Comparison of the In vitro Safety of Intraocular Dyes Using Two Retinal Cell Lines: A Focus on Brilliant Blue G and Indocyanine Green

American Journal of Ophthalmology

Volumn 147, Issue 2, 2009, Pages

  Yuen, Darana ^a   Gonder, John ^a   Proulx, Alain ^a   Liu, Hong ^a   Hutnik, Cindy ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BRILLIANT BLUE G; DYE; EVANS BLUE; INDOCYANINE GREEN; TRYPAN BLUE; UNCLASSIFIED DRUG;

ARTICLE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ASSAY; EXPOSURE VARIABLE; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MUELLER CELL; OSMOLARITY; PIGMENT EPITHELIUM; PRIORITY JOURNAL; RETINA GANGLION CELL; RISK ASSESSMENT;

EID: 58249096060     PISSN: 00029394     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ajo.2008.08.031     Document Type: Article

References (78)

1. Fine B.S. Limiting membranes of the sensory retina and pigment epithelium: an electron microscopic study. Arch Ophthalmol 66 (1961) 847-860
2. Sach J., Karel I., Kalvodová B., and Dotrelová D. Ultra-structural analysis of tissue removed during surgery of idiopathic macular holes. Cesk Slov Oftalmol 56 (2000) 286-292
3. Clarkson J.G., Green W.R., and Massof D. A histopathologic review of 168 cases of preretinal membrane. Am J Ophthalmol 84 (1977) 1-17
4. Michels R.G. A clinical and histopathologic study of epiretinal membranes affecting the macula and removed by vitreous surgery. Trans Am Ophthalmol Soc 80 (1982) 580-656
5. Smiddy W.E., Green W.R., Michels R.G., and de la Cruz Z. Ultrastructural studies of vitreomacular traction syndrome. Am J Ophthalmol 107 (1989) 177-185
6. Smiddy W.E., Michels R.G., de Bustros S., et al. Histopathology of tissue removed during vitrectomy for impending macular holes. Am J Ophthalmol 108 (1989) 360-364
7. Guyer D.R., Green W.R., de Bustros S., and Fine S.L. Histopathologic features of idiopathic macular holes and cysts. Ophthalmology 97 (1990) 1045-1051
8. Smiddy W.E., Michels R.G., and Green W.R. Morphology, pathology, and surgery of idiopathic vitreoretinal disorders. A review. Retina 10 (1990) 288-296
9. Yooh H.S., Brooks Jr. H.L., Capone Jr. A., L'Hernault N.L., and Grossniklaus H.E. Ultra-structural features of tissue removed during idiopathic macular hole surgery. Am J Ophthalmol 122 (1996) 67-75
10. Mester V., and Kuhn F. Internal limiting membrane removal in the management of macular holes. Am J Ophthalmol 129 (2000) 769-777
11. Park D.W., Sipperley J.O., Sneed S.R., Dugel P.U., and Jacobsen J. Macular hole surgery with internal limiting membrane peeling and intravitreous air. Ophthalmology 106 (1999) 1392-1398
12. Eckardt C., Eckardt U., Groos S., Luciano L., and Reale E. Removal of the internal limiting membrane in macular holes. Ophthalmologe 94 (1997) 545-551
13. Brooks Jr. H.L. Macular hole surgery with and without internal limiting membrane peeling. Ophthalmology 107 (2000) 1939-1948
14. Al-Abdulla N.A., Thompson J.T., and Sjaarda R.N. Results of macular hole surgery with and without epiretinal dissection or internal limiting membrane removal. Ophthalmology 111 (2004) 142-149
15. Hillenkamp J., Saikia P., Gora F., et al. Macular function and morphology after peeling of idiopathic epiretinal membrane with and without the assistance of indocyanine green. Br J Ophthalmol 89 (2005) 437-443
16. Kwok A.K., Lai T.Y., Yuen K.S., Tam B.S., and Wong V.W. Macular hole surgery with or without indocyanine green stained internal limiting membrane peeling. Clin Experiment Ophthalmol 31 (2003) 470-475
17. Sheidow T.G., Blinder K.J., Holekamp N., et al. Outcome results in macular hole surgery: an evaluation of internal limiting membrane peeling with and without indocyanine green. Ophthalmology 110 (2003) 1697-1701
18. Gandorfer A., Messmer E.M., Ulbig M.W., and Kampik A. Indocyanine green selectively stains the internal limiting membrane. Am J Ophthalmol 131 (2001) 387-388
19. Burk S.E., Da Mata A.P., Snyder M.E., et al. Indocyanine green assisted peeling of the retinal internal limiting membrane. Ophthalmology 107 (2000) 2010-2014
20. Sorcinelli R. Surgical management of epiretinal membrane with indocyanine-green-assisted peeling. Ophthalmologica 217 (2003) 107-110
21. Kwok A.K., Li W.W., Pang C.P., et al. Indocyanine green staining and removal of internal limiting membrane in macular hole surgery: histology and outcome. Am J Ophthalmol 132 (2001) 178-183
22. Kadonosono K., Itoh N., Uchio E., et al. Staining of internal limiting membrane in macular hole surgery. Arch Ophthalmol 118 (2000) 1116-1118
23. Kwok A.K., Lai T.Y., Man-Chan W., and Woo D.C. Indocyanine green-assisted retinal internal limiting membrane removal in stage 3 or 4 macular hole surgery. Br J Ophthalmol 87 (2003) 71-74
24. Da Mata A.P., Burk S.E., Riemann C.D., et al. Indocyanine-green-assisted peeling of the retinal internal limiting membrane during vitrectomy surgery for macular hole repair. Ophthalmology 108 (2001) 1187-1192
25. Da Mata A.P., Burk S.E., Foster R.E., et al. Long-term follow-up of indocyanine green-assisted peeling of the retinal internal limiting membrane during vitrectomy surgery for idiopathic macular hole repair. Ophthalmology 111 (2004) 2246-2253
26. Weinberger A.W., Schlossmacher B., Dahlke C., Hermel M., Kirchhof B., and Schrage N.F. Indocyanine-green-assisted internal limiting membrane peeling in macular hole surgery-a follow-up study. Graefes Arch Clin Exp Ophthalmol 240 (2002) 913-917
27. Wolf S., Reichel M.B., Wiedemann P., and Schnurrbusch U.E. Clinical findings in macular hole surgery with indocyanine green-assisted peeling of the internal limiting membrane. Graefes Arch Clin Exp Ophthalmol 241 (2003) 589-592
28. Kwok A.K., Lai T.Y., Li W.W., Yew D.T., and Wong V.W. Trypan blue- and indocyanine green-assisted epiretinal membrane surgery: clinical and histopathological studies. Eye 18 (2004) 882-888
29. Hillenkamp J., Saikia P., Gora F., et al. Macular function and morphology after peeling of idiopathic epiretinal membrane with and without the assistance of indocyanine green. Br J Ophthalmol 89 (2005) 437-443
30. Lochhead J., Jones E., Chui D., et al. Outcome of ICG-assisted ILM peel in macular hole surgery. Eye 18 (2004) 804-808
31. Enaida H., Sakamoto T., Hisatomi T., et al. Morphological and functional damage of the retina caused by intravitreous indocyanine green in rat eyes. Graefes Arch Clin Exp Ophthalmol 240 (2002) 209-213
32. Gandorfer A., Haritoglou C., Gass C.A., et al. Indocyanine green-assisted peeling of the internal limiting membrane may cause retinal damage. Am J Ophthalmol 132 (2001) 431-433
33. Ikagawa H., Yoneda M., Iwaki M., et al. Chemical toxicity of indocyanine green damages retinal pigment epithelium. Invest Ophthalmol Vis Sci 46 (2005) 2531-2539
34. Rezai K.A., Farrokh-Siar L., Ernest J.T., et al. Indocyanine green induces apoptosis in human retinal pigment epithelial cells. Am J Ophthalmol 137 (2004) 931-933
35. Czajka M.P., McCuen B.W., Cummings T.J., et al. Effects of indocyanine green on the retina and retinal pigment epithelium in a porcine model of retinal hole. Retina 24 (2004) 275-282
36. Sato Y., Tomita H., Sugano E., Isago H., Yoshida M., and Tamai M. Evaluation of indocyanine green toxicity to rat retinas. Ophthalmologica 220 (2006) 153-158
37. Iriyama A., Uchida S., Yanagi Y., et al. Effects of indocyanine green on retinal ganglion cells. Invest Ophthalmol Vis Sci 45 (2004) 943-947
38. Murata M., Shimizu S., Horiuchi S., et al. The effect of indocyanine green on cultured retinal glial cells. Retina 25 (2005) 75-80
39. Ho J.D., Tsai R.J., Chen S.N., and Chen H.C. Cytotoxicity of indocyanine green on retinal pigment epithelium: implications for macular hole surgery. Arch Ophthalmol 121 (2003) 1423-1429
40. Engelbrecht N.E., Freeman J., Sternberg Jr. P., et al. Retinal pigment epithelial changes after macular hole surgery with indocyanine green-assisted internal limiting membrane peeling. Am J Ophthalmol 133 (2002) 89-94
41. Gass C.A., Haritoglou C., Schaumberger M., and Kampik A. Functional outcome of macular hole surgery with and without indocyanine green-assisted peeling of the internal limiting membrane. Graefes Arch Clin Exp Ophthalmol 241 (2003) 716-720
42. Gandorfer A., Haritoglou C., Gandorfer A., et al. Retinal damage from ICG in experimental macular surgery. Invest Ophthalmol Vis Sci 44 (2003) 316-323
43. Haritoglou C., Gandorfer A., Gass C.A., et al. Indocyanine green-assisted peeling of the internal limiting membrane in macular hole surgery affects visual outcome: a clinicopathologic correlation. Am J Ophthalmol 134 (2002) 836-841
44. Ando F., Sasano K., Ohba N., et al. Anatomic and visual outcomes after indocyanine green-assisted peeling of the retinal internal limiting membrane in idiopathic macular hole surgery. Am J Ophthalmol 137 (2004) 609-614
45. Haritoglou C., Gandorfer A., Gass C.A., et al. The effect of indocyanine green on functional outcome of macular pucker surgery. Am J Ophthalmol 135 (2003) 328-337
46. Tsuiki E., Fujikawa A., Miyamura N., Yamada K., Mishima K., and Kitaoka T. Visual field defects after macular hole surgery with indocyanine green-assisted internal limiting membrane peeling. Am J Ophthalmol 143 (2007) 704-705
47. Uemura A., Kanda S., Sakamoto Y., and Kita H. Visual field defects after uneventful vitrectomy for epiretinal membrane with indocyanine green-assisted internal limiting membrane peeling. Am J Ophthalmol 136 (2003) 252-257
48. Weinberger A.W., Kirchhof B., Mazinani B.E., and Schrage N.F. Persistent indocyanine green (ICG) fluorescence 6 weeks after intraocular ICG administration for macular hole surgery. Graefes Arch Clin Exp Ophthalmol 239 (2001) 388-390
49. Ashikari M., Ozeki H., Tomida K., Sakurai E., Tamai K., and Ogura Y. Retention of dye after indocyanine green-assisted internal limiting membrane peeling. Am J Ophthalmol 136 (2003) 172-174
50. Enaida H., Hisatomi T., Goto Y., et al. Preclinical investigation of internal limiting membrane staining and peeling using intravitreal brilliant blue G. Retina 26 (2006) 623-630
51. Enaida H., Hisatomi T., Hata Y., et al. Brilliant blue G selectively stains the internal limiting membrane/brilliant blue G-assisted membrane peeling. Retina 26 (2006) 631-636
52. Congdon R.W., Muth G.W., and Splittgerber A.G. The binding interaction of Coomassie blue with proteins. Anal Biochem 213 (1993) 407-413
53. Laughton C. Quantification of attached cells in microtiter plates based on Coomassie brilliant blue G-250 staining of total cellular protein. Anal Biochem 140 (1984) 417-423
54. Westermeier R. Sensitive, quantitative, and fast modifications for Coomassie blue staining of polyacrylamide gels. Proteomics 6 (2006) 61-64
55. Said-Fernández S., González-Garza M.T., Mata-Cárdenas B.D., and Navarro-Marmolejo L. A multi-purpose solid-phase method for protein determination with Coomassie brilliant blue G-250. Anal Biochem 191 (1990) 119-126
56. Burgess-Cassler A., Johansen J.J., Santek D.A., Ide J.R., and Kendrick N.C. Computerized quantitative analysis of Coomassie-blue-stained serum proteins separated by two-dimensional electrophoresis. Clin Chem 35 (1989) 2297-2304
57. Saoji A.M., Kulkarni M., and Naghi T. Coomassie brilliant blue G-250 as a highly sensitive pre-stain for immediate visualization of human serum proteins on polyacrylamide gel disc electrophoresis and raising monospecific antibodies. Indian J Pathol Microbiol 32 (1989) 286-290
58. Oshima I., Kohama T., and Kodaira T. A sensitive assay for serum acid soluble proteins with Coomassie brilliant blue G-250 and its application for autoimmune diseases. Tokushima J Exp Med 34 (1987) 23-27
59. Bergqvist Y., Karlsson L., and Fohlin L. Total protein determined in human breast milk by use of Coomassie brilliant blue and centrifugal analysis. Clin Chem 35 (1989) 2127-2129
60. Hisatomi T., Enaida H., Matsumoto H., et al. Staining ability and biocompatibility of brilliant blue G: preclinical study of brilliant blue G as an adjunct for capsular staining. Arch Ophthalmol 124 (2006) 514-519
61. Baptiste D.C., Hartwick A.T., Jollimore C.A., et al. Comparison of the neuroprotective effects of adrenoceptor drugs in retinal cell culture and intact retina. Invest Ophthalmol Vis Sci 43 (2002) 2666-2676
62. Perrier M., and Sebag M. Trypan blue-assisted peeling of the internal limiting membrane during macular hole surgery. Am J Ophthalmol 135 (2003) 903-905
63. Haritoglou C., Eibl K., Schaumberger M., et al. Functional outcome after Trypan blue-assisted vitrectomy for macular pucker: a prospective, randomized, comparative trial. Am J Ophthalmol 138 (2004) 1-5
64. Narayanan R., Kenney M.C., Kamjoo S., et al. Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells. Invest Ophthalmol Vis Sci 46 (2005) 304-309
65. Haritoglou C., Gandorfer A., Schaumberger M., et al. Trypan blue in macular pucker surgery: an evaluation of histology and functional outcome. Retina 24 (2004) 582-590
66. Veckeneer M., van Overdam K., Monzer J., et al. Ocular toxicity study of Trypan blue injected into the vitreous cavity of rabbit eyes. Graefes Arch Clin Exp Ophthalmol 239 (2001) 698-704
67. Rezai K.A., Farrokh-Siar L., Gasyna E.M., and Ernest J.T. Trypan blue induces apoptosis in human retinal pigment epithelial cells. Am J Ophthalmol 138 (2004) 492-495
68. Jin Y., Uchida S., Yanagi Y., Aihara M., and Araie M. Neurotoxic effects of Trypan blue on rat retinal ganglion cells. Exp Eye Res 81 (2005) 395-400
69. Gale J.S., Proulx A.A., Gonder J.R., Mao A.J., and Hutnik C.M. Comparison of the in vitro toxicity of indocyanine green to that of Trypan blue in human retinal pigment epithelium cell cultures. Am J Ophthalmol 138 (2004) 64-69
70. Jackson T.L., Hillenkamp J., Knight B.C., et al. Safety testing of indocyanine green and Trypan blue using retinal pigment epithelium and glial cell cultures. Invest Ophthalmol Vis Sci 45 (2004) 2778-2785
71. Kodjikian L., Richter T., Halberstadt M., et al. Toxic effects of indocyanine green, infracyanine green, and Trypan blue on the human retinal pigmented epithelium. Graefes Arch Clin Exp Ophthalmol 243 (2005) 917-925
72. Lee K.L., Dean S., and Guest S. A comparison of outcomes after indocyanine green and Trypan blue-assisted internal limiting membrane peeling during macular hole surgery. Br J Ophthalmol 89 (2005) 420-424
73. Li K., Wong D., Hiscott P., Stanga P., Groenewald C., and McGalliard J. Trypan blue staining of internal limiting membrane and epiretinal membrane during vitrectomy: visual results and histopathological findings. Br J Ophthalmol 87 (2003) 216-219
74. Rodrigues E.B., Meyer C.H., Schmidt J.C., and Kroll P. Trypan blue stains the epiretinal membrane but not the internal limiting membrane. Br J Ophthalmol 87 (2003) 1431-1432
75. Horobin R.W., and Kiernan J.A. Conn's biological stains: a handbook of dyes, stains and fluorochromes for use in biology and medicine. Tenth Edition (2002), BIOS Scientific Publishers Ltd, Oxford
76. Hawkins B.T., and Egleton R.D. Fluorescence imaging of blood-brain barrier disruption. J Neurosci Methods 151 (2006) 262-267
77. Smith C.E., Atchabahian A., Mackinnon S.E., and Hunter D.A. Development of the blood-nerve barrier in neonatal rats. Microsurgery 21 (2001) 290-297
78. Patterson C.E., Rhoades R.A., and Garcia J.G. Evans blue dye as a marker of albumin clearance in cultured endothelial monolayer and isolated lung. J Appl Physiol 72 (1992) 865-873