A microfluidic dual capillary probe to collect messenger RNA from adherent cells and spheroids

Analytical Biochemistry

Volumn 385, Issue 1, 2009, Pages 138-142

  Shiku, Hitoshi ^a   Yamakawa, Takeshi ^a   Nashimoto, Yuji ^a   Takahashi, Yasufumi ^a   Torisawa, Yu suke ^a   Yasukawa, Tomoyuki ^a   Ito Sasaki, Takahiro ^b   Yokoo, Masaki ^a   Abe, Hiroyuki ^b   Kambara, Hideki ^c,d   Matsue, Tomokazu ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b YAMAGATA UNIVERSITY   (Japan)

^c HITACHI LTD   (Japan)

^d TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

Microfluidics; Real time PCR; RT PCR; Single cell analysis

Indexed keywords

CYTOLOGY; LAMINAR FLOW; POLYMERASE CHAIN REACTION; PROBES; RNA; TRANSCRIPTION;

GENE EXPRESSION PROFILES; GLASS CAPILLARIES; INTEGRATED MICROFLUIDIC DEVICES; MESSENGER RNAS (MRNA); REAL-TIME PCR; REVERSE TRANSCRIPTION; RT-PCR; SINGLECELL ANALYSIS;

MICROFLUIDICS;

BETA1 INTEGRIN; BUFFER; MESSENGER RNA;

ADHERENT CELL; ARTICLE; CONTROLLED STUDY; CYTOLYSIS; HUMAN; HUMAN CELL; LAMINAR FLOW; MECHANICAL PROBE; MICROFLUIDIC DUAL CAPILLARY PROBE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; TARGET CELL; TUMOR SPHEROID;

EID: 57749195526     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.10.039     Document Type: Article

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