Macromolecular crowding in vitro as means of emulating cellular interiors: When less might be more

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 51, 2008, Pages

  Harve, K S ^a   Vigneshwar, R ^a   Rajagopalan, R ^a   Raghunath, M ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHATE; UREA;

CHEMICAL STRUCTURE; IN VITRO STUDY; LETTER; LIGHT SCATTERING; MACROMOLECULE; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR STABILITY; PRIORITY JOURNAL; PROTEIN FOLDING; STRUCTURE ANALYSIS; VELOCITY;

CELLS; DIFFUSION; FICOLL; MACROMOLECULAR SUBSTANCES; MODELS, BIOLOGICAL; POLYMERASE CHAIN REACTION; PROTEIN FOLDING; UREA;

EID: 58149479405     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0810077106     Document Type: Letter

References (4)

1. Homouz D, Perham M, Samiotakis A, Cheung MS, Wittung-Stafshede P (2008) Crowded, cell-like environment induces shape changes in aspherical protein. Proc Natl Acad Sci USA 105:11754-11759.
2. Harve KS, Lareu RR, Rajagopalan R, Raghunath M (2006) Macromolecular crowding in biological systems: Dynamic light scattering (DLS) to quantify the excluded volume effect. Biophys Rev Lett 1:317-325.
3. Lareu RR, Harve KS, Raghunath M (2007) Emulating a crowded intracellular environment in vitro dramatically improves RT-PCR performance. Biochem Biophys Res Commun 363:171-177.
4. Ellis RJ (2001) Macromolecular crowding: Obvious but under-appreciated. Trends Biochem Sci 26:597-604.