Circularly permutated bioluminescent probes for illuminating ligand-activated protein dynamics

Bioconjugate Chemistry

Volumn 19, Issue 12, 2008, Pages 2480-2486

  Sung, Bae Kim ^a   Sato, Moritoshi ^a   Tao, Hiroaki ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BIOLUMINESCENCE; ENZYME ACTIVITY; LIGANDS; MAMMALS; MOLECULAR DYNAMICS; MOLECULAR IMAGING; PLANTS (BOTANY); SIGNAL TO NOISE RATIO;

ACTIVATED PROTEINS; BIOACTIVES; C-TERMINUS; CIRCULAR PERMUTATION; CREATIVES; LIGAND BINDING DOMAIN; MOLECULAR EVENTS; N TERMINUS; PROTEIN DYNAMICS; SMALL MOLECULES;

PROBES;

4 HYDROXYTAMOXIFEN; CLICK BEETLE LUCIFERASE; ESTROGEN RECEPTOR; FIREFLY LUCIFERASE; GAUSSIA LUCIFERASE; LUCIFERASE; LUMINESCENT AGENT; PHOTOPROTEIN; PROTEIN SH2; UNCLASSIFIED DRUG;

ARTICLE; BEETLE; ENZYME ACTIVITY; FIREFLY; GAUSSIA PRINCEPS; LIGAND BINDING; MAMMAL CELL; MOLECULAR IMAGING; MOLECULAR PROBE; NONHUMAN; PHOTINUS PYRALIS; PLASMID; PYREARINUS TERMITILLUMINANS; SIGNAL NOISE RATIO;

GAUSSIA PRINCEPS (T. SCOTT, 1894); MAMMALIA; PHOTINUS PYRALIS; PYREARINUS TERMITILLUMINANS;

EID: 58149094136     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc800378a     Document Type: Article

References (24)

1. Brannigan, J. A., and Wilkinson, A. J. (2002) Protein engineering 20 years on. Nat. Rev. Mol. Cell Biol. 3, 964-970.
2. Weissleder, R., and Ntziachristos, V. (2003) Shedding light onto live molecular targets. Nat. Med. 9, 123-128.
3. Paulmurugan, R., Umezawa, Y., and Gambhir, S. S. (2002) Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies. Proc. Natl. Acad. Sci. U.S.A. 99, 15608-15613.
4. Paulmurugan, R., and Gambhir, S. S. (2005) Firefly luciferase enzyme fragment complementation for imaging in cells and living animals. Anal. Chem. 77, 1295-1302.
5. Paulmurugan, R., and Gambhir, S. S. (2006) An intramolecular folding sensor for imaging estrogen receptor-ligand interactions. Proc. Natl. Acad. Sci. U.S.A. 103, 15883-15888.
6. Remy, I., and Michnick, S. W. (2006) A highly sensitive protein-protein interaction assay based on Gaussia luciferase. Nat. Meth. 3, 977-979.
7. Kim, S. B., Ozawa, T., Watanabe, S., and Umezawa, Y. (2004) High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase. Proc. Natl. Acad. Sci. U.S.A. 101, 11542-11547.
8. Kim, S. B., Kanno, A., Ozawa, T., Tao, H., and Umezawa, Y. (2007) Nongenomic activity of ligands in the association of androgen receptor with Src. ACS Chem. Biol. 2, 484-492.
9. Kim, S. B., Umezawa, Y., Kanno, K. A., and Tao, H. (2008) An integrated-molecule-format multicolor probe for monitoring multiple activities of a bioactive small molecule. ACS Chem. Biol. 3, 359-372.
10. Kim, S. B., Otani, Y., Umezawa, Y., and Tao, H. (2007) Bioluminescent indicator for determining protein-protein interactions using intramolecular complementation of split click beetle luciferase. Anal. Chem. 79, 4820-4826.
11. Viviani, V. R., Uchida, A., Viviani, W., and Ohmiya, Y. (2002) The influence of Ala243 (Gly247), Arg215 and Thr226 (Asn230) on the bioluminescence spectra and pH-sensitivity of railroad worm, click beetle and firefly luciferases. Photochem. Photobiol. 76, 538-544.
12. 2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887.
13. Tannous, B. A., Kim, D. E., Fernandez, J. L., Weissleder, R., and Breakefield, X. O. (2005) Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol. Ther. 11, 435-443.
14. Varricchio, L., Migliaccio, A., Castoria, G., Yamaguchi, H., de Falco, A., Di Domenico, M., Giovannelli, P., Farrar, W., Appella, E., and Auricchio, F. (2007) Inhibition of estradiol receptor/Src association and cell growth by an estradiol receptor alpha tyrosine-phosphorylated peptide. Mol. Cancer Res. 5, 1213-1221.
15. Rich, R. L., Hoth, L. R., Geoghegan, K. F., Brown, T. A., LeMotte, P. K., Simons, S. P., Hensley, P., and Myszka, D. G. (2002) Kinetic analysis of estrogen receptor/ligand interactions. Proc. Natl. Acad. Sci. U.S.A. 99, 8562-8567.
16. Kim, S. B., Awais, M., Sato, M., Umezawa, Y., and Tao, H. (2007) Integrated molecule-format bioluminescent probe for visualizing androgenicity of ligands based on the intramolecular association of androgen receptor with its recognition peptide. Anal. Chem. 79, 1874-1880.
17. Baird, G. S., Zacharias, D. A., and Tsien, R. Y. (1999) Circular permutation and receptor insertion within green fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 96, 11241-11246.
18. Kawai, Y., Sato, M., and Umezawa, Y. (2004) Single color fluorescent indicators of protein phosphorylation for multicolor imaging of intracellular signal flow dynamics. Anal. Chem. 76, 6144-9.
19. 2+ indicators: using genetics and molecular design to understand complex physiology. J. Physiol. 578, 55-67.
20. Souslova, E. A., and Chudakov, D. M. (2007) Genetically encoded intracellular sensors based on fluorescent proteins. Biochemistry (Moscow, Russ. Fed.) 72, 683-697.
21. 2+ imaging by a new calmodulin-GFP fusion molecule. Nat. Struct. Biol. 8, 1069-1073.
22. Awais, M., Sato, M., Lee, X. F., and Umezawa, Y. (2006) A fluorescent indicator to visualize activities of the androgen receptor ligands in single living cells. Angew. Chem., Int. Ed. 45, 2707-2712.
23. Migliaccio, A., Castoria, G., Di Domenico, M., de Falco, A., Bilancio, A., Lombardi, M., Bottero, D., Varricchio, L., Nanayakkara, M., Rotondi, A., and Auricchio, F. (2002) Sex steroid hormones act as growth factors. J. Steroid Biochem. Mol. Biol. 83, 31-35.
24. Arnold, S. F., Melamed, M., Vorojeikina, D. P., Notides, A. C., and Sasson, S. (1997) Estradiol-binding mechanism and binding capacity of the human estrogen receptor is regulated by tyrosine phosphorylation. Mol. Endocrinol. 11, 48-53.