Development of protocols for detection of Colletotrichum acutatum and monitoring of strawberry anthracnose using real-time PCR

Plant Pathology

Volumn 58, Issue 1, 2009, Pages 43-51

  Garrido, C ^a   Carbu M ^a   Fernandez Acero F J ^a   Boonham, N ^b   Colyer, A ^b   Cantoral, J M ^a   Budge, G ^b  

^a UNIVERSITY OF CÁDIZ   (Spain)

^b CENTRAL SCIENCE LABORATORY   (United Kingdom)

Author keywords

Colletotrichum; Diagnosis; ELISA; Fragaria x ananassa; PCR; Strawberry; TaqMan

Indexed keywords

CULTIVAR; DETECTION METHOD; ENVIRONMENTAL MONITORING; FUNGAL DISEASE; FUNGUS; HERB; IMMUNOASSAY; POLYMERASE CHAIN REACTION; REAL TIME;

COLLETOTRICHUM; FRAGARIA X ANANASSA; GLOMERELLA ACUTATA;

EID: 58249103987     PISSN: 00320862     EISSN: 13653059     Source Type: Journal    
DOI: 10.1111/j.1365-3059.2008.01933.x     Document Type: Article

References (30)

1. Abang MM, Winter S, Green KR, Hoffmann P, Mignouna HD, Wolf GA, 2002. Molecular identification of Colletotrichum gloeosporioides causing yam anthracnose in Nigeria. Plant Pathology 51, 63 71.
2. Ansari KI, Palacios N, Araya C, Langin T, Egan D, Doohan FM, 2004. Pathogenic and genetic variability among Colletotrichum lindemuthianum isolates of different geographic origins. Plant Pathology 53, 635 42.
3. Bonde MR, Peterson GL, Maas JL, 1991. Isozyme comparisons for identification of Colletotrichum species pathogenic to strawberry. Phytopathology 81, 1523 8.
4. Boonham N, Pérez LG, Mendez MS, et al., 2004. Development of a real-time RT-PCR assay for the detection of potato spindle tuber viroid. Journal of Virological Methods 116, 139 46.
5. Cullen DW, Lees AK, Toth IK, Duncan JM, 2002. Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR. Plant Pathology 51, 281 92.
6. Denoyes-Rothan B, Baudry A, 1995. Species identification and pathogenicity study of French Colletotrichum strains isolated from strawberry using morphological and cultural characteristics. Phytopathology 85, 53 7.
7. Denoyes-Rothan B, Guérin G, Délye C, et al., 2003. Genetic diversity and pathogenic variability among isolates of Colletotrichum species from strawberry. Phytopathology 93, 219 28.
8. EPPO, 2004. Diagnostic protocols for regulated pests. Glomerella acutata. EPPO Bulletin 34, 193 9.
9. Freeman S, Katan T, 1997. Identification of Colletotrichum species responsible for anthracnose and root necrosis of strawberry in Israel. Phytopathology 87, 516 21.
10. Freeman S, Minz D, Jurkevitch E, Maymon M, Shabi E, 2000. Molecular analyses of Colletotrichum species from almond and other fruit. Phytopathology 90, 608 14.
11. Garrido C, Carbú M, Fernández-Acero FJ, et al., 2008. Isolation and pathogenicity of Colletotrichum spp. causing anthracnose of strawberry in south west Spain. European Journal of Plant Pathology 120, 409 15.
12. Gil-Salas FM, Morris J, Colyer A, et al., 2007. Development of real-time RT-PCR assays for the detection of Cucumber vein yellowing virus (CVYV) and Cucurbit yellow stuning disorder virus (CYSDV) in the whitefly vector Bemisia tabaci. Journal of Virological Methods 146, 45 51.
13. Hughes KJD, Lane CR, Cook RTA, 1997. Development of a rapid method for the detection and identification of Colletotrichum acutatum. In : Dehne HW, Adam G, Diekmann M, Frahm J, Mauler-Machnik A, van Halteren P, eds. Diagnosis and Identification of Plant Pathogens. Dordrecht, theNetherlands : Kluwer, 113 6.
14. Hughes KJD, Tomlinson JA, Griffin RL, Boonham N, Inman AJ, Lane CR, 2006. Development of a one-step real-time polymerase chain reaction assay for diagnosis of Phytophthora ramorum. Phytopathology 96, 975 81.
15. Martínez-Culebras PV, Querol A, Suárez-Fernández MB, García-López MD, Barrio E, 2003. Phylogenetic relationships among Colletotrichum pathogens of strawberry and design of PCR primers for their identification. Journal of Phytopathology 151, 135 43.
16. Mertely JC, Legard DE, 2004. Detection, isolation, and pathogenicity of Colletotrichum spp. from strawberry petioles. Plant Disease 88, 407 12.
17. Mills PR, Sreenivasaprasad S, Brown AE, 1992. Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR. FEMS Microbiology Letters 98, 137 44.
18. Mumford R, Skelton A, Metcalfe E, Walsh K, Boonham N, 2004. The reliable detection of barley yellow and mild mosaic viruses using real-time PCR (TaqMan(R)). Journal of Virological Methods 117, 153 9.
19. Peres NAR, Kuramae EE, Dias MSC, De Souza NL, 2002. Identification and characterization of Colletotrichum spp. affecting fruit after harvest in Brazil. Journal of Phytopathology 150, 128 34.
20. Rodríguez-Guerra R, Ramírez-Rueda MT, de la Vega OM, Simpson J, 2003. Variation in genotype, pathotype and anastomosis groups of Colletotrichum lindemuthianum isolates from Mexico. Plant Pathology 52, 228 35.
21. Simmonds JH, 1965. A study of the species of Colletotrichum causing ripe fruit rots in Queensland. Queensland Journal of Agriculture and Animal Science 22, 437 45.
22. Smith IM, McNamara DG, Scott PR, Holderness M, 1997. Quarantine Pests for Europe. 2nd edn. Wallingford, UK : CABI International.
23. Sreenivasaprasad S, Brown AE, Mills PR, 1992. DNA sequence variation and interrelationships among Colletotrichum species causing strawberry anthracnose. Physiological and Molecular Plant Pathology 41, 265 81.
24. Sreenivasaprasad S, Sharada K, Brown AE, Mills PR, 1996. PCR-based detection of Colletotrichum acutatum on strawberry. Plant Pathology 45, 650 5.
25. Suárez-Fernández MB, Walsh K, Boonham N, O'Neill T, Pearson S, Barker I, 2005. Development of real-time PCR (TaqMan®) assays for the detection and quantification of Botrytis cinerea in planta. Plant Physiology and Biochemistry 43, 890 9.
26. Theodoro GF, Peres NAR, Verona LAF, 2004. Outbreak of postbloom fruit drop of citrus, caused by Colletotrichum acutatum, in Santa Catarina State, southern Brazil. Plant Pathology 53, 253.
27. Weller SA, Elphinstone JG, Smith NC, Boonham N, Stead DE, 2000. Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan®) assay. Applied and Environmental Microbiology 66, 2853 8.
28. White TJ, Bruns SL, Taylor JW, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In : Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: A Guide to Methods and Applications. New York, USA : Academic Press, Inc., 315 22.
29. Whitelaw-Weckert MA, Curtin SJ, Huang R, Steel CC, Blanchard CL, Roffey PE, 2007. Phylogenetic relationships and pathogenicity of Colletotrichum acutatum isolates from grape in subtropical Australia. Plant Pathology 56, 448 63.
30. Yoshida S, Tsukiboshi T, Shinohara H, Koitabashi M, Tsushima S, 2007. Occurrence and development of Colletotrichum acutatum on symptomless blueberry bushes. Plant Pathology 56, 871 7.