An efficient regeneration from nodal explants of Withania somnifera dunal

Asian Journal of Plant Sciences

Volumn 7, Issue 6, 2008, Pages 551-556

  Sivanesan, Iyyakkannu ^a,b   Murugesan, K ^a  

^a UNIVERSITY OF MADRAS   (India)

^b Gyeongsang National University   (South Korea)

Author keywords

Axillary shoot multiplication; B5 medium; Direct regeneration; Medicinal plant; Schenk and hilderbrandt

Indexed keywords

WITHANIA SOMNIFERA;

EID: 58149220829     PISSN: 16823974     EISSN: 18125697     Source Type: Journal    
DOI: 10.3923/ajps.2008.551.556     Document Type: Article

References (26)

1. Amutha, S., A. Ganapathi and M. Muruganantham, 2003. In vitro organogenesis and plant formation in Vigna radiata (L.). Wilczek. Plant Cell Tissue Org. Cult., 72: 203-207.
2. Devi, P.U., A.C. Sharada, F.E. Solomon and M.S. Kamath, 1992. In vivo growth inhibitory effect of Withania somnifera (Ashwagandha) on a transplantable mouse tumour, Sarcoma 180. Indian J. Exp. Biol., 30: 169-172.
3. Devi, P.U., 1996. Withania somnifera Dunal. (Ashwagandha): Potential plant source of a promising drug for cancer chemotherapy and radio sensitization. Indian J. Exp. Biol., 34: 927-932.
4. Gamble, J.S., 1918. Flora of the Presidency of Madras 2. Botanical Survey of India, Calcutta, India.
5. Gamborg, O.L., R.A. Miller and K. Ojima, 1968. Nutrient requirement of suspension cultures of soybean root cells. Exp. Cell Res., 50: 151-158.
6. Handique, P.J. and P. Bora, 1999. In vitro regeneration of a medicinal plant Houttuynia cordata Thunb. from nodal explants. Curr. Sci., 76: 1245-1247.
7. Inomata, S., M. Yokoyama, Y. Gozu, T. Shimizu and M. Yanagi, 1993. Growth pattern and ginsenoside production of Agrobacterium-transformed Panax ginseng roots. Plant Cell Rep., 12: 681-686.
8. Kiritikar, K.R. and B.D. Basu, 1975. Indian Medicinal Plants 3. 1st Edn., Bishen Singh Mahendra Pal Sing, Dehra Dun.
9. Kukreja, A.K. and A.K. Mathur, 1985. Tissue culture studies in Duboisia myoporoides; 1. Plant regeneration and clonal propagation by stem node cultures. Planta Med., 51: 93-96.
10. Kulkarni, A.A., S.R. Thangane and K.V. Krishnamoorthy, 1999. Withania somnifera (L.) Dunal. In: Biotechnological Applications of Plant Tissue and Cell Culture. Ravishankar, G.A. (Ed.). Oxford, IBH, India, pp: 156-159.
11. Kulkarni, A.A., S.R. Thangane and K.V. Krishnamoorthy, 2000. Direct shoot regeneration from node, internode, hypocotyl and embryo explants of W. somnifera. Plant Cell Tissue Org. Cult., 62: 203-209.
12. Manickam, V.S., R.E. Mathavan and R. Antonisamy, 2000. Regeneration of Indian ginseng plantlets from stem callus. Plant Cell Tissue Org. Cult., 62: 181-185.
13. Marin, M.L. and J.A. Marin, 1998. Excised rootstock roots cultured in vitro. Plant Cell Rep., 18: 350-355.
14. Mathur, J., P.S. Ahuja, A. Mathur, A.K. Kukreja and N.C. Shah 1988. In vitro propagation of Valeriana wallichii. Planta Med., 54: 82-83.
15. Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol., 15: 473-497.
16. Onay, A., 2000. Micropropagation of Pistachio from mature trees. Plant Cell Tissue Org. Cult., 60: 159-163.
17. Rani, G. and I.S. Grover, 1999. In vitro callus induction and regeneration studies in W. somnifera. Plant Cell Tissue Org. Cult., 57: 23-27.
18. Salvi, N.D., H. Singh, S. Tivarekar and S. Eapen, 2001. Plant regeneration from different expiants of neem. Plant Cell Tissue Org. Cult., 65: 159-162.
19. Schenk, R.U. and A.C. Hildebrandt, 1972. Medium and techniques for induction and growth of monocotyledons and dicotyledonous plant cell cultures. Can. J. Bot., 50: 199-204.
20. Singh, N.K. and C.B. Sehgal, 1999. Micropropagation of Holy Basil (Ocimum sanctum Linn.) from young inflorescences of mature plants. Plant Growth Regul., 29: 161-166.
21. Sivanesan, I. and K. Murugesan, 2005. In vitro adventitious shoot formation from leaf explants of Withania somnifera Dunal. Plant Cell Biotechnol. Mol. Biol., 6: 163-166.
22. Sivanesan, I., 2007a. Direct regeneration from apical bud explants of Withania somnifera Dunal. Indian J. Biotechnol., 16: 125-127.
23. Sivanesan, I., 2007b. Shoot regeneration and somaclonal variation from leaf callus cultures of Plumbago zeylanica Linn. Asian J. Plant Sci., 6: 83-86.
24. Sivanesan, I. and B.R. Jeong, 2007a. Micropropagation and in vitro flowering in Pentanema indicum Ling. Plant Biotechnol., 24: 527-532.
25. Sivanesan, I. and B.R. Jeong, 2007b. Direct shoot regeneration from nodal explants of Sida cordifolia Linn. In vitro Cell Dev. Biol. Plant, 43: 436-441.
26. Tivarekar, S. and S. Eapen, 2001. High frequency plant regeneration from immature cotyledons of mungbean. Plant Cell Tissue Organ Cult., 66: 227-230.