Impact of inactivated extracellular proteases on the modified flagellin type III secretion pathway of bacillus halodurans

Applied and Environmental Microbiology

Volumn 75, Issue 1, 2009, Pages 271-274

  Berger, Eldie ^a   Du Plessis, Erika ^a   Gerber, Isak ^a   Crampton, Michael ^a   Nxumalo, Nolwandle ^a   Louw, Maureen ^a  

^a DSI CSIR Nanotechnology Innovation Centre   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS HALODURANS; EXTRACELLULAR PROTEASES; GENE FUSIONS;

AMINES; BACTERIOLOGY; MONOMERS;

ENZYME ACTIVITY;

BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; PEPTIDE; SECRETION;

BACILLUS; BACTERIAL PROTEINS; FLAGELLIN; GENE DELETION; MEMBRANE TRANSPORT PROTEINS; PEPTIDE HYDROLASES; RECOMBINANT FUSION PROTEINS;

BACILLUS HALODURANS;

EID: 58149333849     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02430-08     Document Type: Article

References (14)

1. Biswas, I., A. Gruss, S. D, Ehrlich, and E. Maguin, 1993. High-efficiency gene inactivation and replacement system for gram-positive bacteria. J. Bac-teriol. 175:3628-3635.
2. Coxon, R. D., C. R. Harwood, and A. R. Archibald. 1991. Protein export during growth of Bacillus subtilis: the effect of extracellular protease deficiency. Lett. Appl. Microbiol. 12:91-94.
3. Crampton, M., E. Berger, S. Reid, and M. E. Louw, 2007. The development of a flagellin surface display expression system in a moderate thermophile. Bacillus halodurans Alk36. Appl. Microbiol. Biotechnol. 75:599-607.
4. Harwood, C, R., R. D, Coxon, and I. C. Hancock. 1990. The Bacillus cell envelope and secretion, p. 327-339. In C. W. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley and Sons, Chichester, United Kingdom.
5. Heussen, C., and E. B. Dowdle. 1980. Electrophoretic analysis of plasmino-gen activators in polyacrylamide gels containing sodium dodecyl sulphate and copolymerized substrates. Anal. Biochem. 102:196-202.
6. Hewer, R., and D. Meyer. 2003. Peptide immunogens based on the envelope region of H1V-1 are recognized by HIV/AIDS patient polyclonal antibodies and induce strong humoral immune responses in mice and rabbits. Mol. Immunol. 40:327-335.
7. Kang, D., Y. S. Gho, M. Suh, and C. Kang. 2002. Highly sensitive and fast protein detection with Coomassie brilliant blue in sodium dodecyl sulfate-polyacrylamide gel clectrophoresis. Bull. Korean Chem. Soc. 23:1511-1512.
8. Karlinsay, J. E., J. Lonner, K. L. Brown, and K. T. Hughes. 2000. Translation/secretion coupling by type III secretion systems. Cell 102:487-497.
9. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 33:267-272.
10. LaVallie, E. R., and M. L. Stahl. 1989. Cloning of the flagellin gene from Bacillus subtilis and complementation studies of an in vitro-derived deletion mutation. J. Bacteriol. 171:3085-3094.
11. Majander, K., L. Anton, J. Antikainen, H. Lång, M. Brummer, T. K. Korhonen, and B. Westerlund-Wikström, 2005. Extracellular secretion of polypeptides using a modified Escherichia coli flagellar secretion apparatus. Nat. Biotechnol. 23:475-481.
12. Poncet, S., C. Bernard, E. Dervyn, J. Cayley, A. Klier, and G. Rapoport. 1997. Improvement of Bacillus sphaericus against dipteran larvae by integration, via homologous recombination, of the Cry 11A toxin gene from Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 63:4413-4420.
13. Stephenson, K., S. Bron, and C. R. Harwood. 1999. Cellular lysis in Bacillus subtilis: the effect of multiple extracellular protease deficiencies. Lett. Appl. Microbiol. 29:141-145.
14. Wu, S.-C., J. C. Yeung, Y. Duan, R. Ye, S. J. Szarka, H. R. Habibi, and S.-L. Wong. 2002. Functional production and characterization of a fibrin-specific single-chain fragment from Bacillus subtilis: effects of molecular chaperones and a wall-bound protease on antibody fragment production. Appl. Environ. Microbiol. 68:3261-3269.