Structural characterization of the transmembrane segments of the mitochondrial oxoglutarate carrier (OGC) by NMR spectroscopy

Italian Journal of Biochemistry

Volumn 56, Issue 4, 2007, Pages 285-288

  Morelli, Maria Antonietta Castiglione ^a   Ostuni, Angela ^a   Armentano, Francesca ^a   Palmieri, Ferdinando ^b   Bisaccia, Faustino ^a  

^a UNIVERSITY OF BASILICATA   (Italy)

^b UNIVERSITY OF BARI   (Italy)

Author keywords

Mitochondrial carriers; Mitochondrial oxoglutarate carrier; NMR; Structure; Transmembrane segments

Indexed keywords

CARRIER PROTEIN; MITOCHONDRIAL PROTEIN; OXOGLUTARATE TRANSLOCATOR;

ANIMAL; CATTLE; CHEMICAL STRUCTURE; CHEMISTRY; CIRCULAR DICHROISM; COMPUTER SIMULATION; CONFERENCE PAPER; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN SECONDARY STRUCTURE;

ANIMALS; CATTLE; CIRCULAR DICHROISM; COMPUTER SIMULATION; MAGNETIC RESONANCE SPECTROSCOPY; MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIAL PROTEINS; MODELS, MOLECULAR; PROTEIN STRUCTURE, SECONDARY;

EID: 58249124481     PISSN: 00212938     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (27)

1. Palmieri, F., Quagliariello, E., Klingenberg, M. Kinetics and specificity of the oxoglutarate carrier in rat liver mitochondria. Eur. J. Biochem. 1972;29:408-16.
2. Palmieri, F. The mitochondrial transporter family (SLC25): physiological and pathological implications. Pflugers Arch.-Europ. J. Physiol. 2004; 447:689-709.
3. Saraste, M. and Walker, J.E. Internal sequence repeats and the path of polypeptide in mitochondrial ADP/ATP translocase. FEBS Lett. 1982;144:250- 254.
4. Pebay-Peyroula E., Dahout-Gonzalez C., Kahn R., Trezeguet V., Lauquin G.J., Brandolin G. Structure of mitochondrial ADP/ATP carrier in complex with carboxyatractyloside. Nature 2003;426:39-44.
5. Bamber, L., Harding, M., Monné, M., Slotboom, D.J., Kunji, R.S. The yeast mitochondrial ADP/ATP carrier functions as a monomer in mitochondrial membranes. Proc. Natl. Acad. Sci. USA 2007; 104: 10830-4.
6. Palmieri, F. and van Ommen, B. The mitochondrial carrier protein family. In: Frontiers in Cellular Bioenergetics (Papa, S., Guerrieri, F. and Tager, J.M., eds.), Kluwer Academic/Plenum Publishers, New York, 1999:489-519.
7. Stipani, V., Cappello, A.R., Daddabbo, L., Natuzzi, D., Miniero, D.V., Stipani I., and Palmieri, F. The mitochondrial oxoglutarate carrier: cysteine scanning mutagenesis of transmembrane domain IV and sensitivity of cys mutants to sulphydryl reagents. Biochemistry 2001;40:15805-15810.
8. Cappello, A.R., Curcio, R., Miniero, D. V., Stipani, I., Bisaccia et al. Robinson, A.J., Kunji, E.R., Palmieri F. Functional and structural role of amino acids residues in the even-numbered transmembrane α-helices of the bovine mitochondrial oxoglutarate carrier. J. Mol. Biol. 2006;363:51-62.
9. Cappello, A.R., Miniero, D.V., Curcio, R., Ludovico, A., Daddabbo, L, Stipani, I., Robinson, A.J., Kunji, E.R., Palmieri F. Functional and structural role of amino acids residues in the odd-numbered transmembrane a-helices of the bovine mitochondrial oxoglutarate carrier. J. Mol. Biol. 2007;369:400-412.
10. Castiglione-Morelli, M.A., Ostuni, A., Pepe, A., Lauria, G., Palmieri, F., Bisaccia, F. Solution structure of the first and second transmembrane segments of the mitochondrial oxoglutarate carrier. Mol. Membr. Biol. 2004;21:297-305.
11. Castiglione-Morelli, M.A., Ostuni, A., Croce, F., Palmieri, F., Bisaccia, F. Solution structure of the fifth and sixth transmembrane segments of the mitochondrial oxoglutarate carrier. Mol. Membr. Biol. 2005;22:191-201.
12. Piotto, M., Saudek, V., Sklenar, V. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J. Biomol. NMR 1992;2:661-665.
13. Piantini, U., Sorensen, O. W., Ernst, Richard, R. Multiple quantum filters for elucidating NMR coupling networks. J. Am. Chem. Soc. 1982; 104:6800-6801.
14. Bax, A. and Davis, D.G. MLEV-17-based two dimensional homonuclear magnetization transfer spectroscopy. J. Magn. Reson. 1985;65:355-366.
15. Jeener, J., Meier, B.H., Bachmann, P., Ernst, R.R. Investigation of exchange processes by two dimensional NMR spectroscopy. J. Chem. Phys. 1979;71:4546-4553.
16. Wüthrich, K. NMR of proteins and nucleic acids (1986) J. Wiley, New York.
17. Wüthrich, K., Billeter, M., Braun, W. Pseudo structures for the 20 common amino acids for use in studies of protein conformations by measurements of intramolecular proton-proton distance constraints with nuclear magnetic resonance. J. Mol. Biol. 1983;169:949-961.
18. Guntert, P., Mumenthaler, M., Wüthrich, K. Torsion angle dynamics for NMR structure calculation with the new program DYANA. J. Mol. Biol. 1997;273:283-298.
19. Koradi, R., Billeter, M., Wuthrich, K. MOLMOL: a program for display and analysis of macromolecular structures. J. Mol. Graph. 1996;14:51-55.
20. Gasteiger, E., Gattiker, A., Hoogland, C, Ivanyi, I., Appel, R.D., Bairoch, A. ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res. 2003;31:3784-3788.
21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., Higgins, D.G. The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;24:4876-4882.
22. Palmieri, F., Agrimi, G., Blanco, E., Castegna, A., Di Noia, M.A., lacobazzi, V., Lasorsa, F.M., Marobbio, C.M.T., Palmieri, L, Scarcia, P., Todisco, S., Vozza, A., Walker, J. Identification of mitochondrial carriers in Saccharomyces cerevisiae by transport assay of reconstituted recombinant proteins. Biochem. Biophys. Acta 2006;1757:1249-1262.
23. Robinson, A.J., Kunji, E.R. Mitochondrial carriers in the cytoplasmic state have a common substrate binding site. Proc. Natl. Acad. Sci. USA 2006; 103, 2617-2622.
24. Raussens, V., Slupsky, CM., Ryan, R.O., Sykes, B.D. NMR structure and dynamics of a receptor active apolipoprotein E peptide. J Biol Chem 2002; 277:29172-29180.
25. Buck, M. Trifluoroethanol and colleagues:cosolvents come of age. Recent studies with peptides and proteins. Q. Rev. Biophys. 1998;31:297-355.
26. Chopra, A., Yeagle, P.L., Alderfer, J.L., Albert, A.D. Solution structure of the sixth transmembrane helix of the G-protein-coupled receptor, rhodopsin. Biochem. Biophys. Acta 2000;1463:1-5.
27. Katragadda, M., Alderfer, J.L., Yeagle, P.L. Assembly of a polytopic membrane protein structure from the solution structures of overlapping peptide fragments of bacteriorhodopsin. Biophys. J. 2001;81:1029-1036.