Recombinant expression and characterization of an epididymis-specific antimicrobial peptide BIN1b/SPAG11E

Journal of Biotechnology

Volumn 139, Issue 1, 2009, Pages 33-37

  Guo, Chenyun ^a   Diao, Hua ^b,c   Lian, Yandong ^a   Yu, Heguo ^c   Gao, Hongchang ^a   Zhang, Yonglian ^b,c   Lin, Donghai ^a  

^a SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

^b INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^c SHANGHAI INSTITUTE OF PLANNED PARENTHOOD RESEARCH   (China)

Author keywords

Antimicrobial activity; BIN1b; CD; HSQC; Oligomerization; Size exclusion

Indexed keywords

ANTIMICROBIAL ACTIVITY; BIN1B; CD; HSQC; SIZE-EXCLUSION;

AMINES; COLLOIDS; DICHROISM; ESCHERICHIA COLI; GELATION; MONOMERS; OLIGOMERIZATION; OPTICAL PROPERTIES; POLYPEPTIDES; POROUS MATERIALS; SODIUM CHLORIDE; THREE DIMENSIONAL;

FILTRATION;

BETA DEFENSIN; HYBRID PROTEIN; POLYPEPTIDE ANTIBIOTIC AGENT; PROTEIN BIN1B; RECOMBINANT PROTEIN; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY; ARTICLE; CIRCULAR DICHROISM; EPIDIDYMIS; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; HETERONUCLEAR SINGLE QUANTUM COHERENCE; OLIGOMERIZATION; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

AMINO ACID SEQUENCE; ANTIMICROBIAL CATIONIC PEPTIDES; BETA-DEFENSINS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; COLONY COUNT, MICROBIAL; EPIDIDYMIS; ESCHERICHIA COLI; HUMANS; MALE; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS;

EID: 57549094803     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.10.003     Document Type: Article

References (11)

1. Cipáková I., Hostinová E., Gasperík J., and Velebný V. High-level expression and purification of a recombinant hBD-1 fused to LMM protein in Escherichia coli. Protein Expr. Purif. 37 (2004) 207-212
2. Diao H., Guo C.Y., Li D.H., and Zhang Y.L. Intein-mediated expression is an effective approach in the study of beta-defensins. Biochem. Biophys. Res. Commun. 357 (2007) 840-846
3. Gronenborn A.M., Filpula D.R., Essig N.Z., Achari A., Whitlow M., Wingfield P.T., and Clore G.M. A novel, highly stable fold of the immunoglobulin binding domain of streptococcal protein G. Science 253 (1991) 657-661
4. Li P., Chan H.C., He B., So S.C., Chung Y.W., Shang Q., Zhang Y.D., and Zhang Y.L. An antimicrobial peptide gene found in the male reproductive system of rats. Science 291 (2001) 1783-1785
5. Nagaoka I., Hirota S., Yomogida S., Ohwada A., and Hirata M. Synergistic actions of antibacterial neutrophil defensins and cathelicidins. Inflamm. Res. 49 (2000) 73-79
6. Peng L., Xu Z.N., Fang X.M., Wang F., and Cen P.L. High-level expression of soluble human beta-defensin-2 in Escherichia coli. Process Biochem. 39 (2004) 2199-2205
7. Sambrook J., and Russell D.W. Molecular Cloning: A Laboratory Manual. third ed. (2001), Cold Spring Harbor Laboratory Press, NY
8. Schlapschy M., Grimm S., and Skerra A. A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Eng. Des. Sel. 19 (2006) 385-390
9. Schröder J.M. Epithelial peptide antibiotics. Biochem. Pharmacol. 57 (1999) 121-134
10. Schutte B.C., Mitros J.P., Bartlett J.A., Walters J.D., Jia H.P., Welsh M.J., Casavant T.L., and McCray P.B. Discovery of five conserved beta-defensin gene clusters using a computational search strategy. Proc. Natl. Acad. Sci. 99 (2002) 2129-2133
11. Zhou C.X., Zhang Y.L., Xiao L., Zheng M., Leung K.M., Chan M.Y., Lo P.S., Tsang L.L., Wong H.Y., Ho L.S., Chung Y.W., and Chan H.C. An epididymis-specific β-defensin is important for the initiation of sperm maturation. Nat. Cell. Biol. 6 (2004) 458-464