4-dihydrotrisporin-dehydrogenase, an enzyme of the sex hormone pathway of Mucor mucedo: Purification, cloning of the corresponding gene, and developmental expression

Eukaryotic Cell

Volumn 8, Issue 1, 2009, Pages 88-95

  Wetzel, Jana ^a   Scheibner, Olaf ^b,c   Burmester, Anke ^a   Schimek, Christine ^a   Wöstemeyer, Johannes ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b HANS KNÖLL INSTITUTE   (Germany)

^c Thermofisher Scientific   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

4 DIHYDROTRISPORIN DEHYDROGENASE, MUCOR MUCEDO; 4-DIHYDROTRISPORIN DEHYDROGENASE, MUCOR MUCEDO; ALCOHOL DEHYDROGENASE; FUNGAL PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; FUNGAL GENE; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MUCOR; SEQUENCE ALIGNMENT;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; CLONING, MOLECULAR; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, FUNGAL; GENES, MATING TYPE, FUNGAL; MOLECULAR SEQUENCE DATA; MUCOR; NADPH DEHYDROGENASE; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

MUCOR; MUCOR CIRCINELLOIDES; MUCOR MUCEDO; MYXOCOCCUS XANTHUS; PHYCOMYCES BLAKESLEEANUS; PHYCOMYCETES; RHIZOPUS ORYZAE; ZYGOMYCETES;

EID: 58149345211     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00225-08     Document Type: Article

References (38)

1. Arnau, J., and P. Strøman. 1993. Gene replacement and ectopic integration in the zygomycete Mucor circinelloides. Curr. Genet. 23:542-546.
2. Austin, D. J. 1970. The biosynthesis of trisporic acid from β-carotene via retinal and trisporol. Experimentia 26:348-349.
3. Avadhani, M., R. Geyer, D. C. White, and L. J. Shimkets. 2006. Lysophosphatidylethanolamine is a substrate for the short-chain alcohol dehydrogenase SocA from Myxococcus xanthus. J. Bacteriol. 188:8548-8550.
4. Blakeslee, A., and J. Cartledge. 1927. Sexual dimorphism in Mucorales. II. Interspecific reactions. Bot. Gaz. 84:51-57.
5. Bu'Lock, J. D., B. E. Jones, and N. Winskill. 1976. The apocarotenoid system of sex hormones and prohormones in Mucorales. Pure Appl. Chem. 47:191-202.
6. Bu'Lock, J. D., B. E. Jones, S. A. Quarrie, and N. Winskill. 1973. The biochemical basis of sexuality in Mucorales. Naturwissenschaften 60:550-551.
7. Burgeff, H. 1924. Untersuchungen über Sexualität und Parasitismus bei Mucorineen. Bot. Abhandl. 4:5-135.
8. Burmester, A., and K. Czempinski. 1994. Sequence comparison of a segment of the gene for 3-hydroxyl-3-methylglutaryl-coenzyme A reductase in zygomycetes. Eur. J. Biochem. 220:403-408.
9. Burmester, A., M. Richter, K. Schultze, K. Voelz, D. Schachtschabel, W. Boland, J. Wöstemeyer, and C. Schimek. 2007. Cleavage of β-carotene as the first step in sexual hormone synthesis. Fungal Genet. Biol. 44:1096-1108.
10. Czempinski, K., V. Kruft, J. Wöstemeyer, and A. Burmester. 1996. 4-Dihydro-methyltrisporate dehydrogenase from Mucor mucedo, an enzyme of the sexual hormone pathway: purification, and cloning of the corresponding gene. Microbiology 142:2647-2654.
11. Einax, E., and K. Voigt. 2003. Oligonucleotide primers for the universal amplification of β-tubulin genes facilitate phylogenetic analyses in the Regnum fungi. Org. Divers. Evol. 3:185-194.
12. Gessler, N. N., A. V. Sokolov, and T. A. Belozerskaya. 2002. Initial stages of trisporic acid synthesis in Blakeslea trispora. Appl. Biochem. Microbiol. 38:536-543.
13. Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41:95-98.
14. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 136:557-580.
15. Horiuchi, T., M. Taoka, T. Isobe, T. Komano, and S. Inouye. 2002. Role of fruA and csgA genes in gene expression during development of Myxococcus xanthus: analysis by two-dimensional gel electrophoresis. J. Biol. Chem. 277:26753-26760.
16. Idnurm, A., F. J. Walton, A. Floyd, and J. Heitman. 2008. Identification of the sex genes in an early diverged fungus. Nature 451:193-196.
17. Jez, J. M., and T. M. Penning. 2001. The aldo-keto reductase (AKR) superfamily: an update. Chem. Biol. Interact. 130:499-525.
18. Jez, J. M., M. J. Bennett, B. P. Schlegel, M. Lewis, and T. M. Penning. 1997. Comparative anatomy of the aldo-keto-reductase superfamily. Biochem. J. 326:625-636.
19. Kallberg, Y., and B. Persson. 2006. Prediction of coenzyme specificity in dehydrogenases/reductases. A hidden Markov model-based method and its application on complete genomes. FEBS J. 273:1177-1184.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
21. +-containing protein. Genes Dev. 9:2964-2973.
22. Michielse, C. B., K. Salim, P. Ragas, A. F. Ram, B. Kudla, B. Jarry, P. J. Punt, and C. A. van den Hondel. 2004. Development of a system for integrative and stable transformation of the zygomycete Rhizopus oryzae by Agrobacterium-mediated DNA transfer. Mol. Genet. Genomics 271:499-510.
23. Nieuwenhuis, M., and H. van den Ende. 1975. Sex specificity of hormone synthesis in Mucor mucedo. Arch. Microbiol. 102:167-169.
24. Oppermann, U., C. Filling, M. Hult, N. Shafqat, X. Q. Wu, M. Lindh, J. Shafqat, E. Nordling, Y. Kallberg, B. Persson, and H. Jornvall. 2003. Short-chain dehydrogenases/reductases (SDR): the 2002 update. Chem. Biol. Interact. 143:247-253.
25. Rave, N., R. Crkvenjakov, and H. Boedtker. 1979. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Res. 6:3559-3567.
26. Schilde, C., J. Wöstemeyer, and A. Burmester. 2001. Green fluorescent protein as a reporter for gene expression in the mucoralean fungus Absidia glauca. Arch. Microbiol. 175:1-7.
27. Schimek, C., and J. Wöstemeyer. 2005. Pheromone action in the fungal groups Chytridiomycota and Zygomycota, and in the Oomycota, p. 215-231. In U. Kües and R. Fischer (ed.), Mycota I, 2nd ed. Springer, Berlin, Germany.
28. Schimek, C., A. Petzold, K. Schultze, J. Wetzel, F. Wolschendorf, A. Burmester, and J. Wöstemeyer. 2005. 4-Dihydromethyltrisporate dehydrogenase, an enzyme of the sex hormone pathway in Mucor mucedo, is constitutively transcribed but its activity is differently regulated in (+) and (-) mating types. Fungal Genet. Biol. 42:804-812.
29. Schimek, C., K. Kleppe, A. R. Saleem, K. Voigt, A. Burmester, and J. Wöstemeyer. 2003. Sexual reactions in Mortierellales are mediated by the trisporic acid system. Mycol. Res. 107:736-747.
30. Shevchenko, A., M. Wilm, O. Vorm, and M. Mann. 1996. Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels. Anal. Chem. 68:850-858.
31. Simon, A., U. Hellman, C. Wernstedt, and U. Eriksson. 1985. The retinol pigment epithelial specific 11-cis retinol dehydrogenase belongs to the family of short-chain alcohol dehydrogenases. J. Biol. Chem. 20:1107-1112.
32. Smith, P. K., R. I. Krohn, G. T. Hermanson, A. K. Malla, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson, and D. C. Klenk. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76-85.
33. Søgaard-Andersen, L. 2007. Contact-dependent signalling in Myxococcus xanthus: the function of the C-signal in fruiting body morphogenesis, p. 77-92. In D. Whitworth (ed.), Myxobacteria: multicellularity and differentiation. ASM Press, Washington, DC.
34. Sutter, R. P., A. B. Grandin, B. D. Dye, and W. R. Moore. 1996. (-) Mating type-specific mutants of Phycomyces defective in sex pheromone biosynthesis. Fungal Genet. Biol. 20:268-279.
35. Sutter, R. P., and J. P. Whitaker. 1981. Zygophore-stimulating precursors (pheromones) of trisporic acids active in (-)-Phycomyces blakesleeanus: acid-catalyzed anhydro derivatives of methyl-4- dihydrotrisporate C and 4-dihydrotrisporate C. J. Biol. Chem. 256:2334-2341.
36. Werkman, B. A. 1976. Localization and partial characterization of a sex-specific enzyme in homothallic and heterothallic Mucorales. Arch. Microbiol. 109:209-213.
37. Wöstemeyer, J. 1985. Strain-dependent variation in ribosomal DNA arrangement in Absidia glauca. Eur. J. Biochem. 146:443-448.
38. Wöstemeyer, J., and C. Schimek. 2007. Trisporic acid and mating in zygomycetes, p. 431-443. In J. Heitman, J. W. Kronstad, J. W. Taylor, and L. Casselton (ed.), Sex in fungi: molecular determination and evolutionary implications. ASM Press, Washington, DC.