Expression and characterization of bioactive recombinant human α-lactalbumin in the milk of transgenic cloned cows

Journal of Dairy Science

Volumn 91, Issue 12, 2008, Pages 4466-4476

  Wang, J ^a   Yang, P ^a   Tang, B ^b   Sun, X ^a   Zhang, R ^a   Guo, C ^a   Gong, G ^a   Liu, Y ^b   Li, R ^b   Zhang, L ^b   Dai, Y ^a   Li, N ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b Beijing Genprotein Biotechnology Company   (China)

Author keywords

Human lactalbumin; Humanized milk; Protein glycosylation; Transgenic cow

Indexed keywords

BOS; BOVINAE;

EID: 57749115698     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2008-1189     Document Type: Article

References (38)

1. Barman, T. E. 1970. Purification and properties of bovine milk glyco-α-lactalbumin. Biochim. Biophys. Acta 214:242-244.
2. Bleck, G. T., B. R. White, D. J. Miller, and M. B. Wheeler. 1998. Production of bovine α-lactalbumin in the milk of transgenic pigs. J. Anim. Sci. 76:3072-3078.
3. Booij, L., W. Merens, C. R. Markus, and A. J. Van der Does. 2006. Diet rich in α-lactalbumin improves memory in unmedicated recovered depressed patients and matched controls. J. Psychopharmacol. 20:526-535.
4. Boston, W. S., G. T. Bleck, J. C. Conroy, M. B. Wheeler, and D. J. Miller. 2001. Short communication: Effects of increased expression of α-lactalbumin in transgenic mice on milk yield and pup growth. J. Dairy Sci. 84:620-622.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
6. 2 cells by enteropathogenic Escherichia coli, Salmonella Typhimurium and Shigella flexneri. FEMS Microbiol. Lett. 259:158-162.
7. Candiano, G., M. Bruschi, L. Musante, L. Santucci, G. M. Ghiggeri, B. Carnemolla, P. Orecchia, L. Zardi, and P. G. Righetti. 2004. Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25:1327-1333.
8. Eyestone, W. H. 1999. Production and breeding of transgenic cattle using in vitro embryo production technology. Theriogenology 51:509-517.
9. Fujiwara, Y., R. Takahashi, M. Hirabayashi, M. Ueda, T. Muramatsu, H. Yamanaka, and K. Sekikawa. 2003. Analysis of the flanking regions of the human α-lactalbumin gene responsible for position-effect independent expression. Gene 305:71-78.
10. Giuffrida, M. G., M. Cavaletto, C. Giunta, B. Neuteboom, A. Cantisani, L. Napolitano, V. Calderone, J. Godovac-Zimmermann, and A. Conti. 1997. The unusual amino acid triplet Asn-Ile-Cys is a glycosylation consensus site in human α-lactalbumin. J. Protein Chem. 16:747-753.
11. Gong, G., Y. Dai, B. Fan, H. Zhu, H. Wang, L. Wang, C. Fang, R. Wan, Y. Liu, R. Li, and N. Li. 2004. Production of transgenic blastocyst by nuclear transfer from different types of somatic cells in cattle. Sci. China C Life Sci. 47:183-189.
12. Gustafsson, L., I. Leijonhufvud, A. Aronsson, A. K. Mossberg, and C. Svanborg. 2004. Treatment of skin papillomas with topical α-lactalbumin- oleic acid. N. Engl. J. Med. 350:2663-2672.
13. Holpert, M., and T. G. Cooper. 1990. Improved assays of α-lactalbumin and galactosyltransferase. Anal. Biochem. 188:168-175.
14. Jackson, J. G., D. B. Janszen, B. Lönnerdal, E. L. Lien, K. P. Pramuk, and C. F. Kuhlman. 2004. A multinational study of α-lactalbumin concentrations in human milk. J. Nutr. Biochem. 15:517-521.
15. Laible, G., B. Brophy, D. Knighton, and D. N. Wells. 2007. Compositional analysis of dairy products derived from clones and cloned transgenic cattle. Theriogenology 67:166-177.
16. Layman, D. K. 2003. The role of leucine in weight loss diets and glucose homeostasis. J. Nutr. 133:261S-267S.
17. Lien, E. L. 2003. Infant formulas with increased concentrations of α-lactalbumin. Am. J. Clin. Nutr. 77:1555S-1558S.
18. Lönnerdal, B., and E. L. Lien. 2003. Nutritional and physiologic significance of α-lactalbumin in infants. Nutr. Rev. 61:295-305.
19. Molina, L. H., J. Kramm, C. Brito, B. Carrillo, M. Pinto, and A. Ferrando. 2006. Protein composition of milk from Holstein-Friesian dairy cows and its relationship with the genetic variants A and B of κ-casein and β-lactoglobulin (Part I). Int. J. Dairy Technol. 59:183-187.
20. Monaco, M. H., D. E. Gronlund, G. T. Bleck, W. L. Hurley, M. B. Wheeler, and S. M. Donovan. 2005. Mammary specific transgenic over-expression of insulin-like growth factor-I (IGF-I) increases pig milk IGF-I and IGF binding proteins, with no effect on milk composition or yield. Transgenic Res. 14:761-773.
21. Noble, M. S., S. Rodriguez-Zas, J. B. Cook, G. T. Bleck, W. L. Hurley, and M. B. Wheeler. 2002. Lactational performance of first-parity transgenic gilts expressing bovine α-lactalbumin in their milk. J. Anim. Sci. 80:1090-1096.
22. Permyakov, E. A., and L. J. Berliner. 2000. α-Lactalbumin: Structure and function. FEBS Lett. 473:269-274.
23. Permyakov, S. E., V. N. Uversky, D. B. Veprintsev, A. M. Cherskaya, C. L. Brooks, E. A. Permyakov, and L. J. Berliner. 2001. Mutating aspartate in the calcium-binding site of α-lactalbumin: Effects on the protein stability and cation binding. Protein Eng. 14:785-789.
24. Polejaeva, I. A., and K. H. Campbell. 2000. New advances in somatic cell nuclear transfer: Application in transgenesis. Theriogenology 53:117-126.
25. Prasad, R., B. G. Hudson, R. Butkowski, J. W. Hamilton, and K. E. Ebner. 1979. Resolution of the charge forms and amino acid sequence and location of a tryptic glycopeptide in rat α-lactalbumin. J. Biol. Chem. 254:10607-10614.
26. Rao, K. R., and K. Brew. 1989. Calcium regulates folding and disulfide-bond formation in α-lactalbumin. Biochem. Biophys. Res. Commun. 163:1390-1396.
27. Redfield, C., B. A. Schulman, M. A. Milhollen, P. S. Kim, and C. M. Dobson. 1999. α-Lactalbumin forms a compact molten globule in the absence of disulfide bonds. Nat. Struct. Biol. 6:948-952.
28. Saito, A., M. Usui, Y. Song, H. Azakami, and A. Kato. 2002. Secretion of glycosylated α-lactalbumin in yeast Pichia pastoris. J. Biochem. 132:77-82.
29. Schmitt, J. A., B. L. Jorissen, L. Dye, C. R. Markus, N. E. Deutz, and W. J. Riedel. 2005. Memory function in women with premenstrual complaints and the effect of serotonergic stimulation by acute administration of an α-lactalbumin protein. J. Psychopharmacol. 19:375-384.
30. Svensson, M., A. Hakansson, A. K. Mossberg, S. Linse, and C. Svanborg. 2000. Conversion of α-lactalbumin to a protein inducing apoptosis. Proc. Natl. Acad. Sci. USA 97:4221-4226.
31. Thompson, M. P., D. P. Brower, R. Jenness, and C. E. Kotts. 1989. Phylogenetic variations in the calcium-dependent electrophoretic shift of α-lactalbumin. J. Dairy Sci. 72:3156-3165.
32. Tian, X. C., C. Kubota, K. Sakashita, Y. Izaike, R. Okano, N. Tabara, C. Curchoe, L. Jacob, Y. Zhang, S. Smith, C. Bormann, J. Xu, M. Sato, S. Andrew, and X. Yang. 2005. Meat and milk compositions of bovine clones. Proc. Natl. Acad. Sci. USA 102:6261-6266.
33. Tsiaras, A. M., G. G. Bargouli, G. Banos, and C. M. Boscos. 2005. Effect of κ-casein and β-lactoglobulin loci on milk production traits and reproductive performance of Holstein cows. J. Dairy Sci. 88:327-334.
34. Ushida, Y., Y. Shimokawa, T. Toida, H. Matsui, and M. Takase. 2007. Bovine α-lactalbumin stimulates mucus metabolism in gastric mucosa. J. Dairy Sci. 90:541-546.
35. Walstra, P., and R. Jenness. 1984. Dairy Chemistry and Physics. John Wiley and Sons Inc., New York, NY.
36. Yamaguchi, M., Y. Ito, and S. Takahashi. 2007. Fourteen-week feeding test of meat and milk derived from cloned cattle in the rat. Theriogenology 67:152-165.
37. Yang, X., X. C. Tian, C. Kubota, R. Page, J. Xu, J. Cibelli, and G. Seidel Jr. 2007. Risk assessment of meat and milk from cloned animals. Nat. Biotechnol. 25:77-83.
38. Yu, S., G. Cao, B. Fan, N. Li, Y. Duan, Y. Dai, and L. Wang. 2004. The cloning of human α-lactalbumin gene and high-level expression in milk of transgenic mice. Prog. Biochem. Biophys. 31:244-248.