Rapid, semi-automated, and inexpensive radioimmunoassay of cAMP: Application in GPCR-mediated adenylate cyclase assays

Journal of Neuroscience Methods

Volumn 177, Issue 2, 2009, Pages 261-266

  Brown, Justin T ^a   Kant, Andrew ^a   Mailman, Richard B ^a,b  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b PENN STATE COLLEGE OF MEDICINE   (United States)

Author keywords

3 ,5 Adenosine cyclic monophosphate; Adenylate cyclase; Adenylyl cyclase; cAMP; cyclic AMP; Cyclic nucleotides; G protein coupled receptors; Radioimmunoassays; Receptor signaling

Indexed keywords

ADENYLATE CYCLASE; CYCLIC AMP; DOPAMINE 1 RECEPTOR; ENZYME ANTIBODY; GUANINE NUCLEOTIDE BINDING PROTEIN; IODINE 125; MOLECULAR MARKER;

ACCURACY; ARTICLE; AUTOANALYSIS; COST CONTROL; FILTRATION; HUMAN; HUMAN CELL; MAGNETISM; PRIORITY JOURNAL; RADIOIMMUNOASSAY; SENSITIVITY AND SPECIFICITY; SEPARATION TECHNIQUE; TIME;

ADENYLATE CYCLASE; ANTIBODIES; AUTOMATION; CELL LINE; COSTS AND COST ANALYSIS; CYCLIC AMP; CYCLIC GMP; HUMANS; IODINE RADIOISOTOPES; LABORATORY TECHNIQUES AND PROCEDURES; MAGNETICS; MICROSPHERES; RADIOIMMUNOASSAY; RECEPTORS, G-PROTEIN-COUPLED; REPRODUCIBILITY OF RESULTS; SECOND MESSENGER SYSTEMS; TIME FACTORS;

EID: 58149521948     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2008.10.016     Document Type: Article

References (18)

1. Antoni F.A. Molecular diversity of cyclic AMP signalling. Front Neuroendocrinol 21 (2000) 103-132
2. Brewster W.K., Nichols D.E., Riggs R.M., Mottola D.M., Lovenberg T.W., Lewis M.H., et al. trans-10,11-dihydroxy-5,6,6a,7,8,12b-hexahydrobenzo[a]phenanthridine: a highly potent selective dopamine D1 full agonist. J Med Chem 33 (1990) 1756-1764
3. Brooker G., Terasaki W.L., and Price M.G. Gammaflow: a completely automated radioimmunoassay system. Science 194 (1976) 270-276
4. Brown B.L., Ekins R.P., and Albano J.D. Saturation assay for cyclic AMP using endogenous binding protein. Adv Cyclic Nucleotide Res 2 (1972) 25-40
5. Ekins R.P., and Brown B.L. Cyclic nucleotides and thyroid hormones: radioimmunoassay or protein-binding assay?. Biochem J 126 (1972) 1P
6. Hanoune J., and Defer N. Regulation and role of adenylyl cyclase isoforms. Annu Rev Pharmacol Toxicol 41 (2001) 145-174
7. Harper J.F., and Brooker G. Femtomole sensitive radioimmunoassay for cyclic AMP and cyclic GMP after 2'0 acetylation by acetic anhydride in aqueous solution. J Cyclic Nucleotide Res 1 (1975) 207-218
8. Mailman RB, Boyer J. Theory and practice of receptor characterization and drug analysis; 1997. http://www.pdg.cnb.uam.es/cursos/Barcelona2002/pages/Farmac/Comput_Lab/Radioligandos/Mailman_Boyer/index.htm.
9. McPhee I., Gibson L.C., Kewney J., Darroch C., Stevens P.A., Spinks D., et al. Cyclic nucleotide signalling: a molecular approach to drug discovery for Alzheimer's disease. Biochem Soc Trans 33 (2005) 1330-1332
10. Motulsky HJ. Error in counting radioactivity; 2007. http://www.graphpad.com/articles/AZIntroStats_files/frame.htm#slide0271.htm.
11. Oehlenschlager W.F., Kubalak S.W., and Currie M.G. A rapid and economical method of preparing radioiodinated cyclic nucleotide derivatives for use in radioimmunoassays. J Immunoassay 11 (1990) 109-118
12. Post S.R., Ostrom R.S., and Insel P.A. Biochemical methods for detection and measurement of cyclic AMP and adenylyl cyclase activity. Methods Mol Biol 126 (2000) 363-374
13. Salomon Y. Adenylate cyclase assay. Adv Cyclic Nucleotide Res 10 (1979) 35-55
14. Schulz R., and Blum V. RIA determination and immunofluorescence localization of cyclic nucleotides in rainbow trout (Salmo gairdneri) testes. Gen Comp Endocrinol 57 (1985) 301-308
15. Steiner A.L., Wehmann R.E., Parker C.W., and Kipnis D.M. Radioimmunoassay for the measurement of cyclic nucleotides. Adv Cyclic Nucleotide Res 2 (1972) 51-61
16. Ullman E.F., Kirakossian H., Singh S., Wu Z.P., Irvin B.R., Pease J.S., et al. Luminescent oxygen channeling immunoassay: measurement of particle binding kinetics by chemiluminescence. Proc Natl Acad Sci USA 91 (1994) 5426-5430
17. Weishaar R.E. Multiple molecular forms of phosphodiesterase: an overview. J Cyclic Nucleotide Protein Phosphor Res 11 (1986) 463-472
18. Williams C. cAMP detection methods in HTS: selecting the best from the rest. Nat Rev Drug Discov 3 (2004) 125-135