Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform

Journal of Clinical Microbiology

Volumn 46, Issue 12, 2008, Pages 4049-4051

  Leblanc, Jason J ^a   Pettipas, Janice ^a   Davidson, Ross J ^a,b   Tipples, Graham A ^c   Hiebert, Joanne ^c   Hatchette, Todd F ^a,b  

^a QUEEN ELIZABETH II HEALTH SCIENCES CENTRE   (Canada)

^b DALHOUSIE UNIVERSITY   (Canada)

^c PUBLIC HEALTH AGENCY OF CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

RIBONUCLEASE P; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; COST BENEFIT ANALYSIS; DATA ANALYSIS; FUSION GENE; HUMAN; MUMPS VIRUS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; VIRUS DETECTION;

HUMANS; MUMPS; MUMPS VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBONUCLEASE P; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL FUSION PROTEINS;

MUMPS VIRUS;

EID: 57349108677     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01446-08     Document Type: Article

References (12)

1. Bloom, S., and M. Wharton. 2005. Mumps outbreak among young adults in UK. BMJ 331:E363-E364.
2. Boddicker, J. D., P. A. Rota, T. Kreman, A. Wangeman, L. Lowe, K. B. Hummel, R. Thompson, W. J. Bellini, M. Pentella, and L. E. DesJardin. 2007. Real-time reverse transcription-PCR assay for detection of mumps virus RNA in clinical samples. J. Clin. Microbiol. 45:2902-2908.
3. Cohen, C., J. M. White, E. J. Savage, J. R. Glynn, Y. Chol, N. Andrews, D. Brown, and M. E. Ramsay. 2007. Vaccine effectiveness estimates 2004-2005 mumps outbreak, England. Emerg. Infect. Dis. 13:12-17.
4. Jin, L., S. Beard, and D. W. Brown. 1999. Genetic heterogeneity of mumps virus in the United Kingdom: identification of two new genotypes. J. Infect. Dis. 180:829-833.
5. Jin, L., Y. Fengt, R. Parry, A. Cui, and Y. Lu. 2007. Real-time PCR and its application to mumps rapid diagnosis. J. Med. Virol. 79:1761-1767.
6. Krause, C. H., K. Eastick, and M. M. Ogilvie. 2006. Real-time PCR for mumps detection on clinical specimens - comparison with results of conventional methods of virus detection and nested PCR. J. Clin. Virol. 37:184-189.
7. Krause, C. H., P. J. Molyneaux, D. O. Ho-Yen, P. McIntyre, W. F. Carman, and K. E. Templeton. 2007. Comparison of mumps-IgM ELISAs in acute infection. J. Clin. Virol. 38:153-156.
8. Kubar, A., M. Yapar, B. Besirbellioglu, I. Y. Avci, and C. Guney. 2004. Rapid and quantitative detection of mumps virus RNA by one-step real-time RT-PCR. Diagn. Microbiol. Infect. Dis. 49:83-88.
9. Peltola, H., P. S. Kulkarni, S. V. Kapre, M. Paunio, S. S. Jadhav, and R. M. Dhere. 2007. Mumps outbreaks in Canada and the United States: time for new thinking on mumps vaccines. Clin. Infect. Dis. 45:459-466.
10. Sanz, J. C., M. D. M. Mosquera, J. E. Echevarria, M. Fernandez, N. Herranz, G. Palacios, and F. DeOry. 2006. Sensitivity and specificity of immunoglobulin G titer for the diagnosis of mumps virus in infected patients depending on vaccination status. APMIS 114:788.
11. Uchida, K., M. Shinohara, S. Shimada, Y. Segawa, R. Doi, A. Gotoh, and R. Hondo. 2005. Rapid and sensitive detection of mumps virus RNA directly from clinical samples by real-time PCR. J. Med. Virol. 75:470-474.
12. Watson-Creed, G., A. Saunders, J. Scott, L. Lowe, J. Pettipas, and T. F. Hatchette. 2006. Two successive outbreaks of mumps in Nova Scotia among vaccinated adolescents and young adults. Can. Med. Assoc. J. 175:483-488.