Expression and function analysis of the metallothionein-like (MT-like) gene from Festuca rubra in Chlamydomonas reinhardtii chloroplast

Science in China, Series C: Life Sciences

Volumn 51, Issue 12, 2008, Pages 1076-1081

  Han, SiHai ^a,b   Hu, ZhangLi ^a   Lei, AnPing ^a  

^a SHENZHEN UNIVERSITY   (China)

^b STATE KEY LABORATORY OF INDUSTRIAL CONTROL TECHNOLOGY   (China)

Author keywords

Cadmium binding properties; Chlamydomonas reinhardtii; Chloroplast; Metallothionein like (MT like) gene

Indexed keywords

CADMIUM; METALLOTHIONEIN;

ANIMAL; ARTICLE; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST; CYTOLOGY; FESCUE; GENETICS; METABOLISM; PHYSIOLOGY; TRANSGENIC ORGANISM;

ANIMALS; CADMIUM; CHLAMYDOMONAS REINHARDTII; CHLOROPLASTS; FESTUCA; METALLOTHIONEIN; ORGANISMS, GENETICALLY MODIFIED;

ALGAE; CHLAMYDOMONAS REINHARDTII; FESTUCA RUBRA;

EID: 58149094741     PISSN: 10069305     EISSN: 18622798     Source Type: Journal    
DOI: 10.1007/s11427-008-0136-3     Document Type: Article

References (16)

1. G. H. Wikforsl A. Neeman J. Paul 1991 Cadmium-binding polypeptides in microalgal strains with laboratory-induced cadmium tolerance Mar Ecol Prog Ser 79 163 170
2. J. L. Hall 2002 Celluar mechanisms for heavy metal detoxification and tolerance J Exp Bot 53 366 1 11
3. S. Siripornadulsil S. Traina D. P. Verma 2002 Molecular mechanisms of proline-mediated tolerance to toxic heavy metals in transgenic microalgae Plant Cell 14 2837 2847
4. C. Hu K. W. K. Lau M. Wu 2001 Cadmium sequestration in Chlamydomonas reinhardtii Plant Sci 161 987 996
5. X. X. Guo H. Zhao D. J. Shi 1998 Expression of mouse MT-I cDNA in filamentous cyanobacetium to enhance its metal-resistance Acta Bot Sin 40 4 320 324
6. L. Ren D. J. Shi J. X. Dai 1998 Expression of the mouse metallothionein-I gene conferring cadmium resistance in a transgenic cyanobacterium FEMS Microbiol Lett 158 1 127 132
7. Q. Shao D. J. Shi F. Y. Hao 2002 Cloning and expression of metallothionein mutant a-KKS-a in Anabaena sp. PCC7120 Mar Pollut Bull 45 163 167
8. W. L. Zeng F. F. Zhao Z. G. Cao 2008 Heavy metal tolerance of transgenic Synechococcus sp. PCC7002 with mouse metallothionein-I gene Environ Pollut Contr 30 30 66 70
9. X. H. Cai C. Brown J. Adhiya 1999 Growth and heavy metal binding properties of transgenic Chlamydomonas expressing a foreign met-allothionein gene Int J Phytoremediat 1 1 53 65
10. Hu Z L, Wong Y S. Expression of the metallothionein-like gene from Festuca rubra in Chlamydomomnas reinhardtii. Ninth International Conference on the Cell and Molecular Biology of Chlamydomonas, Amsterdam, 2000
11. Z. L. Hu M. Xing Y. H. Wu 2002 The comparison of heavy metal binding properties and tolerance between transgenic strains and wild type in Chlamydomonas reinhardtii J Lake Sci 14 3 247 252
12. J. E. Boynton N. W. Gillham E. H. Harris 1988 Chloroplast transformation Chlamydomonas with high velocity microprojectiles Science 240 1534 1538
13. M. Ma W. K. Tsang K. M. Kwan 1997 Preliminary studies of the identification and expression of metallothionein-like gene in Festuca rubra Acta Bot Sin 39 11 1078 1081
14. M. Ma W. K. Tsang P. S. Lau 1997 Cloning and sequencing of the metallothionein-like cDNA (Accession Number U96646) from Festuca rubra cv. Merlin (PGR 97-098) Plant Physiol 114 1136
15. Y. Nakamura T. Gojobori T. Ikemura 1999 Codon usage table from the international DNA sequence databases: its status Nucleic Acids Res 27 292
16. Luykx P. Minipreps of DNA from Chlamydomonas. http://www.chlamy.org/ methods.html