Evaluation of real-time RT-PCR compared with conventional RT-PCR for detecting human metapneumovirus RNA from clinical specimens

Japanese Journal of Infectious Diseases

Volumn 61, Issue 6, 2008, Pages 461-464

  Kaida, Atsushi ^a   Kubo, Hideyuki ^a   Shiomi, Masashi ^b   Kohdera, Urara ^c   Iritani, Nobuhiro ^a  

^a OSAKA CITY INSTITUTE OF PUBLIC HEALTH AND ENVIRONMENTAL SCIENCES   (Japan)

^b Osaka Central Hospital   (Japan)

^c Nakano Children's Hospital   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

ADOLESCENT; ADULT; ARTICLE; ASTHMA; BRONCHITIS; CHILD; CONTROLLED STUDY; FEMALE; GENE TARGETING; GENETIC LINKAGE; HUMAN; INFANT; INFLUENZA; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; METAPNEUMOVIRUS; NONHUMAN; NOSE MUCUS; NUCLEOTIDE SEQUENCE; PHYLOGENY; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; SPUTUM ANALYSIS; VIRUS DETECTION; VIRUS ISOLATION; VIRUS PNEUMONIA; VIRUS STRAIN; COMPARATIVE STUDY; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MIDDLE AGED; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; VIROLOGY; VIRUS INFECTION;

ADOLESCENT; CHILD; CHILD, PRESCHOOL; HUMANS; INFANT; METAPNEUMOVIRUS; MIDDLE AGED; PARAMYXOVIRIDAE INFECTIONS; PHYLOGENY; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

EID: 58149386450     PISSN: 13446304     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. van den Hoogen, B.G., de Jong, J.C., Groen, J., et al. (2001): A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat. Med., 7, 719-724.
2. Bastien, N., Ward, D., Van Caeseele, P., et al. (2003): Human metapneumovirus infection in the Canadian population. J. Clin. Microbiol., 41, 4642-4646.
3. Boivin, G., Abed, Y. Pelletier, G., et al. (2002): Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups. I. Infect. Dis., 186, 1330-1334.
4. Peret, T.C., Boivin, G., Li, Y., et al. (2002): Characterization of human metapneumoviruses isolated from patients in North America. J. Infect. Dis., 185, 1660-1663.
5. Boivin, G., Mackay, I., Sloots, T. P., et al. (2004): Global genetic diversity of human metapneumovirus fusion gene. Emerg. Infect. Dis., 10, 1154-1157.
6. van den Hoogen, B.G., Herfst, S., Sprong, L., et al. (2004): Antigenic and genetic variability of human metapneumoviruses. Emerg. Infect. Dis., 10, 658-666.
7. Ebihara, T., Endo, R., Ma, X., et al. (2005): Detection of human metapneumovirus antigens in nasopharyngeal secretions by an immunofluorescent-antibody test. J. Clin. Microbiol., 43, 1138-1141.
8. Landry, M.L., Cohen, S. and Ferguson, D. (2008): Prospective study of human metapneumovirus detection in clinical samples by use of light diagnostics direct immunofluorescence reagent and real-time PCR. J. Clin. Microbiol., 46, 1098-1100.
9. Ieven, M. (2007): Currently used nucleic acid amplification tests for the detection of viruses and atypicals in acute respiratory infections. J. Clin. Virol., 40, 259-276.
10. Cote, S., Abed, Y. and Boivin, G. (2003): Comparative evaluation of real-time PCR assays for detection of the human metapneumovirus. J. Clin. Microbiol., 41, 3631-3635.
11. Maertzdorf, J., Wang, C.K., Brown, J.B., et al. (2004): Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages. J. Clin. Microbiol., 42, 981-986.
12. Deffrasnes, C., Cote, S. and Boivin, G. (2005): Analysis of replication kinetics of the human metapneumovirus in different cell lines by real-time PCR. J. Clin. Microbiol., 43, 488-490.
13. Kuypers, J., Wright, N., Corey, L., et al. (2005): Detection and quantification of human metapneumovirus in pediatric specimens by real-time RT-PCR. J. Clin. Virol., 33, 299-305.
14. Mackay, I.M., Jacob, K.C., Woolhouse, D., et al. (2003): Molecular assays for detection of human metapneumovirus. J. Clin. Microbiol., 41, 100-105.
15. Kaida, A., Iritani, N., Kubo, H., et al. (2006): Seasonal distribution and phylogenetic analysis of human metapneumovirus among children in Osaka City, Japan. J. Clin. Virol., 35, 394-399.
16. Kikuta, H., Sakata, C., Gamo, R., et al. (2008): Comparison of a lateral-flow immunochromatography assay with real-time reverse transcription-PCR for detection of human metapneumovirus. J. Clin. Microbiol., 46, 928-932.