Development of a nested polymerase chain reaction assay for detection of Colletotrichum acutatum on symptomless strawberry leaves

Plant Disease

Volumn 92, Issue 12, 2008, Pages 1655-1661

  Perez Hernandez O ^a   Nam, M H ^b   Gleason, M L ^a   Kim, H G ^c  

^a IOWA STATE UNIVERSITY   (United States)

^b Nonsan Strawberry Experiment Station   (South Korea)

^c Chungnam National University   (South Korea)

Author keywords

Anthracnose; DNA extraction

Indexed keywords

ANTHRACNOSE; COMPOSITE SAMPLES; DNA EXTRACTION; NESTED PCR; NESTED POLYMERASE CHAIN REACTIONS; PLASTIC BAGS; POWERFUL TOOLS;

COMPUTER NETWORKS; COSMIC RAYS; DNA; GENES; NUCLEIC ACIDS; ORGANIC ACIDS; PLASTIC CONTAINERS; POLYMERIZATION; POLYMERS; PUMPS;

ASSAYS;

COLLETOTRICHUM; FRAGARIA X ANANASSA; GLOMERELLA ACUTATA;

EID: 57549112250     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS-92-12-1655     Document Type: Article

References (37)

1. Biggerstaff, C. M., Gleason, M. L., and Braun, E. J. 2000. Refinement of a nondestructive tomato seed assay for Clavibacter michiganensis subsp. michiganensis using used fiber. Seed Sci. Technol. 28:261-269.
2. Cook, R. T. 1993. Strawberry black spot caused by Colletotrichum acutatum. Pages 301-304 in: British Crop Protection Council Monograph No. 54: Plant Health and European Single Market. D. Ebbels, ed. Hampshire, UK.
3. Cullen, D. W., Lee, A. K., Toth. I. K., and Duncan, J. M. 2002. Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR. Plant Pathol. 51:281-292.
4. Dhingra, O. D., and Sinclair, J. B. 1985. Basic Plant Pathology Methods. CRC Press, Boca Raton, FL.
5. Doyle, J. J., and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13.
6. Freeman, S., Horowiz, S., and Sharon, A. 2001. Pathogenic and nonpathogenic lifestyles in Colletotrichum acutatum from strawberry and other plants. Phytopathology 91:986-992.
7. Freeman, S., and Katan, T. 1997. Identification of Colletotrichum species responsible for anthracnose and root necrosis of strawberry in Israel. Phytopathology 87:516-521.
8. Glen, M., Smith, A. H., Langrell, S. R. H., and Mohammed, C. L. 2007. Development of nested polymerase chain reaction detection of Mycosphaerella spp. and its application to the study of leaf disease in Eucalyptus plantations. Phytopathology 97:132-144.
9. Grote, D., Olmos, A., Kofoet, A., Tuset, J. J., Bertolini, E., and Cambra, M. 2002. Specific and sensitive detection of Phytophthora nicotianae by simple and nested-PCR. Eur. J. Plant Pathol. 108:197-207.
10. Heller, L. C., Davis, C. R., Peak, K. K., Wingfield, D., Cannons, A. C., Amuso, P. T., and Cattrani, J. 2003. Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR. Appl. Environ. Microbiol. 69:1844-1846.
11. Howard, C. M., Maas, J. L., Chandler, C. K., and Albregts, E. E. 1992. Anthracnose of strawberry caused by the Colletotrichum complex in Florida. Plant Dis. 76:976-981.
12. Ippolito, A., Schena, L., and Nigro, F. 2002. Detection of Phytophthora nicotianae and P. citrophthora in citrus roots and soils by nested PCR. Eur. J. Plant Pathol. 108:855-868.
13. Ito, S., Ushijima, Y., Fujii, Y., Tanaka, S., Kameya-Iwaki, M., Yashiwaru, S., and Kishi, F. 1998. Detection of viable cells of Ralstonia solanacearum in soil using a semi-selective medium and a PCR technique. J. Phytopathol. 146:369-384.
14. Jang, C. S., Lee, J. J., Kim, S. I., Song, J. Y., Yoo, S. J., and Kim, H. G. 2005. Specific detection of root rot pathogen, Cylindrocarpon destructans, using nested PCR from ginseng seedling. Res. Plant Dis. 11:48-55.
15. Leandro, L. F. S., Gleason, M. L., Nutter, F. W., Jr., Wegulo, S. N., and Dixon, P. M. 2001. Germination and sporulation of Colletotrichum acutatum on symptomless strawberry leaves. Phytopathology 91:659-664.
16. Legard, D. E. 2000. Colletotricum disease of strawberry in Florida. Pages 292-299 in: Colletotrichum: Host Specificity, Pathology, and Host-Pathogen Interaction. D. Prusky, S. Freeman, and M. B. Dickman, eds. American Phytopathological Society Press, St. Paul, MN.
17. Ma, Z., and Michailides, T. J. 2007. Approaches for eliminating PCR inhibitors and designing PCR primers for the detection of phytopathogenic fungi. Crop Prot. 26:145-161.
18. McCartney, H. A., Foster, S. J., Fraaije, B. A., and Ward, E. 2003. Molecular diagnostics for fungal plant pathogens. Pest Manage. Sci. 59:129-142.
19. Mertely, J. C., and Legard, D. E. 2004. Detection, isolation, and pathogenicity of Colletotrichum spp. from strawberry petioles. Plant Dis. 88:407-412.
20. Nutter, F. W., Jr., Esker, P. D., and Coelho Netto, R. A. 2006. Disease assessment concepts and the advancements made in improving the accuracy and precision of plant disease data. Eur. J. Plant Pathol. 115:95-103.
21. Parikka, P., and Lemmetty, A. 2004. Tracing latent infection of Colletotrichum acutatum on strawberry by PCR. Eur. J. Plant Pathol. 110:393-398.
22. Sambrook, J., and Russell, D. W. 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Schaad, N. W., Berthier-Schaad, Y., and Knorr, D. 2007. A high throughput membrane BIOPCR technique for ultra-sensitive detection of Pseudomonas syringae pv. phaseolicola. Plant Pathol. 56:1-8.
24. Smith, B. J. 1998. Anthracnose fruit rot (black spot). Pages 31-33 in: Compendium of Strawberry Diseases, 2nd ed. J. L. Maas, ed. The American Phytopathological Society, St. Paul, MN.
25. Sreenivasaprasad, S., Sharada, K., Brown, A. E., and Mills, P. R. 1996. PCR-based detection of Colletotrichum acutatum on strawberry. Plant Pathol. 45:650-655.
26. Sreenivasaprasad, S., and Talhinhas, P. 2005. Genotypic and phenotypic diversity in Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Mol. Plant Pathol. 6:361-378.
27. Talhinhas, P., Sreenivasaprasad, S., Neves-Martins, J., and Oliveira, H. 2002. Genetic and molecular characterization of Colletotrichum acutatum causing anthracnose of lupins. Phytopathology 92:986-996.
28. Thorpe, D. J., Harrington, T. C., and Uchida, J. Y. 2005. Pathogenicity, internal transcribed spacer-rDNA variation, and human dispersal of Ceratocystis fimbriata on the family Araceae. Phytopathology 95:316-323.
29. Tooley, P. W., Bunyard, B. A., Carras, M. M., and Hatziloukas, E. 1997. Development of PCR primers from internal transcribed spacer region 2 for detection of Phytophthora species infecting potatoes. Appl. Environ. Microbiol. 63:1467-1475.
30. Tu, J. C. 1985. An improved Mathur's medium for growth, sporulation and germination of spores of Colletotrichum lindemuthianum. Microbios 44:87-93.
31. Verhoeff, K. 1974. Latent infections by fungi. Annu. Rev. Phytopathol. 12:99-110.
32. Wallenhammar, A. C., and Arwidsson, O. 2001. Detection of Plasmodiophora brassicae by PCR in naturally infested soils. Eur. J. Plant Pathol. 107:313-321.
33. White, T. J., Bruns, T., Lee, S., and Tayler, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pages 315-322 in: PCR Protocols: A Guide to Methods and Applications. D. H. Gelfland, M. A. Innis, J. J. Sninsky, and T. J. White, eds. Academic Press, San Diego, CA.
34. Wilson, I. G. 1997. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 63:3741-3751.
35. Wilson, M., and Lindow, S. E. 1992. Relationship of total viable and culturable cells in epiphytic populations of Pseudomonas syringae. Appl. Environ. Microbiol. 58:3908-3913.
36. Wise, K. A., Leandro, L. F. S., Wegulo, S. N., and Gleason, M. L. 2003. Survival and dispersal of Colletotrichum acutatum on strawberry foliage under field conditions. Adv. Strawberry Res. 22:14-19.
37. Yang, X., Madden, L. V., Reichard, D. L., Wilson, L. L., and Ellis, M. A. 1992. Splash dispersal of Colletotrichum acutatum and Phytophthora cactorum from strawberry fruit by single drop impactions. Phytopathology 82:332-340.