Identification of the lysine residue responsible for coenzyme A binding in the heterodimeric 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus tokodaii, a thermoacidophilic archaeon, using 4-fluoro-7-nitrobenzofurazan as an affinity label

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1794, Issue 2, 2009, Pages 335-340

  Luo, Jing ^a   Fukuda, Eriko ^a   Takase, Hirofumi ^a   Fushinobu, Shinya ^a   Shoun, Hirofumi ^a   Wakagi, Takayoshi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

2 Oxoacid:ferredoxin oxidoreductase; Affinity label; Archaea; CoA; NBD F; Sulfolobus

Indexed keywords

4 FLUORO 7 NITROBENZOFURAZAN; COENZYME A; FERREDOXIN 2 OXOACID REDUCTASE; FURAZAN DERIVATIVE; LYSINE; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SULFOLOBUS TOKODAII; THERMOACIDOPHILIC ARCHAEON;

4-CHLORO-7-NITROBENZOFURAZAN; AFFINITY LABELS; AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; BINDING SITES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COENZYME A; KETONE OXIDOREDUCTASES; LYSINE; METALLOENDOPEPTIDASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN MULTIMERIZATION; PROTEIN SUBUNITS; SULFOLOBUS;

ARCHAEA; SULFOLOBUS; SULFOLOBUS TOKODAII;

EID: 58149204083     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2008.10.006     Document Type: Article

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