Single sample extraction protocol for the quantification of NAD and NADH redox states in Saccharomyces cerevisiae

Journal of Separation Science

Volumn 31, Issue 18, 2008, Pages 3202-3211

  Sporty, Jennifer L ^a   Kabir, Md Mohiuddin ^a   Turteltaub, Kenneth W ^a   Ognibene, Ted ^a   Lin, Su Ju ^b   Bench, Graham ^a  

^a LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

HPLC; Isotope label; NAD; NADH; Yeast

Indexed keywords

AMIDES; AMMONIUM COMPOUNDS; CENTRIFUGATION; EXTRACTION; GLUCOSE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPES; METABOLISM; METABOLITES; ORGANIC SOLVENTS; YEAST;

AMMONIUM ACETATES; CELL LYSATES; CHLOROFORM EXTRACTIONS; EXTRACTION PROTOCOLS; HPLC; ISOTOPE LABELING; LIQUID MEDIAS; METABOLIC PATHWAYS; NAD; NADH; NICOTINAMIDE ADENINE DINUCLEOTIDE; PROTEIN DENATURATIONS; REDOX STATE; RELATIVE CONTRIBUTIONS; SACCHAROMYCES CEREVISIAE; SIMULTANEOUS MEASUREMENTS; SINGLE SAMPLES; SUPERNATANTS; UV-VIS ABSORBANCE; YEAST CELLS;

CELL CULTURE;

AMMONIUM ACETATE; CHLOROFORM; GLUCOSE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NITROGEN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; CELL LYSATE; CENTRIFUGATION; CONTROLLED STUDY; CULTURE MEDIUM; FREEZE DRYING; FUNGAL METABOLISM; FUNGUS CULTURE; FUNGUS GROWTH; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; OXIDATION REDUCTION STATE; PRIORITY JOURNAL; PRODUCT RECOVERY; PROTEIN DENATURATION; QUANTITATIVE ANALYSIS; RELIABILITY; REPRODUCIBILITY; SACCHAROMYCES CEREVISIAE; SOLVENT EXTRACTION; SUPERNATANT; ULTRAVIOLET SPECTROSCOPY; YEAST CELL; CHEMISTRY; EVALUATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; OXIDATION REDUCTION REACTION; SENSITIVITY AND SPECIFICITY;

SACCHAROMYCES CEREVISIAE;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; NAD; OXIDATION-REDUCTION; REPRODUCIBILITY OF RESULTS; SACCHAROMYCES CEREVISIAE; SENSITIVITY AND SPECIFICITY;

EID: 58149174156     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.200800238     Document Type: Article

References (40)

1. Sandmeier, J. J., Celic, I., Boeke, J. D., Smith, J. S., Genetics 2002, 160, 877-889.
2. Smith, J. S., Boeke, J. D., Genes Dev. 1997, 11, 241-254.
3. Anderson, R. M., Bitterman, K. J., Wood, J. G., Medvedik, O., Cohen, H., Lin, S. S., Manchester, J. K., Gordon, J. I., Sinclair, D. A., J. Biol. Chem. 2002, 277, 18881-18890.
4. Lin, S. J., Defossez, P. A., Guarente, L., Science 2000, 289, 2126-2128.
5. Lin, S. J., Kaeberlein, M., Andalis, A. A., Sturtz, L. A., Defossez, P. A., Culotta, V. C., Fink, G. R., Guarente, L., Nature 2002, 418, 344-348.
6. Garcia Soriano, F., Virag, L., Jagtap, P., Szabo, E., Mabley, J. G., Liaudet, L., Marton, A., Hoyt, D. G., Murthy, K. G., Salzman, A. L., Southan, G. J., Szabo, C., Nat. Med. 2001, 7, 108-113.
7. Greenamyre, J. T., Sherer, T. B., Betarbet, R., Panov, A. V., IUBMB Life 2001, 52, 135-141.
8. Zhang, Q., Piston, D. W., Goodman, R. H., Science 2002, 295, 1895-1897.
9. Ashrafi, K., Lin, S. S., Manchester, J. K., Gordon, J. I., Genes Dev. 2000, 14, 1872-1885.
10. Lin, S. J., Ford, E., Haigis, M., Liszt, G., Guarente, L., Genes Dev. 2004, 18, 12-16.
11. Lin, S. S., Manchester, J. K., Gordon, J. I., J. Biol. Chem. 2001, 276, 36000-36007.
12. Smith, J. S., Brachmann, C. B., Celic, I., Kenna, M. A., Muhammad, S., Starai, V. J., Avalos, J. L., Escalante-Semerena, J. C., Grubmeyer, C., Wolberger, C., Boeke, J. D., Proc. Natl. Acad. Sci. USA 2000, 97, 6658-6663.
13. Nemoto, S., Fergusson, M. M., Finkel, T., Science 2004, 306, 2105-2108.
14. Anderson, R. M., Bitterman, K. J., Wood, J. G., Medvedik, O., Sinclair, D. A., Nature 2003, 423, 181-185.
15. Grubmeyer, C. T., Gross, J. W., Rajavel, M., Meth. Enzymol. 1999, 308, 28-48.
16. Belenky, P., Racette, F. G., Bogan, K. L., McClure, J. M., Smith, J. S., Brenner, C., Cell 2007, 129, 473-484.
17. Bieganowski, P., Brenner, C., Cell 2004, 117, 495-502.
18. Tempel, W., Rabeh, W. M., Bogan, K. L., Belenky, P., Wojcik, M., Seidle, H. F., Nedyalkova, L., Yang, T., Sauve, A. A., Park, H. W., Brenner, C., PLoS Biol. 2007, 5, 263.
19. Anderson, R. M., Latorre-Esteves, M., Neves, A. R., Lavu, S., Medvedik, O., Taylor, C., Howitz, K. T., Santos, H., Sinclair, D. A., Science 2003, 302, 2124-2126.
20. Turteltaub, K. W., Vogel, J. S., Curr. Pharm. Des. 2000, 6, 991-1007.
21. Klaidman, L. K., Leung, A. C., Adams, J. D., Jr., Anal. Biochem. 1995, 228, 312-317.
22. Lazzarino, G., Amorini, A. M., Fazzina, G., Vagnozzi, R., Signoretti, S., Donzelli, S., Di Stasio, E., Giardina, B., Tavazzi, B., Anal. Biochem. 2003, 322, 51-59.
23. Pogolotti, A. L., Jr., Santi, D. V., Anal. Biochem. 1982, 126, 335-345.
24. Evans, J., Wang, T. C., Heyes, M. P., Markey, S. P., Anal. Biochem. 2002, 306, 197-203.
25. Qin, W., Yang, T., Ho, L., Zhao, Z., Wang, J., Chen, L., Zhao, W., Thiyagarajan, M., MacGrogan, D., Rodgers, J. T., Puigserver, P., Sadoshima, J., Deng, H., Pedrini, S., Gandy, S., Sauve, A. A., Pasinetti, G. M., J. Biol. Chem. 2006, 281, 21745-21754.
26. Sauve, A. A., Moir, R. D., Schramm, V. L., Willis, I. M., Mol. Cell 2005, 17, 595-601.
27. Yang, H., Yang, T., Baur, J. A., Perez, E., Matsui, T., Carmona, J. J., Lamming, D. W., Souza-Pinto, N. C., Bohr, V. A., Rosenzweig, A., de Cabo, R., Sauve, A. A., Sinclair, D. A., Cell 2007, 130, 1095-1107.
28. Sherman, F., Fink, G. R., Lawrence, C. W., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 1978.
29. Sherman, F., Meth. Enzymol. 2002, 350, 3-41.
30. Navas, P., Villalba, J. M., de Cabo, R., Mitochondrion 2007, 7, S34-S40.
31. Kaeberlein, M., Andalis, A. A., Fink, G. R., Guarente, L., Mol. Cell Biol. 2002, 22, 8056-8066.
32. Rover Junior, L., Fernandes, J. C., de Oliveira Neto, G., Kubota, L. T., Katekawa, E., Serrano, S. H., Anal. Biochem. 1998, 260, 50-55.
33. Lin, S. J., Guarente, L., Curr. Opin. Cell Biol. 2003, 15, 241-246.
34. Gaikwad, A., Long, D. J., II, Stringer, J. L., Jaiswal, A. K., J. Biol. Chem. 2001, 276, 22559-22564.
35. MacDonald, M. J., Marshall, L. K., Arch. Biochem. Biophys. 2000, 384, 143-153.
36. Mongan, P. D., Capacchione, J., West, S., Karaian, J., Dubois, D., Keneally, R., Sharma, P., Am. J. Physiol. Heart Circ. Physiol. 2002, 283, H1634-H1644.
37. Ramasamy, R., Trueblood, N., Schaefer, S., Am. J. Physiol. 1998, 275, H195-H203.
38. Sanni, L. A., Rae, C., Maitland, A., Stocker, R., Hunt, N. H., Am. J. Pathol. 2001, 159, 1105-1112.
39. Chang, J. Y., J. Biol. Chem. 1999, 274, 123-128.
40. Vogel, J. S., Palmblad, N. M., Ognibene, T., Kabir, M. M., Buchholz, B. A., Bench, G., Nucl. Instrum. Methods Phys. Res., Sect. B 2007, 259, 745-751.