Rapid detection of porcine reproductive and respiratory syndrome virus by reverse transcription loop-mediated isothermal amplification assay

Journal of Virological Methods

Volumn 155, Issue 1, 2009, Pages 55-60

  Li, Qiang ^a   Zhou, Qing feng ^b   Xue, Chun yi ^a   Ma, Jing yun ^c   Zhu, Dao zhong ^c   Cao, Yong chang ^a  

^a SUN YAT SEN UNIVERSITY   (China)

^b Guangdong Wen's Foodstuffs Group Co Ltd   (China)

^c SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

PRRSV; RT LAMP; RT PCR

Indexed keywords

RESTRICTION ENDONUCLEASE;

AGAR GEL ELECTROPHORESIS; ARTERIVIRUS; ARTICLE; ASSAY; COLORIMETRY; CROSS REACTION; DIAGNOSTIC PROCEDURE; GENE AMPLIFICATION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS DIAGNOSIS; VIRUS EXAMINATION; VIRUS ISOLATION;

ANIMALS; CELL LINE; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SWINE; TIME FACTORS;

PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SIMIAN IMMUNODEFICIENCY VIRUS; SUIDAE;

EID: 57549099248     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.09.012     Document Type: Article

References (25)

1. Cavanagh D. Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Arch. Virol. 142 (1997) 629-633
2. Christopher-Hennings J., Nelson E.A., Nelson J.K., Hines R.J., Swenson S.L., Hill H.T., Zimmerman J.J., Katz J.B., Yaeger M.J., Chase C.C., and Benfield D.A. Detection of porcine reproductive and respiratory syndrome virus in boar semen by PCR. J. Clin. Microbiol. 33 (1995) 1730-1734
3. Chung W.B., Chan W.H., Chaung H.C., Lien Y., Wu C.C., and Huang Y.L. Real-time PCR for quantitation of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in naturally-infected and challenged pigs. J. Virol. Methods 124 (2005) 11-19
4. Conzelmann K.K., Visser N., Van Woensel P., and Thiel H.J. Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group. Virology 193 (1993) 329-339
5. Elazhary Y., Weber J., Bikour H., Morin M., and Girard C. Mystery swine disease in Canada. Vet. Rec. 129 (1991) 495-496
6. Grebennikova T.V., Clouser D.F., Vorwald A.C., Musienko M.I., Mengeling W.L., Lager K.M., Wesley R.D., Biketov S.F., Zaberezhny A.D., Aliper T.I., and Nepoklonov E.A. Genomic characterization of virulent, attenuated, and revertant passages of a North American porcine reproductive and respiratory syndrome virus strain. Virology 321 (2004) 383-390
7. Halbur P.G., Andrews J.J., Huffman E.L., Paul P.S., Meng X.J., and Niyo Y. Development of a streptavidin-biotin immunoperoxidase procedure for the detection of porcine reproductive and respiratory syndrome virus antigen in porcine lung. J. Vet. Diagn. Invest. 6 (1994) 254-257
8. Halbur P.G., Paul P.S., Frey M.L., Landgraf J., Eernisse K., Meng X.J., Lum M.A., Andrews J.J., and Rathje J.A. Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. Vet. Pathol. 32 (1995) 648-660
9. Kim H.S., Kwang J., Yoon I.J., Joo H.S., and Frey M.L. Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line. Arch. Virol. 133 (1993) 477-483
10. Kono Y., Kanno T., Shimizu M., Yamada S., Ohashi S., Nakamine M., and Shirai J. Nested PCR for detection and typing of porcine reproductive and respiratory syndrome (PRRS) virus in pigs. J. Vet. Med. Sci. 58 (1996) 941-946
11. Larochelle R., and Magar R. Comparison of immunogold silver staining (IGSS) with two immunoperoxidase staining systems for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) antigens in formalin-fixed tissues. J. Vet. Diagn. Invest. 7 (1995) 540-543
12. Larochelle R., and Magar R. Differentiation of North American and European porcine reproductive and respiratory syndrome virus genotypes by in situ hybridization. J. Virol. Methods 68 (1997) 161-1688
13. Larochelle R., and Magar R. Evaluation of the presence of porcine reproductive and respiratory syndrome virus in packaged pig meat using virus isolation and polymerase chain reaction (PCR) method. Vet. Microbiol. 58 (1997) 1-8
14. Mardassi H., Wilson L., Mounir S., and Dea S. Detection of porcine reproductive and respiratory syndrome virus and efficient differentiation between Canadian and European strains by reverse transcription and PCR amplification. J. Clin. Microbiol. 32 (1994) 2197-2203
15. Mori Y., Nagamine K., Tomita N., and Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 289 (2001) 150-154
16. Nagamine K., Hase T., and Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell Probes 16 (2002) 223-229
17. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., and Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28 (2000) E63
18. Stadejek T., Stankevicius A., Storgaard T., Oleksiewicz M.B., Belak S., Drew T.W., and Pejsak Z. Identification of radically different variants of porcine reproductive and respiratory syndrome virus in Eastern Europe: towards a common ancestor for European and American viruses. J. Gen. Virol. 83 (2002) 1861-1873
19. Sur J.H., Cooper V.L., Galeota J.A., Hesse R.A., Doster A.R., and Osorio F.A. In vivo detection of porcine reproductive and respiratory syndrome virus RNA by in situ hybridization at different times postinfection. J. Clin. Microbiol. 34 (1996) 2280-2286
20. Tian K., Yu X., Zhao T., Feng Y., Cao Z., Wang C., Hu Y., Chen X., Hu D., Tian X., Liu D., Zhang S., Deng X., Ding Y., Yang L., Zhang Y., Xiao H., Qiao M., Wang B., Hou L., Wang X., Yang X., Kang L., Sun M., Jin P., Wang S., Kitamura Y., Yan J., and Gao G.F. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS ONE 2 (2007) e526
21. Van Woensel P., Van der Wouw J., and Visser N. Detection of porcine reproductive respiratory syndrome virus by the polymerase chain reaction. J. Virol. Methods 47 (1994) 273-278
22. Wensvoort G., Terpstra C., Pol J.M.A., Laak E.A., Bloemraad M., De Kluyver E.P., Kragten C., Van Buiten L., Den Besten A., Wagenaar F., Broekhuijsen J.M., Moonen P.L.J.M., Zetstra T., De Boer E.A., Tibben H.J., De Jong M.F., Van't Veld P., Groenland G.J.R., Van Gennep J.A., Voets M.T., Verheijden J.H.M., and Braamskamp J. Mystery swine disease in theNetherlands: the isolation of Lelystad virus. Vet. Quart. 13 (1991) 121-130
23. Wensvoort G. Lelystad virus and the porcine epidemic abortion and respiratory syndrome. Vet. Res. 24 (1993) 117-124
24. Wesley R.D., Mengeling W.L., Lager K.M., Clouser D.F., Landgraf J.G., and Frey M.L. Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5. J. Vet. Diagn. Invest. 10 (1998) 140-144
25. Yoon I.J., Joo H.S., Christianson W.T., Kim H.S., Collins J.E., Carlson J.H., and Dee S.A. Isolation of a cytopathic virus from weak pigs on farms with a history of swine infertility and respiratory syndrome. J. Vet. Diagn. Invest. 4 (1992) 139-143