The Syrian Hamster Embryo (SHE) low pH cell transformation assay

Current Protocols in Toxicology

Volumn , Issue SUPPL. 35, 2008, Pages

  Pant, Kamala ^a   Aardema, Marilyn J ^b  

^a BioReliance   (United States)

^b PROCTER AND GAMBLE COMPANY   (United States)

Author keywords

Clonal transformation; Colony transformation; Foci formation; In vitro carcinogenicity prediction; Morphological transformation; SHE

Indexed keywords

CARCINOGENESIS; CARCINOGENICITY; CELL ASSAY; CELL CULTURE; CELL ISOLATION; CELL PH; CELL STRUCTURE; CELL TRANSFORMATION; CHEMICAL PROCEDURES; CYTOTOXICITY; EMBRYO; EMBRYO CELL; NONHUMAN; OSMOLARITY; PRIORITY JOURNAL; REVIEW; SYRIAN HAMSTER;

ANIMALS; CARCINOGENICITY TESTS; CARCINOGENS; CELL TRANSFORMATION, NEOPLASTIC; CRICETINAE; EMBRYO CULTURE TECHNIQUES; EMBRYO, MAMMALIAN; HYDROGEN-ION CONCENTRATION; MESOCRICETUS;

MESOCRICETUS AURATUS;

EID: 57349118491     PISSN: 19349254     EISSN: 19349262     Source Type: Journal    
DOI: 10.1002/0471140856.tx2003s35     Document Type: Review

References (10)

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2. Custer, L., Gibson, D.P., Aardema, M.J., and LeBoeuf, R.A. 2000. A refined protocol for conducting the low pH 6.7 Syrian hamster embryo (SHE) cell transformation assay. Mut. Res. 455:129-139.
3. Isfort, R.J., Cody, D.B., Doerson, C., Kerckaert, G.A., and LeBoeuf, R.A. 1994. Alterations in cellular differentiation, mitogenesis, cytoskeleton and growth characteristics during Syrian hamster embryo cell multistep in vitro transformation. Int. J. Cancer 59:114-125.
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5. LeBoeuf, R.A. and Kerckaert, G.A. 1986. The induction of transformed-like morphology and enhanced growth in Syrian hamster embryo cells grown at acidic pH. Carcinogenesis 1986: 1431-1440.
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7. LeBoeuf, R.A., Kerckaert, G.A., Poiley, J.A., and Raineri, R. 1989. An interlaboratory comparison of enhanced morphological transformation of Syrian hamster embryo cells cultured under conditions of reduced bicarbonate concentration and pH. Mutat. Res. 222:205-218.
8. LeBoeuf, R.A., Kerckaert, G.A., Aardema, M.J., and Gibson, D.P. 1990. Multistage neoplastic transformation of Syrian hamster embryo cells cultured at pH6.70. Cancer Res. 50:3722-3729.
9. OECD. 2006. Detailed review paper on cell transformation assay for detection of chemical carcinogens. OECD Environment, Health, and Safety Publications Series on Testing and Assessment No. 31, Environment Directorate. OECD Publishing.
10. Pienta, R.J., Poiley, J.A., and Lebherb, W.G. III. 1977. Morphological transformation of early passage golden Syrian hamster embryo cells derived from cryopreserved primary cultures as a reliable in vitro bioassay for identifying diverse carcinogens. Int. J. Cancer 19:642-655.