The use of molecular beacons to directly measure bacterial mRNA abundances and transcript degradation

Journal of Microbiological Methods

Volumn 76, Issue 2, 2009, Pages 146-151

  Kuechenmeister, Lisa J ^a   Anderson, Kelsi L ^a   Morrison, John M ^a   Dunman, Paul M ^a  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Gram positive; Molecular beacon; mRNA abundance; mRNA degradation

Indexed keywords

BACTERIAL RNA; MESSENGER RNA; SINGLE STRANDED RNA;

ANIMAL CELL; ARTICLE; BACTERIUM; CONTROLLED STUDY; GENE AMPLIFICATION; GENE CONTROL; GENE EXPRESSION; GENE LIBRARY; GENETIC TRANSCRIPTION; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; METHODOLOGY; MOLECULAR BEACON; NONHUMAN; PATHOGENICITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA DEGRADATION; STAPHYLOCOCCUS AUREUS; TRANSPOSON;

BACTERIAL PROTEINS; DNA PROBES; FLUORESCENT DYES; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR PROBE TECHNIQUES; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; REVERSE TRANSCRIPTION; RNA STABILITY; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL PROTEIN A; STAPHYLOCOCCUS AUREUS; VIRULENCE FACTORS;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

EID: 58349091217     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.10.004     Document Type: Article

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