Large-scale preparation of active caspase-3 in E. coli by designing its thrombin-activatable precursors

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Kang, Hyo Jin ^a,b   Lee, Young Mi ^b   Jeong, Yu Jin ^a,b   Park, Kyoungsook ^b   Jang, Mi ^b   Park, Sung Goo ^b   Bae, Kwang Hee ^b   Kim, Moonil ^a,b   Chung, Sang J ^a,b  

^a University of Science and Technology (UST)   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINES; AMINO ACIDS; CHROMATOGRAPHIC ANALYSIS; MUTAGENS; ORGANIC ACIDS; PROTEINS; PURIFICATION;

AMINO ACID SEQUENCES; CASPASE-3; CELLULAR SUBSTRATES; DRUG DISCOVERY RESEARCHES; E. COLI; ENGINEERED SITES; ENHANCED CATALYTIC ACTIVITIES; HIGH YIELDS; KINETIC EVALUATIONS; NEURODEGENERATIVE DISEASES; NOVEL METHODS; ORDERS OF MAGNITUDES; PRECURSOR PROTEINS; SOLUBLE PROTEINS;

ESCHERICHIA COLI;

CASPASE 3; PROTEIN PRECURSOR; RECOMBINANT ENZYME; THROMBIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BACTERIUM CULTURE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVATION; ESCHERICHIA COLI; HYDROLYSIS; NONHUMAN; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; BIOSYNTHESIS; GENE EXPRESSION; GENETICS; HUMAN; METABOLISM; TUMOR CELL LINE;

CASPASE 3; CELL LINE, TUMOR; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; PROTEIN ENGINEERING; PROTEIN PRECURSORS; THROMBIN;

EID: 58249100078     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-92     Document Type: Article

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