cDNA amplification by SMART-PCR and suppression subtractive hybridization (SSH)-PCR

Methods in Molecular Biology

Volumn 496, Issue , 2009, Pages 223-243

  Hillmann, Andrew ^a   Dunne, Eimear ^b   Kenny, Dermot ^b  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^b ROYAL COLLEGE OF SURGEONS IN IRELAND   (Ireland)

Author keywords

cDNA amplification; Differential expression; SMART PCR; Subtracted cDNA; Subtractive hybridization; Suppressive PCR

Indexed keywords

COMPLEMENTARY DNA; RNA;

ANIMAL; GENE LIBRARY; GENETICS; HUMAN; METHODOLOGY; NUCLEIC ACID HYBRIDIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW;

ANIMALS; DNA, COMPLEMENTARY; GENE LIBRARY; HUMANS; NUCLEIC ACID HYBRIDIZATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA;

EID: 58149312869     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-553-4_15     Document Type: Article

References (8)

1. Chenchik, A., Zhu, Y. Y., Diatchenko, L., et al. (1998) Generation and use of high-quality cDNA from small amounts of total RNA by SMART PCR, in Gene Cloning and Analysis by RT-PCR. BioTechniques Books, MA. 305-319.
2. Chenchik, A., Moqadam, F., Siebert, P. (1996) A new method for full-length cDNA cloning by PCR, in A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis. Wiley-Liss, Inc. 273-321.
3. Wetmur, J. G., Davidson, N. (1968) Kinetics of renaturation of DNA. J Mol Biol 31, 349-370.
4. Ji, W., et al. (2002) Efficacy of SSH PCR in isolating differentially expressed genes. BMC Genomics 3, 12.
5. Diatchenko, L., et al. (1996) Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci USA 93, 6025-6030.
6. Hillmann, A. G., et al. (2006) Comparative RNA expression analyses from smallscale, single-donor platelet samples. J Thromb Haemost 4, 349-356.
7. CLONTECH Laboratories, P.A., CA, USA, PCR-Select cDNA Subtraction kit Kit User Manual. 2007.
8. CLONTECH Laboratories, P.A., CA, USA, SMART PCR cDNA SYNTHESIS Kit User Manual. 2007.