An in-house method for the detection and quantification of HCV in serum samples using a TaqMan assay real time PCR approach

Clinical Chemistry and Laboratory Medicine

Volumn 46, Issue 12, 2008, Pages 1729-1731

  Bashiardes, Stavros ^a   Richter, Jan ^a   Christodoulou, Christina G ^a  

^a CYPRUS INSTITUTE OF NEUROLOGY AND GENETICS   (Cyprus)

Author keywords

Hepatitis C; Hepatitis C virus (HCV); Quantification; Real time PCR

Indexed keywords

ARTICLE; GENOTYPE; HEPATITIS C VIRUS; PRIORITY JOURNAL; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS GENOME; VIRUS LOAD;

BASE SEQUENCE; HEPACIVIRUS; HEPATITIS C; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC;

HEPATITIS C VIRUS;

EID: 57449119294     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2008.343     Document Type: Article

References (7)

1. Craxì A, Laffi G, Zignego AL. Hepatitis C virus (HCV) infection: a systemic disease. Mol Aspects Med 2008;29:85-95.
2. Jauncey M, Micallef JM, Gilmour S, Amin J, White PA, Rawlinson W, et al. Clearance of hepatitis C virus after newly acquired infection in injection drug users. J Infect Dis 2004;190:1270-4.
3. Scott JD, Gretch DR. Molecular diagnostics of hepatitis C virus infection: a systematic review. J Am Med Assoc 2007;297:724-32.
4. Shuhart MC, Bronner MP, Gretch DR, Thomassen LV, Wartelle CF, Tateyama H, et al. Histological and clinical outcome after liver transplantation for hepatitis C. Hepatology 1997;26:1646-52.
5. Simmonds P, Holmes EC, Cha TA, Chan SW, McOmish F, Irvine B, et al. Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. J Gen Virol 1993;74:2391-9.
6. McHutchison JG, Gordon SC, Schiff ER, Shiffman ML, Lee WM, Rustgi VK, et al. Interferon α-2b alone or in combination with ribavirin as initial treatment for chronic hepatitis C. Hepatitis Interventional Therapy Group. N Engl J Med 1998;339:1485-92.
7. Sábato MF, Shiffman ML, Langley MR, Wilkinson DS, Ferreira-Gonzalez A. Comparison of performance characteristics of three real-time reverse transcription-PCR test systems for detection and quantification of hepatitis C virus. J Clin Microbiol 2007;45:2529-36.