Rapid multiplex PCR assay for simultaneous detection of major antibiotic resistance determinants in clinical isolates of bacteroides Fragilis

Journal of Rapid Methods and Automation in Microbiology

Volumn 16, Issue 4, 2008, Pages 381-393

  Pumbwe, Lilian ^a,b   Curzon, Matthew ^a   Wexler, Hannah M ^a,b  

^a VA GREATER LOS ANGELES HEALTHCARE SYSTEM   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); BACTEROIDES FRAGILIS;

EID: 57349194356     PISSN: 10603999     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1745-4581.2008.00143.x     Document Type: Article

References (28)

1. BACIC, M., PARKER, A.C., STAGG, J., WHITLEY, H.P., WELLS, W.G.F., JACOB, L.A. SMITH, C.J. 2005. Genetic and structural analysis of the Bacteroides conjugative transposon ctn341. J. Bacteriol. 187, 2858 2869.
2. CHAUDHRY, R., MATHUR, P., DHAWAN, D. KUMAR, L. 2001. Emergence of metronidazole-resistant Bacteroides fragilis. India. Emerg. Infect. 7, 485 486.
3. CHIU, C.H., SU, L.H., CHU, C.H., WANG, M.H., YEH, C.M., WEILL, F.X. CHU, C. 2006. Detection of multidrug-resistant Salmonella enterica Serovar Typhimurium Phage Types DT102, DT104, and U302 by multiplex PCR. J. Clin. Microbiol. 44, 2354 2358.
4. CHUNG, W.O., WERCKENTHIN, C., SCHWARZ, S. ROBERTS, M.C. 1999. Host range of the ermF rRNA methylase gene in bacteria of human and animal origin. J. Antimicrob. Chemother. 43, 5 14.
5. GAL, M. BRAZIER, J.S. 2004. Metronidazole resistance in Bacteroides spp. carrying nim genes and the selection of slow-growing metronidazole-resistant mutants. J. Antimicrob. Chemother. 54, 109 116.
6. GUPTA, A., VLAMAKIS, H., SHOEMAKER, N. SALYERS, A. 2003. A new Bacteroides conjugative transposon that carries an ermB gene. Appl. Environ. Microbiol. 69, 6455 6463.
7. GUTACKER, M., VALSANGIACOMO, C. PIFFARETTI, J.C. 2002. Identification of two genetic groups in Bacteroides fragilis by multilocus enzyme electrophoresis: Distribution of antibiotic resistance (cfiA, cepA) and enterotoxin (bft) encoding genes. Microbiology 146, 1241 1254.
8. HECHT, D.W. 2004. Prevalence of antibiotic resistance in anaerobic bacteria: Worrisome developments. Clin. Infect. Dis. 39, 92 97.
9. HERRERA-LEÓN, L., MOLINA, T., SAÍZ, P., SÁEZ-NIETO, J.A. JIMÉNEZ, M.S. 2005. New multiplex PCR for rapid detection of isoniazid-resistant Mycobacterium tuberculosis clinical isolates. Antimicrob. Agents Chemother. 49, 44 147.
10. LOFMARK, S., FANG, H., HEDBERG, M. EDLUND, C. 2005. Inducible metronidazole resistance and nim genes in clinical Bacteroides fragilis group isolates. Antimicrob. Agents Chemother. 49, 1253 1256.
11. NCCLS. 2007. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria, 7th Ed. Approved standard. CLSI (formerly NCCLS) publication no. M11-A7. 2007. Clinical Laboratory Standards Institute, Wayne, PA.
12. NGUYEN, M.H., YU, V.L., MORRIS, A.J., MCDERMOTT, L., WAGENER, M.W., HARRELL, L. SNYDMAN, D.R. 2000. Antimicrobial resistance and clinical outcome of Bacteroides bacteremia: Findings of a multicenter prospective observational trial. Clin. Infect. Dis. 30, 870 876.
13. OTEO, J., ARACIL, B., ALOS, J.I. GOMEZ-GARCES, J.L. 2000. High prevalence of resistance to clindamycin in Bacteroides fragilis group isolates. J. Antimicrob. Chemother. 45, 691 693.
14. PANKUCH, G.A., JACOBS, M.R. APPELBAUM, P.C. 1993. Susceptibilities of 428 gram-positive and -negative anaerobic bacteria to Bay y3118 compared with their susceptibilities to ciprofloxacin, clindamycin, metronidazole, piperacillin, piperacillin-tazobactam and cefoxitin. Antimicrob. Agents Chemother. 37, 1649 1654.
15. PUMBWE, L., WAREHAM, D.W., ADUSE-OPOKU, J., BRAZIER, J.H. WEXLER, H.M. 2007. Genetic analysis of mechanisms of multidrug resistance in a clinical isolate of Bacteroides fragilis. Clin. Microbiol. Infect. 13, 183 189.
16. RASMUSSEN, J.L., ODELSON, D.A. MACRINA, F.L. 1986. Complete nucleotide sequence and transcription of ermF, a macrolide-lincosamide-streptogramin B resistance determinant from Bacteroides fragilis. J. Bacteriol. 168, 523 533.
17. ROSENBLATT, J.E. BROOK, I. 1993. Clinical relevance of susceptibility testing of anaerobic bacteria. Clin. Infect. Dis. 16 (Suppl. 4 S446 S448.
18. SALONEN, J.H., EEROLA, E. MEURMAN, O. 1998. Clinical significance and outcome of anaerobic bacteremia. Clin. Infect. Dis. 26, 1413 1417.
19. SCHAPIRO, J.M., GUPTA, R., STEFANSSON, E., FANG, F.C. LIMAYE, A.P. 2004. Isolation of metronidazole-resistant Bacteroides fragilis carrying the nimA nitroreductase gene from a patient in Washington State. J. Clin. Microbiol. 42, 4127 4129.
20. SCHAUMANN, R., PETZOLD, S. RODLOFF, A.C. 2005. Inducible metronidazole resistance in nim-positive and nim-negative Bacteroides fragilis group strains after several passages metronidazole containing Columbia agar plates. Infection 33, 368 372.
21. SHOEMAKER, N.B., VLAMAKIS, H., HAYES, K. SALYERS, A.A. 2003. Evidence for extensive resistance gene transfer among Bacteroides spp. and among Bacteroides and other genera in the human colon. Appl. Environ. Microbiol. 67, 561 568.
22. SNYDMAN, D.R., JACOBUS, N.V., MCDERMOTT, L.A., RUTHAZER, R., GOLDSTEIN, E.J. FINEGOLD, S.M. 2002. National survey on the susceptibility of Bacteroides fragilis group: Report and analysis of trends for 1997-2000. Clin. Infect. Dis. 35, S126 S134.
23. SOKI, J., FODOR, E., HECHT, D.W., EDWARDS, R., ROTIMI, V.O. KEREKES, I. 2004. Molecular characterization of imipenem-resistant, cfiA-positive Bacteroides fragilis isolates from the USA, Hungary and Kuwait. J. Med. Microbiol. 53, 413 419.
24. STROMMENGER, B., KETTLITZ, C., WERNER, G. WITTE, W. 2003. Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. J. Clin. Microbiol. 41, 4089 4094.
25. TRINH, S. REYSSET, G. 1996. Detection by PCR of the nim genes encoding 5-nitroimidazole resistance in Bacteroides spp. J. Clin. Microbiol. 34, 2078 2084.
26. WANG, Y., WANG, G.R., SHELBY, A., SHOEMAKER, N.B. SALYERS, A.A. 2003. A newly discovered Bacteroides conjugative transposon, CTnGERM1, contains genes also found in gram-positive bacteria. Appl. Environ. Microbiol. 69, 4595 4603.
27. WEISBLUM, B. 1995. Erythromycin resistance by ribosome modification. Antimicrob. Agents Chemother. 39, 577 585.
28. WEXLER, H.M., MOLITORIS, D.E., MURRAY, P.R., WASHINGTON, J., ZABRANSKY, R.J., EDELSTEIN, P.H. FINEGOLD, S.M. 1996. Comparison of spiral gradient endpoint and agar dilution methods for susceptibility testing of anaerobic bacteria: A multilaboratory collaborative evaluation. J. Clin. Microbiol. 34, 170 174.