Rapid detection of vibrio harveyi in seawater by real-time PCR

Microbes and Environments

Volumn 23, Issue 2, 2008, Pages 172-176

  Fukui, Youhei ^a   Sawabe, Tomoo ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Quantitative real time PCR; Rapid detection; Vibrio harveyi

Indexed keywords

HALIOTIDAE; VIBRIO HARVEYI;

EID: 58049221413     PISSN: 13426311     EISSN: 13426311     Source Type: Journal    
DOI: 10.1264/jsme2.23.172     Document Type: Article

References (16)

1. Austin, B., and X-H. Zhang. 2006. Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Let. Appl. Microbiol. 43:119-124.
2. Bej, A.K. 2004. Detection of microbial nucleic acids by polymerase chain reaction in aquatic samples, p. 369-432. In G.A. Kowalchuk, F.J. de Bruijn, I.M. Head, A.D.L. Akkermans, and J.D. van Elsas (ed.), Molecular microbial ecology manual 2nd ed. Kluwer Academic, Netherlands.
3. Campbell, M.S., and A.C. Wright. 2003. Real-time PCR analysis of Vibrio vulnificus from oysters. Appl. Environ. Microbiol. 69:7137-7144.
4. Fukui, Y., and T. Sawabe. 2007. Improved one-step colony PCR detection of Vibrio harveyi. Microbes Environ. 22:1-10.
5. Gauger, E.J., and M. Gómez-Chiarri. 2002. 16S ribosomal DNA sequencing confirms the synonymy of Vibrio harveyi and V. carchariae. Dis. Aquat. Org. 52:39-46.
6. Gomez-Gil, B., F.L. Thompson, C.C. Thompson, and J. Swings. 2003. Vibrio rotiferianus sp. nov., isolated from cultures of the rotifer Brachionus plicatilis. Int. J. Syst. Evol. Microbiol. 53:239-243.
7. Gomez-Gil, B., S. Soto-Rodríguez, A. García-Gasca, A. Roque, R. Vazquez-Juarez, F.L. Thompson, and J. Swings. 2004. Molecular identification of Vibrio harveyi-related isolates associated with diseased aquatic organisms. Microbiology 150:1769-1777.
8. Higuchi, R., C. Fockler, G. Dollinger, and R. Watson. 1993. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 11:1026-1030.
9. Maidak, B.L., J.R. Cole, T.G. Lilburn, C.T. Parker Jr, P.R. Saxman, R.J. Farris, G.M. Garrity, G.J. Olsen, T.M. Schmidt, and J.M. Tiedje. 2001. The RDP-II (Ribosomal Database Project). Nucleic Acids Res. 29:173-174.
10. Sawabe, T., S. Inoue, Y. Fukui, K. Yoshie, Y. Nishihara, and H. Miura. 2007. Mass mortality of Japanese abalone Haliotis discus hannai caused by Vibrio harveyi infection. Microbes Environ. 22:300-308.
11. Sawabe, T., K. Kita-Tsukamoto, and F.L. Thompson. 2007. Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J. Bacteriol. 189:7932-7936.
12. Thaithongnum, S., P. Ratanama, K. Weeradechapol, A. Sukhoom, and V. Vuddhakul. 2006. Detection of V. harveyi in shrimp postlarvae and hatchery tank water by the most probable number technique with PCR. Aquaculture 261:1-9.
13. Thompson, F.L., B. Gomez-Gil, A.T.R. Vasconcelos, and T. Sawabe. 2007. Multilocus sequence analysis reveals that Vibrio harveyi and V. campbellii are distinct species. Appl. Environ. Microbiol. 73:4279-4285.
14. Thompson, F.L., I. Cleenwerck, J. Swings, J. Matsuyama, and T. Iida. 2007. Genomic diversity and homologous recombination in Vibrio parahaemolyticus as revealed by amplified fragment length polymorphism (AFLP) and multilocus sequence analysis (MLSA). Microbes Environ. 22:373-379.
15. Walsh, P.S., D.A. Metzger, and R. Higuchi. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506-513.
16. Ward, L.N., and A.K. Bej. 2006. Detection of Vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes. Appl. Environ. Microbiol. 72:2031-2042.