Resolving sub-synaptic compartments with double immunofluorescence labeling in hippocampal neurons

Journal of Neuroscience Methods

Volumn 176, Issue 2, 2009, Pages 78-84

  Di Biase, Valentina ^a   Flucher, Bernhard E ^a   Obermair, Gerald J ^a  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

Author keywords

Immunocytochemistry; Microscopy; Piccolo; Presynaptic; PSD 95; RIM; Subcellular localization; Synapsin; vGLUT1

Indexed keywords

ANIMAL CELL; ARTICLE; BRAIN NERVE CELL; CONTROLLED STUDY; HIPPOCAMPUS; IMMUNOFLUORESCENCE TEST; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SYNAPSE;

ANIMALS; CELLS, CULTURED; EMBRYO, MAMMALIAN; FLUORESCENT ANTIBODY TECHNIQUE; HIPPOCAMPUS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; MICE, INBRED BALB C; MICROSCOPY, FLUORESCENCE; NERVE TISSUE PROTEINS; NEURONS; PRESYNAPTIC TERMINALS; SYNAPSES;

EID: 57649240524     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2008.08.025     Document Type: Article

References (24)

1. Bellocchio E.E., Hu H., Pohorille A., Chan J., Pickel V.M., and Edwards R.H. The localization of the brain-specific inorganic phosphate transporter suggests a specific presynaptic role in glutamatergic transmission. J Neurosci 18 (1998) 8648-8659
2. Bolte S., and Cordelières F.P. A guided tour into subcellular colocalization analysis in light microscopy. J Microsc 224 (2006) 213-232
3. Boutté Y., Crosnier M.T., Carraro N., Traas J., and Satiat-Jeunemaitre B. The plasma membrane recycling pathway and cell polarity in plants: studies on PIN proteins. J Cell Sci 119 (2006) 1255-1265
4. Cases-Langhoff C., Voss B., Garner A.M., Appeltauer U., Takei K., Kindler S., et al. A novel 420 kDa protein associated with the presynaptic cytomatrix. Eur J Cell Biol 69 (1996) 214-223
5. Cho K.O., Hunt C.A., and Kennedy M.B. The rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein. Neuron 9 (1992) 929-942
6. Costes S.V., Daelemans D., Cho E.H., Dobbin Z., Pavlakis G., and Lockett S. Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophys J 86 (2004) 3993-4003
7. Fenster S.D., Chung W.J., Zhai R., Cases-Langhoff C., Voss B., Garner A.M., et al. A presynaptic zinc finger protein structurally related to bassoon. Neuron 25 (2000) 203-214
8. Fletcher T.L., De Camilli P., and Banker G. Synaptogenesis in hippocampal cultures: evidence indicating that axons and dendrites become competent to form synapses at different stages of neuronal development. J Neurosci 14 (1994) 6695-6706
9. Glynn M.W., and McAllister A.K. Immunocytochemistry and quantification of protein colocalization in cultured neurons. Nat Protoc 1 (2006) 1287-1296
10. Goslin K., Asmussen H., and Banker G. Rat hippocampal neurons in low-density culture. In: Banker G., and Goslin K. (Eds). Culturing nerve cells. second ed. (1998), The MIT Press, Cambridge 339-370
11. Jaskolski F., Mulle C., and Manzoni O.J. An automated method to quantify and visualize colocalized fluorescent signals. J Neurosci Methods 146 (2005) 42-49
12. Kozubek M., and Matula P. An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy. J Microsc 200 (2000) 206-217
13. Lachmanovich E., Shvartsman D.E., Malka Y., Botvin C., Henis Y.I., and Weiss A.M. Co-localization analysis of complex formation among membrane proteins by computerized fluorescence microscopy: application to immunofluorescence co-patching studies. J Microsc 212 (2003) 122-131
14. Lanni F., and Keller H.E. Microscopy and microscope optical systems. In: Yuste R., Lanni F., and Konnerth A. (Eds). Imaging neurons (2000), Cold Spring Harbor Laboratory Press, New York 1.1-1.72
15. o, and N-type calcium channel complex at a presynaptic nerve terminal: analysis by quantitative immunocolocalization. J Neurosci 24 (2004) 4070-4081
16. Manders E.M.M. Chromatic shift in multicolor confocal microscopy. J Microsc 185 (1997) 321-328
17. North A.J. Seeing is believing? A beginners' guide to practical pitfalls in image acquisition. J Cell Biol 172 (2006) 9-18
18. + channel SK3 is localized in nerve terminals of excitatory synapses of cultured mouse hippocampal neurons. Eur J Neurosci 17 (2003) 721-731
19. V1.2) to synaptic and extrasynaptic compartments in hippocampal neurons. Eur J Neurosci 19 (2004) 2109-2122
20. Sassoè-Pognetto M., Panzanelli P., Sieghart W., and Fritschy J.M. Colocalization of multiple GABA(A) receptor subtypes with gephyrin at postsynaptic sites. J Comp Neurol 420 (2000) 481-498
21. Siksou L., Rostaing P., Lechaire J.P., Boudier T., Ohtsuka T., Fejtova A., et al. Three-dimensional architecture of presynaptic terminal cytomatrix. J Neurosci 27 (2007) 6868-6877
22. Sun L., Bittner M.A., Holz R.W., and Rim. A component of the presynaptic active zone and modulator of exocytosis, binds 14-3-3 through its N terminus. J Biol Chem 278 (2003) 38301-38309
23. van Steensel B., van Binnendijk E.P., Hornsby C.D., van der Voort H.T., Krozowski Z.S., de Kloet E.R., et al. Partial colocalization of glucocorticoid and mineralocorticoid receptors in discrete compartments in nuclei of rat hippocampus neurons. J Cell Sci 109 (1996) 787-792
24. Wieser S., Moertelmaier M., Fuertbauer E., Stockinger H., and Schütz G.J. (Un)confined diffusion of CD59 in the plasma membrane determined by high-resolution single molecule microscopy. Biophys J 92 (2007) 3719-3728