Application of screen-printed microband biosensors to end-point measurements of glucose and cell numbers in HepG2 cell culture

Analytical Biochemistry

Volumn 385, Issue 2, 2009, Pages 334-341

  Pemberton, R M ^a   Xu, J ^a   Pittson, R ^b   Biddle, N ^b   Drago, G A ^c   Jackson, S K ^a   Hart, J P ^a  

^a UNIVERSITY OF THE WEST OF ENGLAND   (United Kingdom)

^b Gwent Electronic Materials   (United Kingdom)

^c Applied Enzyme Technology Ltd   (United Kingdom)

Author keywords

Cell culture; Glucose biosensor; Glucose metabolism; HepG2 cells; Microband electrode

Indexed keywords

ACTIVATION ENERGY; BIOASSAY; CELL CULTURE; DYES; ELECTRODES; ENZYME ELECTRODES; GLUCOSE; GLUCOSE SENSORS; INVERSE PROBLEMS; METABOLISM; POINT CONTACTS;

COBALT PHTHALOCYANINE; END-POINT MEASUREMENT; GLUCOSE BIOSENSOR; GLUCOSE CONCENTRATION; GLUCOSE METABOLISM; HEP-G2 CELLS; MICROBAND ELECTRODE; STEADY-STATE CURRENTS;

GLUCOSE OXIDASE;

BUFFER; CARBON; COBALT; GLUCOSE; GLUCOSE OXIDASE; PHTHALOCYANINE; WATER;

ACCURACY; AMPEROMETRY; ARTICLE; BIOSENSOR; CALCULATION; CAUCASIAN; CELL COUNT; CELL CULTURE; CELL STRAIN HEPG2; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; ELECTRODE; ENERGY; GLUCOSE METABOLISM; GLUCOSE TRANSPORT; HEPATOMA CELL; HUMAN; HUMAN CELL; LIVER CELL; MEASUREMENT; MONITORING; PH; PRIORITY JOURNAL; SAMPLE; SPECTROPHOTOMETRY; STEADY STATE; SUPERNATANT;

EID: 58149389911     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.10.037     Document Type: Article

References (18)

1. Hilditch P.I., and Green M.J. Disposable electrochemical biosensors. Analyst 116 (1991) 1217-1220
2. Newman J.D., and Setford S.J. Enzymatic biosensors. Mol. Biotechnol. 32 (2006) 249-268
3. Mathews D.R., Bown E., Watson A., Holman R.R., Steemson J., Hughes S., and Scott D. Pen-sized digital 30-second blood glucose meter. Lancet 1 (1987) 778-779
4. J.P. Hart, A. Crew, E. Crouch, K. Honeychurch, R.M. Pemberton, Recent developments in screen-printed carbon electrode sensors/biosensors for electrochemical analysis, in: S. Alegret, A. Merkoci (Eds.), Comprehensive Analytical Chemistry, vol. 49, Electrochemical Sensor Analysis, Elsevier, Amsterdam, 2007, pp. 495-556.
5. Crouch E., Cowell D.C., Hoskins S., Pittson R.W., and Hart J.P. A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink. Biosens. Bioelectron. 21 (2005) 712-718
6. Crouch E., Cowell D.C., Hoskins S., Pittson R., and Hart J.P. Amperometric, screen-printed, glucose biosensor for analysis of human plasma samples using a biocomposite water-based carbon ink incorporating glucose oxidase. Anal. Biochem. 347 (2005) 17-23
7. Rawson F.J., Purcell W.M., Xu J., Cowell D.C., Fielden P.R., Biddle N., and Hart J.P. Fabrication and characterisation of novel screen-printed tubular microband electrodes and their application to the measurement of hydrogen peroxide. Electrochim. Acta 52 (2007) 7248-7253
8. R.M. Pemberton, R. Pittson, N. Biddle, J.P. Hart, Fabrication of microband glucose biosensors using a screen-printing water-based carbon ink and their application in serum analysis, Biosens. Bioelectron., in press.
9. Frauwirth K.A., Riley J.L., Harris M.H., Parry R.V., Rathmell J.C., Plas D.R., Elstrom R.L., June C.H., and Thompson C.B. The CD28 signalling pathway regulates glucose metabolism. Immunity 16 (2002) 769-777
10. Sidorenko Y., Wahl A., Dauner M., Genzel Y., and Reichl U. Comparison of metabolic flux distributions for MDCK cell growth in glutamine- and pyruvate-containing media. Biotechnol. Prog. 24 (2008) 311-320
11. Wang I.K., Chang K.M., and Ho L. Glucose measurement for cell culture: GlucCell, a modified blood glucose monitor. Genet. Eng. Biotechnol. News 26 10 (2006) 40-43
12. Dunn Labortechnik, GlucCell glucose monitoring system. Available from: .
13. F.J. Rawson, W.M. Purcell, J. Xu, R.M. Pemberton, P.R. Fielden, N. Biddle, J.P. Hart, A microband lactate biosensor fabricated using a water-based screen-printed carbon ink, Talanta, in press.
14. Trinder T. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann. Clin. Biochem. 6 (1969) 24-27
15. Gilmartin M.A.T., Hart J.P., and Patton D.T. Prototype, solid-phase glucose biosensor. Analyst 120 (1995) 1973-1981
16. Hochachka P.W., and Somero G.N. Biochemical Adaptation (1984), Princeton University Press, Princeton, NJ
17. Morris J.G. The kinetics of enzyme-catalysed reactions. In: Barrington E.J.W., and Willis A.J. (Eds). A Biologist's Physical Chemistry (1974), second ed, Edward Arnold, London 312-317
18. 13C-labeling experiments: I. Experimental observations. Biotechnol. Bioeng. 100 (2008) 344-354