Mechanisms and regulation of vitamin C uptake: Studies of the hSVCT systems in human liver epithelial cells

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 295, Issue 6, 2008, Pages

  Reidling, Jack C ^a   Subramanian, Veedamali S ^b   Dahhan, Tamara ^b   Sadat, Mohammed ^b   Said, Hamid M ^b  

^a VA LONG BEACH HEALTHCARE SYSTEM   (United States)

^b NONE

Author keywords

Ascorbic acid transport; Regulation by protein kinase C; Short interfering RNA

Indexed keywords

AMILORIDE; ASCORBIC ACID; COTRANSPORTER; DIHYDRO 4,4' DIISOTHIOCYANO 2,2' STILBENEDISULFONIC ACID; FUROSEMIDE; GLUCOCORTICOID; HYDROGEN; PROBENECID; PROTEIN HSVCT1; PROTEIN HSVCT2; PROTEIN KINASE C; SODIUM; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DOWN REGULATION; EPITHELIUM CELL; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LIVER CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION;

ASCORBIC ACID; BUDESONIDE; CELL LINE, TUMOR; DEHYDROASCORBIC ACID; DEXAMETHASONE; EPITHELIAL CELLS; GENE EXPRESSION REGULATION; HEPATOCYTES; HUMANS; LIVER; MIFEPRISTONE; ORGANIC ANION TRANSPORTERS, SODIUM-DEPENDENT; PROMOTER REGIONS, GENETIC; PROTEIN KINASE C; RNA, SMALL INTERFERING; SYMPORTERS; TETRADECANOYLPHORBOL ACETATE; TRANSFECTION;

EID: 57349113916     PISSN: 01931857     EISSN: 15221547     Source Type: Journal    
DOI: 10.1152/ajpgi.90399.2008     Document Type: Article

References (29)

1. Ashokkumar B, Vaziri ND, Said HM. Thiamin uptake by the human-derived renal epithelial (HEK-293) cells: cellular and molecular mechanisms. Am J Physiol Renal Physiol 291: F796-F805, 2006.
2. Balamurugan K, Vaziri ND, Said HM. Biotin uptake by human proximal tubular epithelial cells: cellular and molecular aspects. Am J Physiol Renal Physiol 288: F823-F831, 2005.
3. Banhegyi G, Marcolongo P, Puskas F, Fulceri R, Mandl J. Dehydroascorbate and ascorbate transport in rat liver microsomal vesicles. J Biol Chem 273: 2758-2762, 1998.
4. Biondi C, Pavan B, Dalpiaz A, Medici S, Lunghi L, Vesce F. Expression and characterization of vitamin C transporter in the human trophoblast cell line HTR-8/SVneo: effect of steroids, flavonoids and NSAIDs. Mol Hum Reprod 13: 77-83, 2007.
5. Carr AC, Frei B. Toward a new recommended dietary allowance for Vitamin C based on antioxidant and health effects in humans. Am J Clin Nutr 69: 1086-1107, 1999.
6. Daruwala R, Song J, Koh WS, Rumsey SC, Levine M. Cloning and functional characterization of the human sodium-dependent vitamin C transporters hSVCT1 and hSVCT2. FEBS Lett 460: 480-484, 1999.
7. Feschenko MS, Stevenson E, Sweadner KJ. Interaction of protein kinase C and cAMP-dependent pathways in the phosphorylation of the Na,K-ATPase. J Biol Chem 275: 34693-34700, 2000.
8. Fischer H, Schwarzer C, Illek B. Vitamin C controls the cystic fibrosis transmembrane conductance regulator chloride channel. Proc Natl Acad Sci USA 101: 3691-3696, 2004.
9. Fujita I, Hirano J, Itoh N, Nakanishi T, Tanaka K. Dexamethasone induces sodium-dependant vitamin C transporter in a mouse osteoblastic cell line MC3T3-E1. Br J Nutr 86: 145-149, 2001.
10. Harrison SA, Torgerson S, Hayashi P, Ward J, Schenker S. Vitamin E and vitamin C treatment improves fibrosis in patients with nonalcoholic steatohepatitis. Am J Gastroenterol 98: 2485-2490, 2003.
11. Kuo SM, MacLean ME, McCormick K, Wilson JX. Gender and sodium-ascorbate transporter isoforms determine ascorbate concentrations in mice. J Nutr 134: 2216-2221, 2004.
12. Li Y, Schellhorn HE. New developments and novel therapeutic perspectives for vitamin C. J Nutr 137: 2171-2184, 2007.
13. Liang WJ, Johnson D, Ma LS, Jarvis SM, Wei-Jun L. Regulation of the human vitamin C transporters expressed in COS-1 cells by protein kinase C. Am J Physiol Cell Physiol 283: C1696-C1704, 2002.
14. Maulen NP, Henriquez EA, Kempe S, Carcamo JG, Schmid-Kotsas A, Bachem M, Grunert A, Bustamante ME, Nualart F, Vera JC. Up-regulation and polarized expression of the sodium-ascorbic acid transporter SVCT1 in post-confluent differentiated CaCo-2 Cells. J Biol Chem 278: 9035-9041, 2003.
15. Michels AJ, Joisher N, Hagen TM. Age-related decline of sodium-dependent ascorbic acid transport in isolated rat hepatocytes. Arch Biochem Biophys 410: 112-120, 2003.
16. Packer L, Fuchs J. Vitamin C in health and disease. New York: Marcel Dekker, 1997.
17. Padayatty SJ, Doppman JL, Chang R, Wang Y, Gill J, Papanicolaou DA, Levine M. Human adrenal glands secrete vitamin C in response to adrenocorticotrophic hormone. Am J Clin Nutr 86: 145-149, 2007.
18. Rajan PD, Huang W, Dutta B, Devoe LD, Leibach FH, Ganapathy V, Prasad PD. Human placental sodium dependent vitamin C transporter (SVCT2): molecular cloning and transport function. Biochem Biophys Res Commun 262: 762-768, 1999.
19. Reidling JC, Nabokina SM, Balamurugan K, Said HM. Developmental maturation of intestinal and renal thiamin uptake: studies in wild-type and transgenic mice carrying human THTR-1 and -2 promoters. J Cell Physiol 206: 371-377, 2006.
20. +-dependent vitamin C transporter 2, SLC23A2, in human vascular smooth muscle cells. Biochim Biophys Acta 1732: 76-78, 2005.
21. Said HM, Balamurugan K, Subramanian VS, Marchant JS. Expression and functional contribution of hTHTR-2 in thiamin absorption in human intestine. Am J Physiol Gastrointest Liver Physiol 286: G491-G498, 2004.
22. Savini I, Rossi A, Pierro C, Avigliano L, Catani MV. SVCT1 and SVCT2: key proteins for vitamin C uptake. Amino Acids 34: 347-355, 2008.
23. Simon JA, Hudes ES. Serum ascorbic acid and gallbladder disease prevalence among US adults. Arch Intern Med 160: 931-936, 2000.
24. Subramanian VS, Marchant JS, Boulware MJ, Said HM. A C-terminal region dictates the apical plasma membrane targeting of the human sodium-dependent vitamin C transporter-1 in polarized epithelia. J Biol Chem 279: 27719-27728, 2004.
25. Takanaga H, Mackenzie B, Hediger MA. Sodium-dependent ascorbic acid transporter family SLC23. Pflügers Arch 447: 677-682, 2003.
26. Upston JM, Karjalainen A, Bygrave FL, Stocker R. Efflux of hepatic ascorbate: a potential contributor to the maintenance of plasma vitamin C. Biochem J 342: 49-56, 1999.
27. +-dependent vitamin C transporter 1 (hSVCT1): primary structure, functional characteristics and evidence for a nonfunctional splice variant. Biochim Biophys Acta 1461: 1-9, 1999.
28. Wang Y, Mackenzie B, Tsukaguchi H, Weremowicz S, Morton CC, Hediger MA. Human vitamin C (L-ascorbic acid) transporter SVCT1. Biochem Biophys Res Commun 267: 488-494, 2000.
29. Wilkinson GN. Statistical estimations in enzyme kinetics. Biochem J 80: 324-332, 1961.