Protamine inhibits formation of the covalent factor IXa-anti-thrombin complex

Blood Coagulation and Fibrinolysis

Volumn 19, Issue 6, 2008, Pages 591-596

  Brecher, Arthur S ^a   Roland, Amie R ^a  

^a BOWLING GREEN STATE UNIVERSITY   (United States)

Author keywords

Antithrombin; Coagulation; Factor IXa; Protamine sulfate; SDS polyacrylamide gel electrophoresis

Indexed keywords

ANTICOAGULANT AGENT; ANTITHROMBIN; BLOOD CLOTTING FACTOR 9A; HEPARIN; PROCOAGULANT; PROTAMINE SULFATE; ANTITHROMBIN III; HEPARIN ANTAGONIST; PROTAMINE;

ANTICOAGULATION; ARTICLE; AUTOLYSIS; BLOOD CLOTTING; CONTROLLED STUDY; DRUG EFFECT; DRUG PROTEIN BINDING; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; QUANTITATIVE ANALYSIS; CHEMICAL PHENOMENA; HUMAN; METABOLISM; PROTEIN BINDING; PROTEIN ELECTROPHORESIS; WESTERN BLOTTING;

ANTITHROMBIN III; BLOOD PROTEIN ELECTROPHORESIS; BLOTTING, WESTERN; DEPRESSION, CHEMICAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FACTOR IXA; HEPARIN ANTAGONISTS; HUMANS; MOLECULAR WEIGHT; PROTAMINES; PROTEIN BINDING;

EID: 58149107963     PISSN: 09575235     EISSN: None     Source Type: Journal    
DOI: 10.1097/MBC.0b013e328308917c     Document Type: Article

References (11)

1. Jacques LB, Kavanagh LW. Protamine neutralization factors for heparin. Can J Pharm Sci 1977; 12:44-47.
2. Perkash A. A comparison of the quantitative action of protamine and heparin on blood coagulation. Am J Clin Pathol 1980; 73:676-681.
3. Brecher AS, Hommema EL, Rasmusson E, Adamu ET, Coggin MH, Nagy ZM. Nanomoles of protamine sulfate accelerate catalysis by thrombin [abstract 0-HM-147]. Central Soc Clin Res 1999; 16-18 September; Chicago, IL.
4. Brecher AS, HommemaEL (2001). The influenceof protamine sulfate upon the interaction between thrombin and antithrombin III. Proceedings of the International Conference on Biochemical and Molecular-biological Aspects of the Brain Immune System; 15-19 September, 2001; Yerevan Tsakhkadzor, Armenia. pp. 74-81.
5. Coggin MH, Ahl R, Roland A, Brecher AS (2002). The influence of protamine sulfate and heparin upon the interaction of Factor Xa with antithrombin III [abstract #2089]. Experimental Biology 2002, 20-24 April; New Orleans, LA.
6. Coggin MH (2003). The effects of several biologically active peptides on factors of the blood coagulation cascade [MS thesis]. Bowling Green (OH): Bowling Green State University.
7. Laemmli UK. Cleavage of the structural protein during assembly of the head of bacteriophage T4. Nature 1970; 227:680-685.
8. Gravel P, Golaz O. Protein blotting by the semi-dry method. In: Walker JM, editor. The protein protocols handbook. Totowa, NJ: Humana Press; 1996. pp. 249-260.
9. Olson ST, Bjork I, Shore JD. Kinetic characterization of heparin-catalyzed and uncatalyzed inhibition of blood coagulation proteinases by antithrombin. Methods Enzymol 1993; 222:525-559.
10. Nossell NL, Rubin H, Drillings M, Hsieh R. Inhibition of Hageman factor activation. J Clin Invest 1968; 47:1172-1180.
11. Cobel-Geard RJ, Hassouna HI. Interaction of protamine sulfate with thrombin. Am J Hematol 1983; 14:227-233.