature

Indexed keywords

CELLS; CYTOLOGY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; NEURONS; PHOTOBLEACHING; TEMPERATURE;

CHAMBER; CONFOCAL MICROSCOPES; CONTINUOUS MONITORING; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; HIPPOCAMPAL NEURONS; LIVE CELL; TEMPERATURE DEPENDENT; TEMPERATURE-DEPENDENT CHANGES;

MOLECULAR BIOLOGY;

ALPHA TUBULIN;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BIOLOGICAL MONITORING; CONFOCAL MICROSCOPY; CYTOSKELETON; DENDRITIC SPINE; EPIFLUORESCENCE MICROSCOPE; EPIFLUORESCENCE MICROSCOPY; FEMALE; FLUORESCENCE MICROSCOPY; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; HIPPOCAMPUS; MICROTUBULE; NERVE CELL; NERVE CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; RAT; SPRAGUE DAWLEY RAT; TEMPERATURE;

RATTUS;

EID: 58349109465     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.12.004     Document Type: Article

References (19)

1. Rieder C.L., and Cole R.W. Perfusion chambers for high-resolution video light microscopic studies of vertebrate cell monolayers: Some considerations and a design. Methods Cell Biol. 56 (1998) 253-275
2. Toyotomi S., and Momose Y. Temperature-controlled perfusion apparatus for microscope using transparent conducting film heater. Am. J. Physiol. 256 (1989) C214-C217
3. Rieder C.L., and Cole R.W. Cold shock and the mammalian cell cycle. Cell cycle 1 (2002) 169-175
4. Fujita J. Cold shock response in mammalian cells. J. Mol. Microbiol. Biotechnol. 1 (1999) 243-255
5. Shephard R.J., and Shek P.N. Cold exposure and immune function. Can. J. Physiol. Pharmacol. 76 (1998) 828-836
6. Fuller B.J. Gene expression in response to low temperatures in mammalian cells: a review of current ideas. Cryo-Lett. 24 (2003) 95-102
7. Sonna L.A., Fujita J., Gaffin S.L., and Lilly C.M. Effects of heat and cold stress on mammalian gene expression. J. Appl. Physiol. 92 (2002) 1725-1742
8. Asztely F., Erdemli G., and Kullmann D.M. Extrasynaptic glutamate spillover in the hippocampus: dependence on temperature and the role of active glutamate uptake. Neuron 18 (1997) 281-293
9. Sabatini B.L., and Regehr W.G. Timing of neurotransmission at fast synapses in the mammalian brain. Nature 384 (1996) 170-172
10. Somero G.N. Proteins and temperature. Annu. Rev. Physiol. 57 (1995) 43-68
11. Huang Z.H., Cheng H.H., Wu H.I., Tseng S.H., and Chang Y.C. Studying the temperature-dependent events of live cells under confocal and epi-fluorescence microscopy using a solid-state heating/cooling system. In: Mendex-Vilas A., and Diaz J. (Eds). Modern Research and Educational Topics in Microscopy (2007), Formatex, Badajoz, Spain 183-189
12. Brewer G.J., Torricelli J.R., Evege E.K., and Price P.J. Optimized survival of hippocampal neurons in B27-supplemented neurobasal, a new serum-free medium combination. J. Neurosci. Res. 35 (1993) 567-576
13. McNally J.G. Quantitative FRAP in analysis of molecular binding dynamics in vivo. Methods Cell Biol. 85 (2008) 329-351
14. Alvarez V.A., and Sabatini B.L. Anatomical and physiological plasticity of dendritic spines. Annu. Rev. Neurosci. 30 (2007) 79-97
15. Bourne J.N., and Harris K.M. Balancing structure and function at hippocampal dendritic spines. Annu. Rev. Neurosci. 31 (2008) 47-67
16. H.H. Cheng, Z.H. Huang, W.H. Lin, W.Y. Chow, Y.C. Chang, Cold-induced exodus of postsynaptic proteins from dendritic spines, J. Neurosci. Res., in press, doi: 10.1002/jnr.21852.
17. Giepmans B.N., Adams S.R., Ellisman M.H., and Tsien R.Y. The fluorescent toolbox for assessing protein location and function. Science 312 (2006) 217-224
18. Guillaud L., Bosc C., Fourest-Lieuvin A., Denarier E., Pirollet F., Lafanechere L., and Job D. STOP proteins are responsible for the high degree of microtubule stabilization observed in neuronal cells. J. Cell Biol. 142 (1998) 167-179
19. Yan Y., and Broadie K. In vivo assay of presynaptic microtubule cytoskeleton dynamics in Drosophila. J. Neurosci. Methods 162 (2007) 198-205