Investigating recombinant protein exudation from roots of transgenic tobacco

Environmental Biosafety Research

Volumn 7, Issue 4, 2008, Pages 219-226

  Pizzuti, Francesca ^a   Daroda, Lorenza ^a  

^a ENEA C R CASACCIA   (Italy)

Author keywords

Biosafety; Hydroponic culture; KDEL; Plant molecular farming; Recombinant antibody; Rhizosecretion; Transgenic tobacco

Indexed keywords

PLANTIBODY; RECOMBINANT PROTEIN;

ARTICLE; METABOLISM; PHYSIOLOGY; PLANT EXUDATE; PLANT LEAF; PLANT ROOT; PLANT STEM; SECRETION; TOBACCO; TRANSGENIC PLANT;

PLANT EXUDATES; PLANT LEAVES; PLANT ROOTS; PLANT STEMS; PLANTIBODIES; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; TOBACCO;

NICOTIANA BENTHAMIANA; NICOTIANA TABACUM; PHASEOLUS VULGARIS; TOMATO SPOTTED WILT VIRUS; TOSPOVIRUS;

EID: 58149107303     PISSN: 16357922     EISSN: 16357930     Source Type: Journal    
DOI: 10.1051/ebr:2008020     Document Type: Article

References (36)

1. Arcalis E, Marcel S, Altmann F, Kolarich D, Drakakaki G, Fischer R, Christou P, Stoger E (2004) Unexpected deposition patterns of recombinant proteins in post-endoplasmic reticulum compartments of wheat endosperm. Plant Physiol. 136: 3457-3466
2. st century and the role of plants and plant cells as production platforms. Ann. N. Y. Acad. Sci. 1102: 121-134
3. Bradford MM (1976) A rapid and sensitive method for the quantification of micrograms quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72: 248-254
4. Commandeur U, Twyman M, Fisher R (2003) The biosafety of molecular farming in plants. AgBiotechNet 5: 1-9
5. Drakakaki G, Marcel S, Arcalis E, Altmann F, Gonzalez-Melendi P, Fischer R, Christou P, Stoger E (2006) The intracellular fate of a recombinant protein is tissue dependent. Plant Physiol. 141: 578-586
6. Elbehri A (2005) Biopharming and the food system: examining the potential benefits and risks. Agbioforum 8: 18-25
7. Faye L, Boulaflous A, Benchabane M, Gomord V, Michaud D (2005) Protein modifications in the plant secretory pathway: current status and practical implications in molecular pharming. Vaccine 23: 1770-1778
8. Floss DM, Falkenburg D, Conrad U (2007) Production of vaccines and therapeutic antibodies for veterinary applications in transgenic plants: an overview. Transgenic res. 16: 315-332
9. Franconi R, Roggero P, Pirazzi P, Arias FJ, Desiderio A, Bitti O, Pashkoulov D, Mattei B, Bracci L, Masenga V, Milne RG, Benvenuto E (1999) Functional expression in bacteria and plants of an scFv antibody fragment against tospoviruses. Immunotechnology 4: 189-201
10. Jurgens G (2004) Membrane traffiking in plants. Annu. Rev. Cell Dev. Biol. 20: 481-504
11. Kermode AR (2006) Plants as factories for the production of biopharmaceutical and bioindustrial proteins: lessons from cell biology. Can. J. Bot. 84: 679-694
12. Komarnytsky S, Borisjuk N, Yakoby N, Garvey A, Raskin I (2006) Cosecretion of protease inhibitor stabilize antibodies produced by plant roots. Plant Physiol. 141: 1185-1193
13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
14. Liénard D, Sourrouille C, Gomord V, Faye L (2007) Pharming and transgenic plants. Biotechnol. Annu. Rev. 13: 115-147
15. Lilley AK, Bailey MJ, Cartwright C, Turner SL, Hirsch PR (2006) Life in earth: the impact of GMplants on soil ecology? Trends Biotechnol. 24: 9-14
16. Munro S, Pelham HR (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48: 899-907
17. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473-497
18. Petruccelli S, Otegui MS, Lareu F, Tran Dinh O, Fitchette AC, Circosta A, Rumbo M, Bardor M, Carcamo R, Gomord V, Beachy RN (2006) A KDEL-tagged monoclonal antibody is efficiently retained in the endoplasmic reticulum in leaves, but is both partially secreted and sorted to protein storage vacuoles in seed. Plant Biotechnol. J. 4: 511-527
19. Pujol M, Gavilondo J, Ayala M, Rodrìguez M, Gonzàlez EM, Pèrez L (2007) Fighting cancer with plant-expressed pharmaceuticals. Trends Biotechnol. 25: 455-459
20. Ramessar K, Peremerti A, Gomez-Galera S, Naqvi S, Moralejo M, Munoz P, Capell T, Christou P (2007) Biosafety and risk assessment framework for selectable marker genes in transgenic crop plants: a case of the science not supporting the politics. Transgenic Res. 16: 261-280
21. Sabharwal N, Icoz I, Saxena D, Stotzky G (2007) Release of the recombinant proteins, human serum albumin, beta-glucuronidase, glycoprotein B from human cytomegalovirus, and green fluorescent protein, in root exudates from transgenic tobacco and their effects on microbes and enzymatic activities in soil. Plant Physiol. Biochem. 45: 464-469
22. Saint-Jore-Dupas C, Faye L, Gomord V (2007) From planta to pharma with glycosylation in the toolbox. Trends Biotechnol. 25: 317-323
23. Sanvido O, Romeis J, Bigler F (2007) Ecological impacts of genetically modified crops: ten years of field research and commercial cultivation. Adv. Biochem. Eng. Biotechnol. 107: 235-278
24. Saxena D, Flores S, Stotzky G (2002) Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events. Soil Biol. Biochem. 34: 133-137
25. Saxena D, Stewart CN, Altosaar I, Shu Q, Stotzky G (2004) Larvicidal Cry proteins from Bacillus thuringensis are released in root exudates of transgenic B. thuringensis corn, potato and rice but not of B. thuringensis canola, cotton and tobacco. Plant Physiol. Biochem. 42: 383-387
26. Sparrow PAC, Irwin JA, Dale PJ, Twyman RM, Ma J-KC (2007) Pharma-Planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals. Transgenic Res. 16: 147-161
27. Spök A (2007) Molecular farming on the rise-GMO regulators still walking a tightrope. Trends Biotechnol. 25: 74-82
28. Streatfield SJ (2007) Approaches to achieve high-level heterologous protein production in plants. Plant Biotechnol. J. 5: 2-15
29. Toubart P, Desiderio A, Salvi G, Cervone F, Daroda L, De Lorenzo G (1992) Cloning and characterization of the gene encoding the endopolygalacturonase-inhibiting protein (PGIP) of Phaseolus vulgaris L. Plant J. 2: 367-373
30. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc. Natl. Acad. Sci. USA 76: 4350-4353
31. Twyman RM, Schillberg S, Fischer R (2005) Transgenic plants in the biopharmaceutical market. Expert Opin. Emerg. Drugs 10: 185-218
32. Van Droogenbroeck B, Cao J, Stadlmann J, Altmann F, Colanesi S, Hillmer S, Robinson DG, Van Lerberge E, Terryn N, van Montagu M, Liang M, Depicker A, De Jaeger G (2007) Aberrant localization and underglycosylation of highly accumulating single-chain Fv-Fc antibodies in transgenic Arabidopsis seeds. Proc. Natl. Acad. Sci. USA 104: 1430-1435
33. van Esse HP, Thomma BPHJ, van't Klooster JW, de Wit PJGM (2006) Affinity-tags are removed from Cladosporium fulvum effector proteins expressed in the tomato leaf apoplast. J. Exp. Bot. 57: 599-608
34. Vitale A, Pedrazzini E (2005) Recombinant pharmaceuticals from plants: the plant endomembrane system as bioreactor. Mol. Interv. 5: 216-225
35. Widmer (2007) Assessing effects of transgenic crops on soil microbial communities. Adv. Biochem. Eng. Biotechnol. 107: 207-234
36. Wolt JD, Karaman S, Wang K (2007) Risk assessment for plant-made pharmaceuticals. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 2: 1-9