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NIPAm (1.4 g) and 0.03 g of cross-linker (BIS) were dissolved in 100 mL of water. The mixture was transferred into a 250 mL round-bottom flask and was heated at 70°C under a gentle nitrogen stream for 45 min. AAc (0.08 g in 10gm of water) was added to the above mixtures. Finally, the initiator, potassium perox-disulfate (KPS) (0.05 g dissolved in 10 g of water) was added to initiate the polymerization. The reaction mixture was kept at 70°C under nitrogen at least for 6 h to complete the reaction. Finally, the suspensions were purified by dialysed followed by centrifugation under high rate to remove the unreacted monomers, linear chains and ionic impurities.
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h,45 is the hydrodynamic radius at 45°C at pH = 3.2. It was taken into account that in the collapsed state, the microgel particle contains 50% water.
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