Expression, purification, and characterization of iron-sulfur cluster assembly regulator IscR from Acidithiobacillus ferroaxidans

Journal of Microbiology and Biotechnology

Volumn 18, Issue 10, 2008, Pages 1672-1677

  Zeng, Jia ^a   Zhang, Ke ^a   Liu, Jianshe ^a   Qiu, Guanzhou ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Acidithiobacillus ferrooxidans; Assembly; His tag; Iron sulfure cluster; Regulator

Indexed keywords

IRON SULFUR PROTEIN; SCAFFOLD PROTEIN;

ACIDITHIOBACILLUS FERROOXIDANS; AFFINITY CHROMATOGRAPHY; ARTICLE; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENE CLUSTER; GENE INSERTION; GENETIC TRANSCRIPTION; MOLECULAR WEIGHT; NONHUMAN; OPERON; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; REGULATORY MECHANISM; SPECTROSCOPY;

ACIDITHIOBACILLUS; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; IRON-SULFUR PROTEINS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; OPERON; SEQUENCE ALIGNMENT;

ACIDITHIOBACILLUS; ACIDITHIOBACILLUS FERROOXIDANS; ACIDITHIOBACILLUS FERROOXIDANS ATCC 23270; ESCHERICHIA COLI;

EID: 57849099250     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Agar, J. N., C. Krebs, J. Frazzon, B. H. Huynh, D. R. Dean, and M. K. Johnson. 2000. IscU as a scaffold for iron-sulfur cluster biosynthesis: Sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU. Biochemistry 39: 7856-7862.
2. Beinert, H., R. H. Holm, and E. Munck. 1997. Iron-sulfur clusters: Nature's modular, multipurpose structures. Science 277: 653-659.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
4. Bruscella, P., L. Cassagnaud, J. Ratouchniak, G. Brasseur, E. Lojou, R. Amils, and V. Bonnefoy. 2005. The HiPIP from the acidophilic Acidithiobacillus ferrooxidans is correctly processed and translocated in Escherichia coli, in spite of the periplasm pH difference between these two micro-organisms. Microbiology 151: 1421-1431.
5. Ding, H. and R. J. Clark. 2004. Characterization of iron binding in IscA, an ancient iron-sulphur cluster assembly protein. Biochem. J. 379: 433-440.
6. Ingledew, W. J. 1982. Thiobacillus ferrooxidans. The bioenergetics of an acidophilic chemoautotroph. Biochim. Biophys. Acta 683: 89-117.
7. Johnson, M. K. 1998. Iron-sulfur proteins: New roles for old clusters. Curr. Opin. Chem. Biol. 2: 173-181.
8. Kaut, A., H. Lange, K. Diekert, G. Kispal, and R. Lill. 2000. Isa1p is a component of the mitochondrial machinery for maturation of cellular iron-sulfur proteins and requires conserved cysteine residues for function. J. Biol. Chem. 275: 15955-15961.
9. Laemmli, U. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
10. Lovenberg, W., B. B. Buchanan, and J. C. Rabinowitz. 1963. Studies on the chemical nature of clostridial ferredoxin. J. Biol. Chem. 238: 3899-3913.
11. Mansy, S. S., G. Wu, K. K. Surerus, and J. A. Cowan. 2002. Iron-sulfur cluster biosynthesis: Thermatoga maritima IscU is a structured iron-sulfur cluster assembly protein. J. Biol. Chem. 277: 21397-21404.
12. Martin, R. G. and J. L. Rosner. 2001. The AraC transcriptional activators. Curr. Opin. Microbiol. 4: 132-137.
13. Nishio, K. and M. Nakai. 2000. Transfer of iron-sulfur cluster from NifU to apoferredoxin. J. Biol. Chem. 275: 22615-22618.
14. Rawlings, D. E. 2001. The molecular genetics of Thiobacillus ferrooxidans and other mesophilic, acidophilic, chemolithotrophic, iron- or sulfur-oxidizing bacteria. Hydrometallurgy 59: 187-201.
15. Rees, D. C. and J. B. Howard. 2003. The interface between the biological and inorganic worlds: Iron-sulfur metalloclusters. Science 300: 929-931.
16. Schwartz, C. J., O. Djaman, J. A. Imlay, and P. J. Kiley. 2000. The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli. Proc. Natl. Acad. Sci. USA 97: 9009-9014.
17. Schwartz, C. J., J. L. Giel, T. Patschkowski, C. Luther, F. J. Ruzicka, H. Beinert, and P. J. Kiley. 2001. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc. Natl. Acad. Sci. USA 98: 14895-14900.
18. Siegel, L. M. 1965. A direct microdetermination for sulfide. Anal. Biochem. 11: 126-132.
19. Sodeoka, M., C. Larson, L. Chen, W. Land, and G. Verdine. 1993. A multifunctional plasmid for protein expression by ECPCR: Overproduction of the p50 subunit of NF-KB. Bioorg. Med. Chem. Lett. 3: 1095-1100.
20. Takahashi, Y. and M. Nakamura. 1999. Functional assignment of the ORF2-iscSiscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli. J. Biochem. (Tokyo) 126: 917-926.
21. Tokumoto, U. and Y. Takahashi. 2001. Genetic analysis of the isc operon in Escherichia coli involved in the biogenesis of cellular iron-sulfur proteins. J. Biochem. (Tokyo) 130: 63-71.
22. Wollenberg, M., C. Berndt, E. Bill, J. D. Schwenn, and A. Seidler. 2003. A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe-2S] cluster between two protomers and transfers it to [2Fe-2S] and [4Fe-4S] apo proteins. Eur. J. Biochem. 270: 1662-1671.
23. Xia, B., H. Cheng, V. Bandarian, G. H. Reed, and J. L. Markley. 1996. Human ferredoxin: Overproduction in Escherichia coli, reconstitution in vitro, and spectroscopic studies of iron-sulfur cluster ligand cysteine-to-serine mutants. Biochemistry 35: 9488-9495.
24. Yang, J., J. P. Bitoun, and H. Ding. 2006. Interplay of IscA and IscU in biogenesis of iron-sulfur clusters. J. Biol. Chem. 281: 27956-27963.
25. Zheng, L., R. H. White, V. L. Cash, and D. R. Dean. 1994. Mechanism for the desulfurization of L-cysteine catalyzed by the nifs gene product. Biochemistry 33: 4714-4720.
26. Zheng, L., V. L. Cash, D. H. Flint, and D. R. Dean. 1998. Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii. J. Biol. Chem. 273: 13264-13272.