Suppression of cardiac troponin T induces reduction of contractility and structural disorganization in chicken cardiomyocytes

Cell Structure and Function

Volumn 33, Issue 2, 2008, Pages 193-201

  Toyota, Naoji ^a   Takano Ohmuro, Hiromi ^b   Yoshida, Lucia S ^b   Araki, Masatake ^c   Yoshinobu, Kumiko ^c   Suzuki Toyota, Fumie ^d  

^a KUMAMOTO GAKUEN UNIVERSITY   (Japan)

^b MUSASHINO UNIVERSITY   (Japan)

^c KUMAMOTO UNIVERSITY   (Japan)

^d CHIBA UNIVERSITY   (Japan)

Author keywords

Beating; Intercalated disks; Primary culture; RNAi; Z Z intervals

Indexed keywords

ALPHA ACTININ; CONNECTIN; DOUBLE STRANDED RNA; MYOSIN; SMALL INTERFERING RNA; TROPOMYOSIN; TROPONIN I; TROPONIN T;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL COMMUNICATION; CELL ULTRASTRUCTURE; CONTROLLED STUDY; EMBRYO; EMBRYO CULTURE; GENETIC TRANSFECTION; HEART BEAT; HEART CELL CULTURE; HEART MUSCLE CELL; HEART MUSCLE CONTRACTILE FORCE; HEART MUSCLE CONTRACTILITY; IMMUNOBLOTTING; MUSCLE FIBRIL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN SYNTHESIS INHIBITION; PROTEIN SYNTHESIS REGULATION; SKELETAL MUSCLE; STRUCTURAL HOMOLOGY;

ANIMALS; BASE SEQUENCE; CELL ADHESION; CELLS, CULTURED; CHICKENS; GENE EXPRESSION REGULATION; MYOCARDIAL CONTRACTION; MYOCYTES, CARDIAC; RNA, SMALL INTERFERING; SARCOMERES; SEQUENCE HOMOLOGY, NUCLEIC ACID; SUPPRESSION, GENETIC; TRANSFECTION; TROPONIN T;

EID: 58149158048     PISSN: 03867196     EISSN: 13473700     Source Type: Journal    
DOI: 10.1247/csf.08010     Document Type: Article

References (19)

1. DeHaan, R.L. 1970. The potassium-sensitivity of isolated embryonic heart cells increases with development. Dev. Biol., 23: 226-240.
2. DeHaan, R.L. and Hirakow, R. 1972. Synchronization of pulsation in isolated cardiac myocytes. Exp. Cell Res., 70: 214-220.
3. Ebashi, S. 1961. Calcium binding activity of vesicular relaxing factor. J. Biochem., 50: 236-244.
4. Gomes, A.V., Barnes, J.A., Harada, K., and Potter, J.D. 2004. Role of troponin T in disease. Mol. Cell Biochem., 263: 115-129.
5. Kamisago, M., Sharma, S.D., DePalma, S.R., Solomon, S., Sharma, P., McDonough, B., Smoot, L., Mullen, M., Woolf, P., Wigle, E.D., Seidman, J.G., and Seidman, C.E. 2000. Mutations in sarcomere protein genes as a cause of dilated cardiomyopathy. N. Engl. J. Med., 343: 1688-1696.
6. Kostin, S. and Schaper, J. 2001. Tissue-specific patterns of gap junctions in adult rat atrial and ventricular cardiomyocytes in vitvo and in vitro. Circ. Res., 88: 933-939.
7. Lombardi, R., Bell, A., Senthil, V., Sidhu, J., Noseda, M., Roberts, R., and Marian, A.J. 2008. Differential interactions of thin filament proteins in two cardiac troponin T mouse models of hypertrophic and dilated cardiomyopathies. Cardiovasc. Res., 79: 109-117.
8. Miller, G., Musa, H., Gautel, M., and Peckham, H. 2003. A targeted deletion of the C-terminal end of titin, including the titin kinase domain, impairs myofibrillogenesis. J. Cell Sci., 116: 4811-4819.
9. Muir, A.R. 1957. An electron microscope study of the embryology of the intercalated disc in the heart of the rabbit. J. Biophys. Biochem. Cytol., 25: 193-202.
10. Perry, S.V. 1998. Troponin T: genetics, proteins and function. J. Muscle Res. Cell Motil., 19: 575-602.
11. Sehnert, A.J., Huq, A., Weinstein, B.M., Walker, C., Fishman, M., and Stainier, D.Y. 2002. Cardiac troponin T is essential in sarcomere assembly and cardiac contractility. Nat Genet, 31: 106-110.
12. Shimizu, N. and Shimada, Y. 1985. Immunochemical analysis of troponin T isoforms in adult, embryonic, regenerating and denervated chicken fast skeletal muscle. Dev. Biol., 111: 324-334.
13. Tanaka, H., Suzuki, H., Ohtsuki, I., and Ojima, T. 2008. Structure-function relationships of molluscan troponin T revealed by limited proteolysis. Biochem. Biophys. Acta., 1784: 1037-1042.
14. Tardiff, J.C., Hewett, T.E., Palmerm, B.M., Olsson, C., Factor, S.M., Moore, R.L., Robbins, J., and Leinwand, L.A. 1999. Cardiac troponin T mutations result in allele-specific phenotypes in a mouse model for hypertrophic cardiomyopathy. J. Clin. Invest., 104: 469-481.
15. Tokuyasu, K.T., Dutton, Anne H., and Singer, S.J. 1983. Immunoelectron microscopic studies of desmin (skeletin) localization and intermediate filament organization in chicken cardiac muscle. J. Cell Biol., 96: 1736-1742.
16. Toyota, N. and Shimada, Y. 1981. Differentiation of troponin in cardiac and skeletal muscles in chicken embryos as studied by immunofluorescence. J. Cell Biol., 91: 487-504.
17. Toyota, N. and Shimada, Y. 1983. Isoform variants of troponin in skeletal and cardiac muscle cells cultured with and without nerves. Cell, 33: 297-304.
18. Toyota, N., Uzawa, H., and Shimada, Y. 1998. Assembly of force-expressed troponin I isoforms in myofibrils of cultured cardiac and fast skeletal muscle cells as studied by epitope tagging. J. Muscle Res. Cell Motil., 19: 937-947.
19. Watkins, H., McKenna, W.J., Thierfelder, L., Suc, H.J., Anan, R., Donoghue, A., Spirito, P., Matsumori, A., Moravec, C.S., Seidman, J.G., and Seidman. C.E. 1995. Mutations in the genes for cardiac troponin T and alpha-tropomyosin in hypertrophic cardiomyopathy. N. Engl. J. Med., 332: 1058-1064.