Purification and gene cloning of α-methylserine aldolase from Ralstonia sp. strain AJ110405 and application of the enzyme in the synthesis of α-methyl-L-serine

Applied and Environmental Microbiology

Volumn 74, Issue 24, 2008, Pages 7596-7599

  Nozaki, Hiroyuki ^a   Kuroda, Shinji ^a   Watanabe, Kunihiko ^a   Yokozeki, Kenzo ^a  

^a AJINOMOTO CO INC   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CLONING; ENCODING (SYMBOLS); ENZYMES; ESCHERICHIA COLI; FORMALDEHYDE; GENETIC ENGINEERING; PURIFICATION; SUBSTRATES;

ALDOLASE; CELL CATALYSTS; E. COLI; ENZYMATIC ACTIVITIES; GENE CLONINGS; HIGH YIELDS; HOMODIMER; HOMOGENEOUS FORMS; INTERCONVERSION; RALSTONIA; SYNTHESIS OF; SYNTHESIS REACTIONS; TETRAHYDROFOLATE;

GENE ENCODING;

ALPHA METHYLSERINE ALDOLASE; BACTERIAL ENZYME; UNCLASSIFIED DRUG;

AMINO ACID; BACTERIUM; BIOCHEMISTRY; CATALYSIS; CHEMICAL COMPOSITION; ENZYME ACTIVITY; FORMALDEHYDE; GENE EXPRESSION;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL STRAIN; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; RALSTONIA;

ALANINE; AMARANTHACEAE; BACTERIAL PROTEINS; CLONING, MOLECULAR; COMAMONADACEAE; DIMERIZATION; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; FORMALDEHYDE; FRUCTOSE-BISPHOSPHATE ALDOLASE; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; PROTEIN SUBUNITS; RALSTONIA; RECOMBINANT PROTEINS; SERINE; SUBSTRATE SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI; RALSTONIA SP.; VARIOVORAX PARADOXUS;

EID: 57449121166     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00677-08     Document Type: Article

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