Toll-like receptor 4 initiates an innate immune response to lipopolysaccharide in human conjunctival epithelial cells

Experimental Eye Research

Volumn 88, Issue 1, 2009, Pages 49-56

  Chung, So Hyang ^a   Kweon, Mi Na ^b   Lee, Hyung Keun ^c   Choi, Seung Il ^c   Yang, Jin Young ^b   Kim, Eung Kweon ^c,d  

^a The Catholic University of Korea   (South Korea)

^b International Vaccine Institute   (South Korea)

^c Yonsei University College of Medicine   (South Korea)

^d Yonsei University   (South Korea)

Author keywords

conjunctival epithelial cells; IL 6; IL 8; innate immune response; toll like receptor 4

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 6; INTERLEUKIN 8; TOLL LIKE RECEPTOR 4; TUMOR NECROSIS FACTOR RECEPTOR ASSOCIATED FACTOR 6;

ARTICLE; CELL SURFACE; CELLULAR DISTRIBUTION; CONJUNCTIVA EPITHELIUM; CONTROLLED STUDY; CYTOKINE PRODUCTION; EPITHELIUM CELL; HUMAN; HUMAN CELL; INNATE IMMUNITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; RECEPTOR UPREGULATION;

ANTIGENS, CD14; CELLS, CULTURED; CONJUNCTIVA; CYTOKINES; DOSE-RESPONSE RELATIONSHIP, IMMUNOLOGIC; EPITHELIAL CELLS; EYE PROTEINS; GENE EXPRESSION; HUMANS; IMMUNITY, INNATE; IMMUNITY, MUCOSAL; INFLAMMATION; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; LIPOPOLYSACCHARIDES; NEOPLASM PROTEINS; NF-KAPPA B; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TOLL-LIKE RECEPTOR 4;

EID: 57549118530     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exer.2008.09.017     Document Type: Article

References (39)

1. Abe T., Nakajima A., Matsunaga M., Sakuragi S., and Komatsu M. Decreased tear lactoferrin concentration in patients with chronic hepatitis C. Br. J. Ophthalmol. 83 (1999) 684-687
2. Abreu M.T., Vora P., Faure E., Thomas L.S., Amold E.T., and Arditi M. Decreased expression of toll-like receptor-4 and MD-2 correlates with intestinal epithelial cell protection against dysregulated proinflammatory gene expression in response to bacterial lipopolysaccharide. J. Immunol. 167 (2001) 1609-1616
3. Aderem A., and Ulevitch R.J. Toll-like receptors in the induction of the innate immune response. Nature 406 (2000) 782-787
4. Aristoteli L.P., Bojarski B., and Wilcox M.D. Isolation of conjunctival mucin and differential interaction with Pseudomonas aeruginosa strains of varied pathogenic potential. Exp. Eye Res. 77 (2003) 699-710
5. Aswad M.I., John T., Barza M., Kenyon K., and Baum J. Bacterial adherence to extended wear soft contact lenses. Ophthalmology 97 (1990) 296-302
6. Backhed F., Soderhall M., Ekmanm P., Normark S., and Richter-Dahlfors A. Induction of innate immune responses by Escherichia coli and purified lipopolysaccharide correlate with organ- and cell-specific expression of toll-like receptors within the human urinary tract. Cell. Microbiol. 3 (2001) 153-158
7. Becker M.N., Diamond G., Verghese M.W., and Randell S.H. CD14-dependent lipopolysaccharide-induced β-defensin-2 expression in human tracheobronchial epithelium. J. Biol. Chem. 275 (2000) 29731-29736
8. Berry M., Harris A., Lumb R., and Powell K. Commensal ocular bacteria degrade mucins. Br. J. Ophthalmol. 86 (2002) 1412-1416
9. Blais D.R., Vascotto S.G., Griffith M., and Altosaar I. LBP and CD14 secreted in tears by the lacrimal glands modulate the LPS response of corneal epithelial cells. Investig. Ophthalmol. Vis. Sci. 46 (2005) 4235-4244
10. Bonini S., Micera A., Iovieno A., Lambiase A., and Bonini S. Expression of toll-like receptors in healthy and allergic conjunctiva. Ophthalmology 112 (2005) 1528-1534
11. Cario E., Brown D., McKee M., Lynch-Devaney K., Gerken G., and Podolsky D.K. Commensal-associated molecular patterns induce selective toll-like receptor-trafficking from apical membrane to cytoplasmic compartments in polarized intestinal epithelium. Am. J. Pathol. 160 (2002) 165-173
12. Chow J.C., Young D.W., Golenbock D.T., Christ W.J., and Gusovsky F. Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction. J. Biol. Chem. 274 (1999) 10689-10692
13. Chung S.H., Lee J.H., Yoon J.H., Lee H.K., and Seo K.Y. Multi-layered culture of primary human conjunctival epithelial cells producing MUC5AC. Exp. Eye Res. 85 (2007) 226-233
14. Doyle A., Beigi B., Early A., Blake A., Eustace P., and Hone R. Adherence of bacteria to intraocular lenses: a prospective study. Br. J. Ophthalmol. 79 (1995) 347-349
15. Doyle S.E., O'Connell R., Vaidya S.A., Chow E.K., Yee K., and Cheng G. Toll-like receptor 3 mediates a more potent antiviral response than toll-like receptor 4. J. Immunol. 170 (2003) 3565-3571
16. Gioannini T.L., Teghanemt A., Zhang D., Coussens N.P., Dockstader W., Ramaswamy S., and Weiss J.P. Isolation of an endotoxin-MD-2 complex that produces toll-like receptor 4-dependent cell activation at picomolar concentrations. Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 4186-4191
17. Guha M., and Mackman N. LPS induction of gene expression in human monocytes. Cell. Signal. 13 (2001) 85-94
18. Guillot L., Medjane S., Le-Barillec K., Balloy V., Danel C., Chignard M., and Si-Tahar M. Response of human pulmonary epithelial cells to lipopolysaccharide involves toll-like receptor 4 (TLR4)-dependent signaling pathways: evidence for an intracellular compartmentalization of TLR4. J. Biol. Chem. 279 (2004) 2712-2718
19. Haynes R.J., Tighe P.J., and Dua H.S. Antimicrobial defensin peptides of the human ocular surface. Br. J. Ophthalmol. 83 (1999) 737-741
20. Hoshino K., Takeuchi O., Kawai T., Sanjo H., Ogawa T., Takeda Y., Takeda K., and Akira S. Cutting edge: toll-like receptor-4 deficient mice are hyporesponsive to lipopolysaccharide. Evidence for TLR4 as the lps gene product. J. Immunol. 162 (1999) 3749-3752
21. Kawai T., and Akira S. TLR signaling. Semin. Immunol. 19 (2007) 24-32
22. Krisanaprakornkit S., Kimball J.R., Weinberg A., Darveau R.P., Bainbridge B.W., and Dale B.A. Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier. Infect. Immun. 68 (2000) 2907-2915
23. Li J., Shen J., and Beuerman R.W. Expression of toll-like receptors in human limbal and conjunctival epithelial cells. Mol. Vis. 13 (2007) 813-822
24. -ΔΔCT method. Methods 25 (2001) 402-408
25. Medzhitov R., and Janeway C. Innate immune recognition: mechanisms and pathways. Immunol. Rev. 173 (2000) 89-97
26. Miyake K. Endotoxin recognition molecules, toll-like receptor 4-MD-2. Semin. Immunol. 16 (2004) 11-16
27. Nagai Y., Akashi S., Nagafuku M., Ogata H., Iwakura S., Akira S., Kitamura T., Kosugi A., Kimoto M., and Miyake K. Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat. Immunol. 3 (2002) 667-672
28. Pugin J., Schurer-Maly C.C., Leturcq D., Moriarty A., Ulevitch R.J., and Tobias P.S. Lipopolysaccharide activation of human endothelial and epithelial cells is mediated by lipopolysaccharide-binding protein and soluble CD14. Proc. Natl. Acad. Sci. U.S.A. 90 (1993) 2744-2748
29. Schilling J.D., Mulvey M.A., Vincent C.D., Lorenz R.G., and Hultgren S.J. Bacterial invasion augments epithelial cytokine responses to Escherichia coli through a lipopolysaccharide-dependent mechanism. J. Immunol. 166 (2001) 1148-1155
30. Shimazu R., Akashi S., Ogata H., Nagai Y., Fukudome K., Miyake K., and Kimoto M. MD-2, a molecule that confers lipopolysaccharide responsiveness on toll-like receptor 4. J. Exp. Med. 189 (1999) 1777-1782
31. Song P.I., Abraham T.A., Park Y., Zivony A.S., Harten B., Edelhauser H.F., Ward S.L., Armstrong C.A., and Ansel J.G. The expression of functional LPS receptor proteins CD14 and toll-like receptor 4 in human corneal epithelial cells. Investig. Ophthalmol. Vis. Sci. 42 (2001) 2867-2877
32. Takeda K., Kaisho T., and Akira S. Toll-like receptors. Annu. Rev. Immunol. 21 (2003) 335-376
33. Takeuchi O., Hoshino K., Kawai T., Sanjo H., Takeda H., Ogawa T., Takeda K., and Akira S. Differential roles of TLR2 and TLR4 in recognition of Gram-negative and Gram-positive bacterial cell wall components. Immunity 11 (1999) 443-451
34. Talreja J., Dileepan K., Puri S., Kabir M.H., and Segal D.M. Human conjunctival epithelial cells lack lipopolysaccharide responsiveness due to deficient expression of MD2 but respond after interferon-γ priming or soluble MD2 supplementation. Inflammation 29 (2005) 170-181
35. Tobias P.S., Soldau K., Gegner J.A., Mintz D., and Ulevitch R.J. Lipopolysaccharide binding protein-mediated complexation of lipopolysaccharide with soluble CD14. J. Biol. Chem. 270 (1995) 10482-10488
36. Uehara A., Sugawara S., and Takada H. Priming of human oral epithelial cells by interferon-γ to secrete cytokines in response to lipopolysaccharides, lipoteichoic acids and peptidoglycans. J. Med. Microbiol. 51 (2002) 626-634
37. Ueta M., Nochi T., Jang M.H., Park E.J., Igarashi O., Hino A., Kawasaki S., Shikina T., Hiroi T., Kinoshita S., and Kiyono H. Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium. J. Immunol. 173 (2004) 3337-3347
38. Ueta M., Hamuro J., Kiyono H., and Kinoshita S. Triggering of TLR3 by polyI:C in human corneal epithelial cells to induce inflammatory cytokines. Biochem. Biophys. Res. Commun. 331 (2005) 285-294
39. Wolfs T.G., Buurman W.A., van Schadewijk A., de Vries B., Daemen M.A., Hiemstra P.S., and van't Veer C. In vivo expression of toll-like receptor 2 and 4 by renal epithelial cells: IFN-γ and TNF-α mediated up-regulation during inflammation. J. Immunol. 168 (2002) 1286-1293