The Ussing chamber and measurement of drug actions on mucosal ion transport

Current Protocols in Pharmacology

Volumn , Issue SUPPL. 41, 2008, Pages

  Brown, David R ^a   O'Grady, Scott M ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Airways; Epithelial cell culture; Epithelium; Intestine; Pharmacokinetics; Skin; Transepithelial ion transport

Indexed keywords

BENZAMIL; OPIATE RECEPTOR;

BIOCHEMICAL EQUIPMENT; CELL SURFACE; CHLORIDE TRANSPORT; CONCENTRATION RESPONSE; DRUG DETERMINATION; DRUG MECHANISM; DRUG TRANSPORT; ELECTRIC POTENTIAL; ION TRANSPORT; MEMBRANE TRANSPORT; METHODOLOGY; MONOLAYER CULTURE; MOUTH MUCOSA; MUCOSA; MUCOSAL ION TRANSPORT; NONHUMAN; PRIORITY JOURNAL; REACTION TIME; REVIEW; SODIUM TRANSPORT; USSING CHAMBER SYSTEM; VOLTAGE CLAMP;

ANIMALS; CELLS, CULTURED; DIFFUSION CHAMBERS, CULTURE; ELECTROPHYSIOLOGY; EQUIPMENT DESIGN; INTESTINAL MUCOSA; ION PUMPS; ION TRANSPORT; MEMBRANE POTENTIALS; PHARMACOKINETICS; RADIOISOTOPES; RATS; SPECIMEN HANDLING; TISSUE SURVIVAL;

EID: 57349175576     PISSN: 19348282     EISSN: 19348290     Source Type: Journal    
DOI: 10.1002/0471141755.ph0712s41     Document Type: Review

References (21)

1. Bohets, H., Annaert, P., Mannens, G., Van Beijsterveldt, L., Anciaux, K., Verboven, P., Meuldermans, W., and Lavrijsen, K. 2001. Strategies for absorption screening in drug discovery and development. Curr. Top. Med. Chem. 1:367-383.
2. Brown, D.R., Parsons, A.M., and O'Grady, S.M. 1992. Substance P produces sodium and bicarbonate secretion in porcine jejunal mucosa through an action on enteric neurons. J. Pharmacol. Exp. Ther. 261:1206-1212.
3. 3- transport by carbachol in pig distal jejunum. 1991. Eur. J. Pharmacol. 193:257-264.
4. Chang, E.B., Field, M., and Miller, R.J. 1982. Alpha2-adrenergic receptor regulation of ion transport in rabbit ileum. Am. J. Physiol. 242:G237-G242.
5. 2. J. Physiol. (London) 508:31-47.
6. Dobbins, J., Racusen, L., and Binder, H.J. 1980. Effect of D-alanine methionine enkephalin amide on ion transport in rabbit ileum. J. Clin. Invest. 66:19-28.
7. Gotoh, Y., Kamada, N., and Momose, D. 2005. The advantages of the Ussing chamber in drug absorption. J. Biomol. Screen. 10:517-523.
8. Kachur, J.F., Miller, R.J., and Field, M. 1980. Control of guinea pig intestinal electrolyte secretion by a delta-opiate receptor. Proc. Natl. Acad. Sci. U.S.A. 77:2753-2756.
9. Kim, M.K. and Shim, C.K. 2006. The transport of organic cations in the small intestine: Current knowledge and emerging concepts. Arch. Pharm. Res. 29:605-616.
10. Kitchen, I. 1984. Textbook of in vitro Practical Pharmacology. Blackwell Sci. Publ., Oxford, U.K.
11. Koefoed-Johnsen, V. and Ussing, H.H. 1958. The nature of the frog skin potential. Acta Physiol. Scand. 42: 298-308.
12. Larsen, E.H. 2002. Hans H. Ussing - scientific work: Contemporary significance and perspectives. Biochim. Biophys. Acta 1566:2-15.
13. Lindemann, B. 2001. HansUssing, experiments and models. J. Membr. Biol. 184:203-210.
14. Quito, F.L. and Brown, D.R. 1991. Neurohormonal regulation of ion transport in the porcine distal jejunum. Enhancement of sodium and chloride absorption by submucosal opiate receptors. J. Pharmacol. Exp. Ther. 256:833-840.
15. Palmer, L. G., Edelman, I.S., and B. Lindemann, B. 1980. Current-voltage analysis of apical sodium transport in toad urinary bladder: Effects of inhibitors of transport and metabolism. J. Membr. Biol. 57:59-71.
16. Palmer-Densmore, M., Deachapunya, C., Kannan, M., and O'Grady, S.M. 2002. UTP-dependent inhibition of Na+ absorption requires activation of PKC in endometrial epithelial cells. J. Gen. Physiol. 120:897-906.
17. Pietzonka, P., Walter, E., Duda-Johner, S., Langguth, P., and Merkle, H.P. 2002. Compromised integrity of excised porcine intestinal epithelium obtained from the abattoir affects the outcome of in vitro particle uptake studies. Eur. J. Pharm. Sci. 15:39-47.
18. Sheldon, R.J., Riviere, P.J.M., Malarchik, M.E., Mosberg, H.I., Burks, T.F., and Porreca, F. 1990. Opioid regulation of mucosal ion transport in the mouse isolated jejunum. J. Pharmacol. Exp. Ther. 253:144-151.
19. van de Kerkhof, E.G., Ungell, A.-L.B., Sjöberg, A.K., de Jager, M.H., Hilgendorf, C., de Graaf, I.A.M., and Groothuis, G.G. 2006. Innovative methods to study human drug metabolism in vitro: Precision-cut slices compared with Ussing chamber preparations. Drug Metab. Dispos. 34:1893-1902.
20. Vetter, A.E. and O'Grady, S.M. 1996. Mechanisms of electrolyte transport across the endometrium. I. Regulation by PGF2 alpha and cAMP. Am. J. Physiol. 270:C663-C672.
21. Wills,N.K., Lewis, S.A., and Eaton, D.C. 1979. Active and passive properties of rabbit descending colon: A microelectrode and nystatin study. J. Membr. Biol. 45:81-108.