RNA profiling in peripheral blood cells by fluorescent differential display PCR

Methods in Molecular Biology

Volumn 496, Issue , 2009, Pages 211-222

  Steinau, Martin ^a   Rajeevan, Mangalathu S ^a  

^a NATIONAL CENTER FOR EMERGING AND ZOONOTIC INFECTIOUS DISEASES   (United States)

Author keywords

Blood RNA profiling; Differential display PCR; Fluorescent detection; PBMC

Indexed keywords

RNA;

ANIMAL; BLOOD; CYTOLOGY; DNA MICROARRAY; FLUORESCENCE; GENE EXPRESSION PROFILING; GENETICS; HUMAN; METHODOLOGY; MONONUCLEAR CELL; POLYMERASE CHAIN REACTION; REVIEW;

ANIMALS; FLUORESCENCE; GENE EXPRESSION PROFILING; HUMANS; LEUKOCYTES, MONONUCLEAR; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYMERASE CHAIN REACTION; RNA;

EID: 58149293470     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-553-4_14     Document Type: Article

References (6)

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2. Liang, P., Pardee, A. B. (1997) Differential display. A general Protocol. Methods Mol Biol 85, 3-11.
3. Ranamukhaarachchi, D. G., Rajeevan, M. S., Vernon, S. D., Unger, E. R. (2002) Modifying differential display polymerase chain reaction to detect relative changes in gene expression profiles. Anal Biochem 306, 343-346.
4. Steinau, M., Unger, E. R., Vernon, S. D., Jones, J. F., Rajeevan, M. S. (2004) Differential-display of peripheral blood for biomarker discovery in chronic fatigue syndrome. J Mol Med 82, 750-755.
5. Rajeevan, M. S., Ranamukhaarachchi, D. G., Vernon, S. D., Unger, E. R. (2001) Use of realtime quantitative PCR to validate the results of cDNA Array and differential display PCR technologies. Methods 25, 443-451.
6. Genomyx Corporation (1997). FluoroDD TMR-fluorescent anchored primer adaptor kit for the HIEROGLYPH mRNA Profile Kit System for Differential Display Analysis. Application Manual. Foster City, CA.